Acireductone synthase

Acireductone synthase (EC 3.1.3.77, E1, E-1 enolase-phosphatase) is an enzyme with systematic name 5-(methylthio)-2,3-dioxopentyl-phosphate phosphohydrolase (isomerizing).^[1]^[2]^[3] This enzyme catalyses the following chemical reaction

5-(methylthio)-2,3-dioxopentyl phosphate + H₂O

\rightleftharpoons

1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + phosphate (overall reaction)

(1a) 5-(methylthio)-2,3-dioxopentyl phosphate

\rightleftharpoons

2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate (probably spontaneous)

(1b) 2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate + H₂O

\rightleftharpoons

1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + phosphate

References

^ Myers, R.W.; Wray, J.W.; Fish, S.; Abeles, R.H. (1993). "Purification and characterization of an enzyme involved in oxidative carbon-carbon bond cleavage reactions in the methionine salvage pathway of Klebsiella pneumoniae". J. Biol. Chem. 268 (33): 24785–24791. PMID 8227039.
^ Wray, J.W.; Abeles, R.H. (1995). "The methionine salvage pathway in Klebsiella pneumoniae and rat liver. Identification and characterization of two novel dioxygenases". J. Biol. Chem. 270: 3147–3153. doi:10.1074/jbc.270.7.3147. PMID 7852397.{{cite journal}}: CS1 maint: unflagged free DOI (link)
^ Wang, H.; Pang, H.; Bartlam, M.; Rao, Z. (2005). "Crystal structure of human E1 enzyme and its complex with a substrate analog reveals the mechanism of its phosphatase/enolase activity". J. Mol. Biol. 348 (4): 917–926. doi:10.1016/j.jmb.2005.01.072. PMID 15843022.

Acireductone+synthase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)

v t e Enzymes
Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Diffusion-limited enzyme Cofactor Enzyme catalysis
Regulation	Allosteric regulation Cooperativity Enzyme inhibitor Enzyme activator
Classification	EC number Enzyme superfamily Enzyme family List of enzymes
Kinetics	Enzyme kinetics Eadie–Hofstee diagram Hanes–Woolf plot Lineweaver–Burk plot Michaelis–Menten kinetics
Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list) EC7 Translocases (list)