3-Ketosteroid reductase
| 3-keto-steroid reductase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 1.1.1.270 | ||||||||
| CAS no. | 42616-29-5 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
In enzymology, a 3-keto-steroid reductase (EC 1.1.1.270) is an enzyme that catalyzes the chemical reaction
- 4alpha-methyl-5alpha-cholest-7-en-3beta-ol + NADP+ 4alpha-methyl-5alpha-cholest-7-en-3-one + NADPH + H+
Thus, the two substrates of this enzyme are 4alpha-methyl-5alpha-cholest-7-en-3beta-ol and NADP+, whereas its 3 products are 4alpha-methyl-5alpha-cholest-7-en-3-one, NADPH, and H+.
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is 3beta-hydroxy-steroid:NADP+ 3-oxidoreductase. This enzyme is also called 3-KSR. This enzyme participates in biosynthesis of steroids.
References
- Billheimer JT, Alcorn M, Gaylor JL (1981). "Solubilization and partial purification of a microsomal 3-ketosteroid reductase of cholesterol biosynthesis". Arch. Biochem. Biophys. 211 (1): 430–8. doi:10.1016/0003-9861(81)90474-4. PMID 6946726.
- Swindell AC, Gaylor JL (1968). "Investigation of the component reactions of oxidative sterol demethylation. Formation and metabolism of 3-ketosteroid intermediates". J. Biol. Chem. 243 (21): 5546–55. PMID 4387005.