C64 or C-64 may refer to :
- Commodore 64, one of the most successful home computers of the 1980s
- C-64 (Michigan county highway), a road in the United States of America
- C-64 Norseman, an aircraft
- C64 (field gun), a late 19th-century field gun by Krupp
- Ruy Lopez, chess openings ECO code
- Renal cell carcinoma, ICD-10 code
- Commodore 64 (band), a Hip-hop group
- Caldwell 64 (NGC 2362), an open cluster in the constellation Canis Major
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