Lithium acetate

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Lithium acetate
Lithium acetate.png
Lithium acetate.JPG
Identifiers
546-89-4 YesY
ChEBI CHEBI:CHEBI:63045 N
ChemSpider 10562 N
EC Number 208-914-3
Jmol 3D model Interactive image
KEGG D08134 N
MeSH C488804
PubChem 11028
RTECS number AI545000
Properties
C2H3LiO2
Molar mass 65.98 g·mol−1
Appearance crystal
Density 1.26 g/cm3
Melting point 286 °C (547 °F; 559 K)
45.0 g/100 mL[1]
Hazards
Safety data sheet External MSDS
Lethal dose or concentration (LD, LC):
500 mg/kg (oral, mouse)
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
N verify (what is YesYN ?)
Infobox references

Lithium acetate (CH3COOLi) is a salt of lithium and acetic acid.

Uses[edit]

Lithium acetate is used in the laboratory as buffer for gel electrophoresis of DNA and RNA. It has a lower electrical conductivity and can be run at higher speeds than can gels made from TAE buffer (5-30V/cm as compared to 5-10V/cm). At a given voltage, the heat generation and thus the gel temperature is much lower than with TAE buffers, therefore the voltage can be increased to speed up electrophoresis so that a gel run takes only a fraction of the usual time. Downstream applications, such as isolation of DNA from a gel slice or Southern blot analysis, work as expected when using lithium acetate gels.

Lithium boric acid or sodium boric acid are usually preferable to lithium acetate or TAE when analyzing smaller fragments of DNA (less than 500 bp) due to the higher resolution of borate-based buffers in this size range as compared to acetate buffers.

Lithium acetate is also used to permeabilize the cell wall of yeast for use in DNA transformation. It is believed that the beneficial effect of LiOAc is caused by its chaotropic effect; denaturing DNA, RNA and proteins.[2]

References[edit]

  1. ^ Lide, David R. (1998). Handbook of Chemistry and Physics (87 ed.). Boca Raton, FL: CRC Press. p. 465. ISBN 0-8493-0594-2. 
  2. ^ Norcum, MT (Aug 15, 1991). "Structural analysis of the high molecular mass aminoacyl-tRNA synthetase complex. Effects of neutral salts and detergents.". The Journal of Biological Chemistry 266 (23): 15398–405. PMID 1651330.