This enzyme acts on the 3alpha-hydroxy group of androgens of the 5alpha-androstane series; and also, more slowly, on the 17alpha-hydroxy group of both androgenic and estrogenic substrates (cf. EC 22.214.171.124 3(or 17)beta-hydroxysteroid dehydrogenase).
^Lau, P.C.K.; Layne, D.S.; Williamson, D.G. (1982). "A 3(17)α-hydroxysteroid dehydrogenase of female rabbit kidney cytosol. Purification and characterization of multiple forms of the enzyme". J. Biol. Chem. 257: 9444–9449. PMID6955302.
^Lau, P.C.K.; Layne, D.S.; Williamson, D.G. (1982). "Comparison of the multiple forms of the soluble 3(17)α-hydroxysteroid dehydrogenases of female rabbit kidney and liver". J. Biol. Chem. 257: 9450–9456. PMID6955303.