3alpha(or 20beta)-hydroxysteroid dehydrogenase

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3-alpha(or 20-beta)-hydroxysteroid dehydrogenase
3-alpha,20-beta-hydroxysteroid dehydrogenase tetramer, Streptomyces exfoliatus
EC no.
CAS no.9028-42-6
IntEnzIntEnz view
ExPASyNiceZyme view
MetaCycmetabolic pathway
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO

In enzymology, a 3alpha(or 20beta)-hydroxysteroid dehydrogenase (EC is an enzyme that catalyzes the chemical reaction

androstan-3alpha,17beta-diol + NAD+ 17beta-hydroxyandrostan-3-one + NADH + H+

Thus, the two substrates of this enzyme are androstan-3alpha,17beta-diol and NAD+, whereas its 3 products are 17beta-hydroxyandrostan-3-one, NADH, and H+.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is 3alpha(or 20beta)-hydroxysteroid:NAD+ oxidoreductase. Other names in common use include cortisone reductase, (R)-20-hydroxysteroid dehydrogenase, dehydrogenase, 20beta-hydroxy steroid, Delta4-3-ketosteroid hydrogenase, 20beta-hydroxysteroid dehydrogenase, 3alpha,20beta-hydroxysteroid:NAD+-oxidoreductase, NADH-20beta-hydroxysteroid dehydrogenase, and 20beta-HSD. This enzyme participates in bile acid biosynthesis and c21-steroid hormone metabolism.

Structural studies[edit]

As of late 2007, 6 structures have been solved for this class of enzymes, with PDB accession codes 1HDC, 1N5D, 1NFF, 1NFQ, 1NFR, and 2HSD.


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  • Sweet F, Samant BR (1980). "Bifunctional Enzyme Activity at the Same Active Site: Study of 3α and 20β Activity by Affinity Alkylation of 3α,20β-Hydroxysteroid Dehydrogenase with 17-(Bromoacetoxy)steroids". Biochemistry. 19 (5): 978–86. doi:10.1021/bi00546a023. PMID 6928375.