Jump to content

Isoflavone 2'-hydroxylase

From Wikipedia, the free encyclopedia

This is an old revision of this page, as edited by Htmlzycq (talk | contribs) at 07:57, 10 October 2020. The present address (URL) is a permanent link to this revision, which may differ significantly from the current revision.

Isoflavone 2'-hydroxylase
Identifiers
EC no.1.14.14.90
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Search
PMCarticles
PubMedarticles
NCBIproteins

In enzymology, an isoflavone 2'-hydroxylase (EC 1.14.14.90, Formerly EC 1.14.13.89) is an enzyme that catalyzes the chemical reaction

an isoflavone + NADPH + H+ + O2 a 2'-hydroxyisoflavone + NADP+ + H2O

The 4 substrates of this enzyme are isoflavone, NADPH, H+, and O2, whereas its 3 products are 2'-hydroxyisoflavone, NADP+, and H2O.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of one atom o oxygen into the other donor. The systematic name of this enzyme class is isoflavone,NADPH:oxygen oxidoreductase (2'-hydroxylating). Other names in common use include isoflavone 2'-monooxygenase, CYP81E1, and CYP Ge-3. This enzyme participates in isoflavonoid biosynthesis.

References

  • Akashi T, Aoki T, Ayabe S (1998). "CYP81E1, a cytochrome P450 cDNA of licorice (Glycyrrhiza echinata L.), encodes isoflavone 2'-hydroxylase". Biochem. Biophys. Res. Commun. 251 (1): 67–70. doi:10.1006/bbrc.1998.9414. PMID 9790908.