Hypothetical types of biochemistry
Hypothetical types of biochemistry are forms of biochemistry speculated to be scientifically viable but not proven to exist at this time. The kinds of living beings currently known on Earth all use carbon compounds for basic structural and metabolic functions, water as a solvent and DNA or RNA to define and control their form. If life exists on other planets or moons, it may be chemically similar; it is also possible that there are organisms with quite different chemistries—for instance involving other classes of carbon compounds, compounds of another element, or another solvent in place of water.
The possibility of life-forms being based on "alternative" biochemistries is the topic of an ongoing scientific discussion, informed by what is known about extraterrestrial environments and about the chemical behaviour of various elements and compounds. It is also a common subject in science fiction.
The element silicon has been much discussed as a hypothetical alternative to carbon. Silicon is in the same group as carbon in the periodic table, and like carbon is tetravalent, although the silicon analogs of organic compounds are generally less stable. Hypothetical alternatives to water include ammonia, which, like water, is a polar molecule, and cosmically abundant; and non-polar hydrocarbon solvents such as methane and ethane, which are known to exist in liquid form on the surface of Titan.
- 1 Shadow biosphere
- 2 Alternative-chirality biomolecules
- 3 Non-carbon-based biochemistries
- 4 Arsenic as an alternative to phosphorus
- 5 Non-water solvents
- 6 Other types of speculations
- 7 Nonplanetary life
- 8 In fiction
- 9 Scientists who have published on this topic
- 10 See also
- 11 References
- 12 Further reading
- 13 External links
Apart from the prospect of finding different forms of life on other planets or moons, Earth itself has been suggested as a place where a shadow biosphere of biochemically unfamiliar micro-organisms might have lived in the past, or may still exist today.
Perhaps the least unusual alternative biochemistry would be one with differing chirality of its biomolecules. In known Earth-based life, amino acids are almost universally of the L form and sugars are of the D form. Molecules of opposite chirality have identical chemical properties to their mirrored forms, so life that used D amino acids or L sugars may be possible; molecules of such a chirality, however, would be incompatible with organisms using the opposing chirality molecules. Amino acids whose chirality is opposite to the norm are found on Earth, and these substances are generally thought to result from decay of organisms of normal chirality. However, physicist Paul Davies speculates that some of them might be products of "anti-chiral" life.
It is questionable, however, whether such a biochemistry would be truly alien. Although it would certainly be an alternative stereochemistry, molecules that are overwhelmingly found in one enantiomer throughout the vast majority of organisms can nonetheless often be found in another enantiomer in different (often basal) organisms such as in comparisons between members of Archea and other domains, making it an open topic whether an alternative stereochemistry is truly novel.
On Earth, all known living things have a carbon-based structure and system. Scientists have speculated about the pros and cons of using atoms other than carbon to form the molecular structures necessary for life, but no one has proposed a theory employing such atoms to form all the necessary structures. However, as Carl Sagan argued, it is very difficult to be certain whether a statement that applies to all life on Earth will turn out to apply to all life throughout the universe. Sagan used the term "carbon chauvinism" for such an assumption. Carl Sagan regarded silicon and germanium as conceivable alternatives to carbon; but, on the other hand, he noted that carbon does seem more chemically versatile and is more abundant in the cosmos.
The silicon atom has been much discussed as the basis for an alternative biochemical system, because silicon has many chemical properties similar to those of carbon and is in the same group of the periodic table, the carbon group. Like carbon, silicon can create molecules that are sufficiently large to carry biological information.
However, silicon has several drawbacks as an alternative to carbon. Silicon, unlike carbon, lacks the ability to form chemical bonds with diverse types of atoms as is necessary for the chemical versatility required for metabolism. Elements creating organic functional groups with carbon include hydrogen, oxygen, nitrogen, phosphorus, sulfur, and metals such as iron, magnesium, and zinc. Silicon, on the other hand, interacts with very few other types of atoms. Moreover, where it does interact with other atoms, silicon creates molecules that have been described as "monotonous compared with the combinatorial universe of organic macromolecules". This is because silicon atoms are much bigger, having a larger mass and atomic radius, and so have difficulty forming double bonds (the double bonded carbon is part of the carbonyl group, a fundamental motif of bio-organic chemistry).
Silanes, which are chemical compounds of hydrogen and silicon that are analogous to the alkane hydrocarbons, are highly reactive with water, and long-chain silanes spontaneously decompose. Molecules incorporating polymers of alternating silicon and oxygen atoms instead of direct bonds between silicon, known collectively as silicones, are much more stable. It has been suggested that silicone-based chemicals would be more stable than equivalent hydrocarbons in a sulfuric-acid-rich environment, as is found in some extraterrestrial locations.
Of the varieties of molecules identified in the interstellar medium as of 1998[update], 84 are based on carbon while only 8 are based on silicon. Moreover, of those 8 compounds, four also include carbon within them. The cosmic abundance of carbon to silicon is roughly 10 to 1. This may suggest a greater variety of complex carbon compounds throughout the cosmos, providing less of a foundation upon which to build silicon-based biologies, at least under the conditions prevalent on the surface of planets. Also, even though Earth and other terrestrial planets are exceptionally silicon-rich and carbon-poor (the relative abundance of silicon to carbon in the Earth's crust is roughly 925:1), terrestrial life is carbon-based. The fact that carbon is used instead of silicon, may be evidence that silicon is poorly suited for biochemistry on Earth-like planets. For example: silicon is less versatile than carbon in forming compounds; the compounds formed by silicon are unstable and it blocks the flow of heat.
Even so, biogenic silica is used by some Earth life, such as the silicate skeletal structure of diatoms. According to the clay hypothesis of A. G. Cairns-Smith, silicate minerals in water played a crucial role in abiogenesis: they replicated their crystal structures, interacted with carbon compounds, and were the precursors of carbon-based life.
Silicon compounds may possibly be biologically useful under temperatures or pressures different from the surface of a terrestrial planet, either in conjunction with or in a role less directly analogous to carbon. Polysilanols, the silicon compounds corresponding to sugars, are soluble in liquid nitrogen, suggesting that they could play in role in very low temperature biochemistry.
In cinematic and literary science fiction, at a moment when man-made machines cross from nonliving to living, it is often posited, this new form would be the first example of non-carbon-based life. Since the advent of the microprocessor in the late 1960s, these machines are often classed as computers (or computer-guided robots) and filed under "silicon-based life", even though the silicon backing matrix of these processors is not nearly as fundamental to their operation as carbon is for "wet life".
Other exotic element-based biochemistries
- Boranes are dangerously explosive in Earth's atmosphere, but would be more stable in a reducing environment. However, boron's low cosmic abundance makes it less likely as a base for life than carbon.
- Various metals, together with oxygen, can form very complex and thermally stable structures rivaling those of organic compounds; the heteropoly acids are one such family. Some metal oxides are also similar to carbon in their ability to form both nanotube structures and diamond-like crystals (such as cubic zirconia). Titanium, aluminium, magnesium, and iron are all more abundant in the Earth's crust than carbon. Metal-oxide-based life could therefore be a possibility under certain conditions, including those (such as high temperatures) at which carbon-based life would be unlikely. The Cronin group at Glasgow University reported self-assembly of tungsten polyoxometalates into cell-like spheres. By modifying their metal oxide content, he can give the spheres a hole as part of its structure and become a porous membrane, selectively allowing chemicals in and out of the sphere according to size.
- Sulfur is also able to form long-chain molecules, but suffers from the same high-reactivity problems as phosphorus and silanes. The biological use of sulfur as an alternative to carbon is purely hypothetical, especially because sulfur usually forms only linear chains rather than branched ones. (The biological use of sulfur as an electron acceptor is widespread and can be traced back 3.5 billion years on Earth, thus predating the use of molecular oxygen. Sulfur-reducing bacteria can utilize elemental sulfur instead of oxygen, reducing sulfur to hydrogen sulfide.)
Arsenic as an alternative to phosphorus
Arsenic, which is chemically similar to phosphorus, while poisonous for most life forms on Earth, is incorporated into the biochemistry of some organisms. Some marine algae incorporate arsenic into complex organic molecules such as arsenosugars and arsenobetaines. Fungi and bacteria can produce volatile methylated arsenic compounds. Arsenate reduction and arsenite oxidation have been observed in microbes (Chrysiogenes arsenatis). Additionally, some prokaryotes can use arsenate as a terminal electron acceptor during anaerobic growth and some can utilize arsenite as an electron donor to generate energy.
It has been speculated that the earliest life forms on Earth may have used arsenic in place of phosphorus in the structure of their DNA. A common objection to this scenario is that arsenate esters are so much less stable to hydrolysis than corresponding phosphate esters that arsenic would not be suitable for this function.
The authors of a 2010 geomicrobiology study, supported in part by NASA, have postulated that a bacterium, named GFAJ-1, collected in the sediments of Mono Lake in eastern California, can employ such 'arsenic DNA' when cultured without phosphorus. They proposed that the bacterium may employ high levels of poly-β-hydroxybutyrate or other means to reduce the effective concentration of water and stabilize its arsenate esters. This claim was heavily criticized almost immediately after publication for the perceived lack of appropriate controls. Science writer Carl Zimmer contacted several scientists for an assessment: "I reached out to a dozen experts ... Almost unanimously, they think the NASA scientists have failed to make their case". Other authors were unable to reproduce their results and showed that the NASA scientists had issues with phosphate contamination (3 μM), which could sustain extremophile lifeforms.
In addition to carbon compounds, all currently known terrestrial life also requires water as a solvent. This has led to discussions about whether water is the only liquid capable of filling that role. The idea that an extraterrestrial life-form might be based on a solvent other than water has been taken seriously in recent scientific literature by the biochemist Steven Benner, and by the astrobiological committee chaired by John A. Baross. Solvents discussed by the Baross committee include ammonia, sulfuric acid, formamide, hydrocarbons, and (at temperatures much lower than Earth's) liquid nitrogen, or hydrogen in the form of a supercritical fluid.
Carl Sagan once described himself as both a carbon chauvinist and a water chauvinist; however on another occasion he said he was a carbon chauvinist but "not that much of a water chauvinist". He considered hydrocarbons, hydrofluoric acid, and ammonia as possible alternatives to water.
Some of the properties of water that are important for life processes include a large temperature range over which it is liquid, a high heat capacity (useful for temperature regulation), a large heat of vaporization, and the ability to dissolve a wide variety of compounds. Water is also amphoteric, meaning it can donate and accept an H+ ion, allowing it to act as an acid or a base. This property is crucial in many organic and biochemical reactions, where water serves as a solvent, a reactant, or a product. There are other chemicals with similar properties that have sometimes been proposed as alternatives. Additionally, water has the unusual property of being less dense as a solid (ice) than as a liquid. This is why bodies of water freeze over but do not freeze solid (from the bottom up). If ice were denser than liquid water (as is true for nearly all other compounds), then large bodies of liquid would slowly freeze solid, which would not be conducive to the formation of life. Water as a compound is cosmically abundant, although much of it is in the form of vapour or ice. Subsurface liquid water is considered likely or possible on several of the outer moons: Enceladus (where geysers have been observed), Europa, Titan and Ganymede. Earth is the only world currently known to have stable bodies of liquid water on its surface.
Not all properties of water are necessarily advantageous for life, however. For instance, water ice has a high albedo, meaning that it reflects a significant quantity of light and heat from the Sun. During ice ages, as reflective ice builds up over the surface of the water, the effects of global cooling are increased.
There are some properties that make certain compounds and elements much more favorable than others as solvents in a successful biosphere. The solvent must be able to exist in liquid equilibrium over a range of temperatures the planetary object would normally encounter. Because boiling points vary with the pressure, the question tends not to be does the prospective solvent remain liquid, but at what pressure. For example, hydrogen cyanide has a narrow liquid phase temperature range at 1 atmosphere, but in an atmosphere with the pressure of Venus, with 92 bars (9.2 MPa) of pressure, it can indeed exist in liquid form over a wide temperature range.
The ammonia molecule (NH3), like the water molecule, is abundant in the universe, being a compound of hydrogen (the simplest and most common element) with another very common element, nitrogen. The possible role of liquid ammonia as an alternative solvent for life is an idea that goes back at least to 1954, when J.B.S. Haldane raised the topic at a symposium about life's origin.
Numerous chemical reactions are possible in an ammonia solution, and liquid ammonia has chemical similarities with water. Ammonia can dissolve most organic molecules at least as well as water does and, in addition, it is capable of dissolving many elemental metals. Haldane made the point that various common water-related organic compounds have ammonia-related analogs; for instance the ammonia-related amine group (-NH2) is analogous to the water-related alcohol group (-OH).
Ammonia, like water, can either accept or donate an H+ ion. When ammonia accepts an H+, it forms the ammonium cation (NH4+), analogous to hydronium (H3O+). When it donates an H+ ion, it forms the amide anion (NH2−), analogous to the hydroxide anion (OH−). Compared to water, however, ammonia is more inclined to accept an H+ ion, and less inclined to donate one; it is a stronger nucleophile. Ammonia added to water functions as Arrhenius base: it increases the concentration of the anion hydroxide. Conversely, using a solvent system definition of acidity and basicity, water added to liquid ammonia functions as an acid, because it increases the concentration of the cation ammonium. The carbonyl group (C=O), which is much used in terrestrial biochemistry, would not be stable in ammonia solution, but the analogous imine group (C=NH) could be used instead.
However, ammonia has some problems as a basis for life. The hydrogen bonds between ammonia molecules are weaker than those in water, causing ammonia's heat of vaporization to be half that of water, its surface tension to be a third, and reducing its ability to concentrate non-polar molecules through a hydrophobic effect. Gerald Feinberg and Robert Shapiro have questioned whether ammonia could hold prebiotic molecules together well enough to allow the emergence of a self-reproducing system. Ammonia is also flammable in oxygen, and could not exist sustainably in an environment suitable for aerobic metabolism. Though ammonia will only achieve combustion in oxygen in the presence of a platinum catalyst.
A biosphere based on ammonia would likely exist at temperatures or air pressures that are extremely unusual in relation to life on Earth. Life on Earth usually exists within the melting point and boiling point of water at normal pressure, between 0 °C (273 K) and 100 °C (373 K); at normal pressure ammonia's melting and boiling points are between −78 °C (195 K) and −33 °C (240 K). Chemical reactions generally proceed more slowly at a lower temperature. Therefore, ammonia-based life, if it exists, might metabolize more slowly and evolve more slowly than life on Earth. On the other hand, lower temperatures could also enable living systems to use chemical species which at Earth temperatures would be too unstable to be useful.
Ammonia and ammonia–water mixtures remain liquid at temperatures far below the freezing point of pure water, so such biochemistries might be well suited to planets and moons orbiting outside the water-based habitability zone. Such conditions could exist, for example, under the surface of Saturn's largest moon Titan.
Methane and other hydrocarbons
Methane (CH4) is a simple hydrocarbon: that is, a compound of two of the most common elements in the cosmos, hydrogen and carbon. It has a cosmic abundance comparable with ammonia. Hydrocarbons could act as a solvent over a wide range of temperatures, but would lack polarity. Isaac Asimov, the biochemist and science fiction writer, suggested in 1981 that poly-lipids could form a substitute for proteins in a non-polar solvent such as methane. Lakes composed of a mixture of hydrocarbons, including methane and ethane, have been detected on the surface of Titan by the Cassini spacecraft.
There is debate about the effectiveness of methane and other hydrocarbons as a solvent for life compared to water or ammonia. Water is a stronger solvent than the hydrocarbons, enabling easier transport of substances in a cell. However, water is also more chemically reactive, and can break down large organic molecules through hydrolysis. A life-form whose solvent was a hydrocarbon would not face the threat of its biomolecules being destroyed in this way. Also, the water molecule's tendency to form strong hydrogen bonds can interfere with internal hydrogen bonding in complex organic molecules. Life with a hydrocarbon solvent could make more use of hydrogen bonds within its biomolecules. Moreover, the strength of hydrogen bonds within biomolecules would be appropriate to a low-temperature biochemistry.
Astrobiologist Chris McKay has argued, on thermodynamic grounds, that if life does exist on Titan's surface, using hydrocarbons as a solvent, it is likely also to use the more complex hydrocarbons as an energy source by reacting them with hydrogen, reducing ethane and acetylene to methane. Possible evidence for this form of life on Titan was identified in 2010 by Darrell Strobel of Johns Hopkins University; a greater abundance of molecular hydrogen in the upper atmospheric layers of Titan compared to the lower layers, arguing for a downward diffusion at a rate of roughly 1025 molecules per second and disappearance of hydrogen near Titan's surface. As Strobel noted, his findings were in line with the effects Chris McKay had predicted if methanogenic life-forms were present. The same year, another study showed low levels of acetylene on Titan's surface, which were interpreted by Chris McKay as consistent with the hypothesis of organisms reducing acetylene to methane. While restating the biological hypothesis, McKay cautioned that other explanations for the hydrogen and acetylene findings are to be considered more likely: the possibilities of yet unidentified physical or chemical processes (e.g. a non-living surface catalyst enabling acetylene to react with hydrogen), or flaws in the current models of material flow. He noted that even a non-biological catalyst effective at 95 K would in itself be a startling discovery.
A hypothetical cell membrane capable of functioning in liquid methane in Titan conditions was computer-modeled in February 2015. Composed of small molecules that contain carbon, hydrogen, and nitrogen, it would have the same stability and flexibility as cell membranes on Earth, which are composed of phospholipids, compounds of carbon, hydrogen, oxygen, and phosphorus. This hypothetical cell membrane was termed an "azotosome", a portmanteau from "azote", French for nitrogen, and "liposome".
Hydrogen fluoride (HF), like water, is a polar molecule, and due to its polarity it can dissolve many ionic compounds. Its melting point is −84 °C and its boiling point is 19.54 °C (at atmospheric pressure); the difference between the two is a little more than 100 K. HF also makes hydrogen bonds with its neighbor molecules, as do water and ammonia. It has been considered as a possible solvent for life by scientists such as Peter Sneath and Carl Sagan.
The biota in an HF ocean could use the fluorine as an electron acceptor to photosynthesize energy. HF is dangerous to the systems of molecules that Earth-life is made of, but certain other organic compounds, such as paraffin waxes, are stable with it. Like water and ammonia, liquid hydrogen fluoride supports an acid-base chemistry. Using a solvent system definition of acidity and basicity, nitric acid functions as a base when it is added to liquid HF.
However, hydrogen fluoride, unlike water, ammonia and methane, is cosmically rare.
Hydrogen sulfide is the closest chemical analog to water, but is less polar and a weaker inorganic solvent. Hydrogen sulfide is quite plentiful on Jupiter's moon Io, and may be in liquid form a short distance below the surface; and astrobiologist Dirk Schulze-Makuch has suggested it as a possible solvent for life there. On a planet with hydrogen-sulfide oceans the source of the hydrogen sulfide could come from volcanos, in which case it could be mixed in with a bit of hydrogen fluoride, which could help dissolve minerals. Hydrogen sulfide life might use a mixture of carbon monoxide and carbon dioxide as their carbon source. They might produce and live off of sulfur monoxide which is analogous to oxygen or O2. Hydrogen sulfide, like hydrogen cyanide and ammonia, suffers from the small temperature range where it is liquid, though that, like that of hydrogen cyanide and ammonia, it increases with increasing pressure.
Silicon dioxide and silicates
Silicon dioxide, also known as glass, silica, or quartz, is very abundant in the universe and has a high range of temperature at where it is liquid. However, its melting point is 1,600 to 1,725 °C (2,912 to 3,137 °F), so it would be impossible to make organic compounds in that temperature, because all of them would decompose. Moreover, if the pressure increases, the melting point goes down. Silicates are similar to silicon dioxide and some could have lower boiling points than silica. Gerald Feinberg and Robert Shapiro have suggested that molten silicate rock could serve as a liquid medium for organisms with a chemistry based on silicon, oxygen, and other elements such as aluminum.
Other solvents or cosolvents
Other solvents sometimes proposed:
- Supercritical fluids: Supercritical carbon dioxide and supercritical hydrogen.
- Simple hydrogen compounds: hydrogen chloride.
- More complex compounds: sulfuric acid, formamide, methanol.
- Very-low-temperature fluids: liquid nitrogen, and hydrogen.
Sulfuric acid in liquid form is strongly polar. It remains liquid at higher temperatures than water, its liquid range being 10 °C to 337 °C at a pressure of 1 atm, although above 300 °C it will slowly decompose. Sulfuric acid is known to be abundant in the clouds of Venus, in the form of aerosol droplets. In a biochemistry that used sulfuric acid as a solvent, the alkene group (C=C), with two carbon atoms joined by a double bond, could function analogously to the carbonyl group (C=O) in water-based biochemistry.
A proposal has been made that life on Mars may exist and be using a mixture of water and hydrogen peroxide as its solvent. A 61.2% (by weight) mix of water and hydrogen peroxide has a freezing point of −56.5 °C, and also tends to super-cool rather than crystallize. It is also hygroscopic, an advantage in a water-scarce environment.
Supercritical carbon dioxide has been proposed as a candidate for alternative biochemistry due to its ability to selectively dissolve organic compounds and assist the functioning of enzymes and because "super-Earth"- or "super-Venus"-type planets with dense high-pressure atmospheres may be common.
Other types of speculations
Physicists have noted that, although photosynthesis on Earth generally involves green plants, a variety of other-colored plants could also support photosynthesis, essential for most life on Earth, and that other colors might be preferred in places that receive a different mix of stellar radiation than Earth. These studies indicate that, although blue photosynthetic plants would be less likely, yellow or red plants are plausible.
Many Earth plants and animals undergo major biochemical changes during their life cycles as a response to changing environmental conditions, for example, by having a spore or hibernation state that can be sustained for years or even millennia between more active life stages. Thus, it would be biochemically possible to sustain life in environments that are only periodically consistent with life as we know it.
For example, frogs in cold climates can survive for extended periods of time with most of their body water in a frozen state, whereas desert frogs in Australia can become inactive and dehydrate in dry periods, losing up to 75% of their fluids, yet return to life by rapidly rehydrating in wet periods. Either type of frog would appear biochemically inactive (i.e. not living) during dormant periods to anyone lacking a sensitive means of detecting low levels of metabolism.
Dust and plasma-based
In 2007, Vadim N. Tsytovich and colleagues proposed that lifelike behaviors could be exhibited by dust particles suspended in a plasma, under conditions that might exist in space. Computer models showed that, when the dust became charged, the particles could self-organize into microscopic helical structures capable of replicating themselves, interacting with other neighboring structures, and evolving into more stable forms. Similar forms of life were described in Fred Hoyle's classic novel The Black Cloud.
In the realm of science fiction, there have occasionally been forms of life proposed that, while often highly speculative and unsupported by rigorous theoretical examination, are nevertheless interesting and in some cases even plausible.
- Alternate chirality: In Arthur C. Clarke's short story "Technical Error", there is an example of differing chirality. This is not a case of alien life, rather it is an accident.
- Ammonoids: In Forward's Rocheworld series, an Earth-like biochemistry is proposed that uses a mixture of water and ammonia as its solvent.
- Cometoids: Gregory Benford and David Brin's Heart of the Comet features a comet with a conventional carbon-and-water-based ecosystem that becomes active near the perihelion when the Sun warms it.
- Cryomorphs: The Outsiders in Larry Niven's Known Space universe are cryogenic creatures based on liquid helium. They derive thermoelectric energy from a temperature gradient by basking half their body in sunlight, keeping the other half in shadow and exposed to interstellar vacuum.
- Dark-matter lifeforms: Stephen Baxter has, perhaps, imagined some of the most unusual exotic life-forms in his Xeelee series of novels and stories, including supersymmetric photino-based life that congregate in the gravity wells of stars, entities composed of quantum wave functions, and the Qax, who thrive in any form of convection cells, from swamp gas to the atmospheres of gas giants.
- Electromorphs: Brin's own novel Sundiver is an example of science fiction proposing a form of life existing within the plasma atmosphere of a star using complex self-sustaining magnetic fields.
- Gregory Benford had a form of plasma-based life exist in the accretion disk of a primordial black hole in his novel Eater.
- The suggestion that life could even occur within the plasma of a star has been picked up by other science fiction writers, as in David Brin's Uplift Saga or Frederik Pohl's novel The World at the End of Time. The idea is that places where reactions occur–even an incredible environment as a star–presents a possible medium for some chain of events that could produce a system able to replicate.
- Electromotes: Fred Hoyle's classic novel The Black Cloud features a life form consisting of a vast cloud of interstellar dust, the individual particles of which interact via electromagnetic signalling analogous to how the individual cells of multicellular terrestrial life interact.
- Outside science-fiction, life in interstellar dust has been proposed as part of the panspermia hypothesis. The low temperatures and densities of interstellar clouds would seem to imply that life processes would operate much more slowly there than on Earth. Inorganic dust-based life has been speculated upon based on recent computer simulations.
- Exomorphs: In James White's Sector General, a series of novels and short stories about a multi-environment hospital for the strangest life-forms imaginable, some of them breathing methane, chlorine, water, and sometimes also oxygen.
- Some of the species directly metabolise hard radiation and their environment does not differ much from the atmosphere of a star, whereas others live in near-absolute-zero temperatures.
- All life forms are classified according to their metabolism, internal and external features, and more extreme abilities (telepathy, empathy, hive mind, etc.) with four letter codes.
- Fluorocarbanoids: Robert L. Forward's Camelot 30K describes an ecosystem on the surface of Kuiper belt objects that is based on a fluorocarbon chemistry with OF2 as the principal solvent instead of H2O.
- The organisms in this ecology keep warm by secreting a pellet of uranium-235 inside themselves and then moderating its nuclear fission using a boron-rich carapace around it.
- Kuiper belt objects are known to be rich in organic compounds, such as tholins, so some form of life existing on their surfaces is not entirely implausible–though perhaps not going so far as to develop natural internal nuclear reactors, as have Forward's.
- Hyperversals: In the novel Diaspora, Egan describes lifeforms in the 6-D 'macrosphere' that use a collapsed-atom chemistry with energetic processes of the same order as nuclear reactions, due to the peculiarities of higher-dimensional physics.
- Infomorphs: In his novel Diaspora, Greg Egan posits entire virtual universes implemented on Turing Machines encoded by Wang Tiles in gargantuan polysaccharide 'carpets.'
- Lithomorphs: One of the major sentient species in Terry Pratchett's Discworld universe are the "Earth"-based (ranging from Detritus to Diamond) Trolls.
- Macromorphic planetoids: The sentient ocean that covers much of the surface of Solaris in Stanislaw Lem's eponymous novel also seems, from much of the fictional research quoted and discussed in the book, to be based on some element other than carbon.
- Mechanimorphs: In Stephen Baxter's book Manifold: Space, he also proposes natural robots, life forms made of iron, called the Gaijin, evolving from creatures in oceans of iron carbonyl.
- Methanoids: Alien warriors recruited by the god Klael in David Eddings' "Tamuli" trilogy are noted by their human opponents to breathe marsh-gas (methane). Within Eddings' universe, this limits their capacity for exertion in an oxygen atmosphere, and also determines the tactics used to fight them and eventually to destroy them in their encampments.
- Neutronimorphs: In Dragon's Egg and Starquake, Forward proposes life on the surface of a neutron star utilizing "nuclear chemistry" in the degenerate matter crust.
- Silicoids: John Clark, in the introduction to the 1952 shared-world anthology The Petrified Planet, outlined the biologies of the planet Uller, with a mixture of siloxane and silicone life, and of Niflheim, where metabolism is based on hydrofluoric acid and carbon tetrafluoride.
- This world-building defined the background for stories by Fletcher Pratt, H. Beam Piper, and Judith Merril.
- An example of silicon based life forms takes place in the Alan Dean Foster novel Sentenced to Prism in which the protagonist, Evan Orgell, is trapped on a planet whose entire ecosystem is mostly silicon-based.
- Similarly, Arthur C. Clarke's "Crusade" revolves around a planetwide life-form based on silicon and superfluid helium located in deep intergalactic space, processing its thoughts slowly by human standards, that sends probes to look for similar life in nearby galaxies. It concludes that it needs to make planets more habitable for similar life-forms, and sends out other probes to foment supernovae to do so.
- The webcomic Schlock Mercenary features a species, Carbosilicate Amorphs, evolved from self-repairing distributed data storage devices.
- Alternate biochemistry: In the Enterprise episode "The Communicator", an alien species is encountered whose blood chemistry, although not explicitly stated, is sufficiently different from terrestrial organisms that it is not red and iron is toxic to it.
- Electromorphs: Various Star Trek series also had episodes featuring photonic lifeforms.
- Lithomorphs: A well-known example of a non–carbon-based life-form in science fiction is the Horta in the original Star Trek episode "The Devil in the Dark". A highly intelligent silicon-based creature made almost entirely of pure rock, it tunnels through rock as easily as humans move through air. The entire species dies out every 50,000 years except for one who tends the eggs, which take the form of silicon nodules scattered throughout the caverns and tunnels of its home planet, Janus VI. The inadvertent destruction of many of these eggs by a human mining colony led the mother Horta to respond by killing the colonists and sabotaging their equipment; it was only through a Vulcan mind meld that the race's benevolence and intelligence were discovered and peaceful relations established.
- Lithomorphs: Star Trek would later offer other corporeal life-forms with an alternative biochemistry. The Tholians of "The Tholian Web" are depicted and described, in that episode and later in the Star Trek: Enterprise episode "In a Mirror, Darkly" as being primarily of mineral-based composition and thriving only in superheated conditions.
- Another episode from TOS's third season, "The Savage Curtain", depicted another rock creature called an Excalbian, which is believed in fanon to also have been silicon-based.
- In Star Trek: The Next Generation, the Crystalline Entity appeared in two episodes, "Datalore" and "Silicon Avatar". This was an enormous spacefaring crystal lattice that had taken thousands of lives in its quest for energy. It may have been unaware of this, however, but it was destroyed before communications could be established at a level sufficient to ascertain it.
- In another episode, "Home Soil", intelligent crystals that formed a "microbrain" were discovered during a terraforming mission, and they described the humans they encountered as "ugly bags of mostly water."
- Silicoids: "The Disease", an episode of Star Trek: Voyager featured some artificially engineered silicon-based parasites, and an Enterprise episode, "Observer Effect", also presented a lethal silicon-based virus.
- Ammonoids: In the The Empire Strikes Back, the bounty hunter Zuckuss is a member of the Gand race, an ammonia-based life-form. However, it is worth noting that the Gand are divided into two subspecies, only one of which breathes at all, the other drawing all their required sustenance from food intake and producing speech by essentially modulated flatulence.
- Hyperversals: Appearing only in the Star Wars Expanded Universe is the spice spider of Kessel, a creature made of glitterstim spice and silicon that spun crystalline webs harvested by miners as glitterstim spice, an illegal psychoactive narcotic. The spider used the webs to catch bogeys, tiny energy creatures that it consumed for energy.
- Silicoids: In the Star Wars movie The Empire Strikes Back, two life-forms were encountered by the characters that were non-carbon based entities.
- Although details of their physiology were not mentioned on screen, the space slug, (a giant worm-like creature that lived on asteroids in the vacuum of space), and the Mynock, (pesky bat-like vermin that would attach to spaceship hulls and chew through power conduits to feed off the raw energy), are said to be silicon-based organisms in Star Wars Expanded Universe sources.
Other film and television
- Ammonoids: Also from The X-Files, the first-season episode "Ice" deals with an ammonia-based vermiform parasite.
- Calcimorphs: In Doctor Who 's first series after its revival, episodes 4–5 and 11 featured the alien Slitheen family of the planet Raxicoricofallapatorius, which were said to be calcium-based life forms, causing them to be blown up in contact with vinegar.
- The Slitheen family also reappeared in several episodes in The Sarah Jane Adventures series, as well as cameo appearances in later Doctor Who episodes.
- Hyperversals: Indiana Jones and the Kingdom of the Crystal Skull (2008) introduces thirteen "extra dimensional beings" with crystal skeletons, who founded a city that became the basis of the El Dorado myth. Though their flesh has died and rotted away, their minds still live on within their skeletons, which communicate telepathically.
- Nitrogen-based lifeforms: A key plot point in the comedy Evolution involves nitrogen-based life forms, and using selenium-based shampoo to poison them (with the bonus of a product placement for Head & Shoulders).
- Silicoids: In the movie The Monolith Monsters (1957), a silicon meteor reproduces, draining silicates from everything it touches. It needs water to start its cycle and contains molecular structures typical of many kinds of rocks, mixed together. A geologist says that its structure is nearly impossible. The meteor is killed by salt water, which can stop the cycle.
- On the Island of Terror (1966), silicon based life-forms are created accidentally by scientists while researching a cure for cancer. The "Silicates" are not pretty, they feed on bones (human or otherwise) and they can reproduce by mitosis. Not much to say about the scientific side though, since the survivors ignore what the creatures are exactly made of. They eventually use a very '60s way to defeat them.
- In "Firewalker", a second-season episode of The X-Files, a silicon-based plant that infects humans parasitically through its spores are discovered living deep in a volcano.
- In the Stargate Atlantis fifth season episode "Remnants", a device is found whose purpose was to seed a planet with silicon-based life.
- The episode "The Stones of Blood", of the 16th season of Doctor Who, the Fourth Doctor encounters the Ogri, a silicon-based life form, and in the same sub-plot, the Megara, who are made entirely out of an unknown substance, possibly energy, and they uphold the word of the law, and execute all who break the law with a beam of energy.
- The Kaiju in Pacific Rim are silicon-based lifeforms whose blood is ammonia-based and highly toxic.
- The humanoid Cylons in the reimagined Battlestar Galactica, although very similar to "original" humans, have silica-based nervous systems due to their origins as robots. This subtle difference has varying effects, such as susceptibility to radiation that would not affect carbon-based lifeforms.
- Sulfur-based: In the Stargate SG-1 fourth season episode "Scorched Earth", a human society known as the Enkarans are threatened on their new homeworld by an alien ship that is terraforming the planet to be suitable for the sulfur-based Gadmeer species.
Computer and video games
- Alternate chirality: In Mass Effect the alien Turians and Quarians are both based on dextro-amino acids, as opposed to all the other sentient species of the galaxy based on levo-amino acids. There are also the Volus, an ammonia based species that must wear pressure suits to survive in environments suited to the other races.
- Lithomorphs: In the Command & Conquer real-time strategy games, both the gameplay and storyline revolve heavily around the introduction to Earth, via meteor, of the extraterrestrial mutagen Tiberium, which displays strikingly lifelike behaviours, such as self-replication, evolution, and homeostasis, without undergoing anything like common carbon-based metabolic cycles, and that appears to be colonising Earth, converting it into an environment unsuited to carbon-based biology.
- Earth creatures (such as animals, plants and even humans) exposed to Tiberium can either be killed because of the radiation or be transformed into Tiberium-based lifeforms, to whom Tiberium radiation is curative rather than toxic.
- It is later revealed that Tiberium was introduced to Earth by the Scrin, an extremely advanced race of Tiberium-based aliens bent on mining the planet after the Tiberium deposits have reached maturity.
- In the Metroid Prime series, Phazon is a highly radioactive, self-regenerating mineral with organic properties that is generated by the sentient planet Phaaze.
- In Metroid Prime Hunters, Spire is a rock-like, silicon-based alien. He is the last Diamont (presumably a play on the word diamond, which is composed of carbon).
- Methanoids: In the Halo franchise, the weak, low-ranking Grunts of the Covenant originate on a frozen exoplanet named Balaho, where methane is a primary constituent of the atmosphere, preventing the planet becoming even more frigid than it already is due to its distance from its parent star, and thus, Grunts have evolved to utilize the gas for respiration. Grunts are also shown to be able to use benzene as a recreational drug.
- Silicoids: In the Master of Orion series of space strategy games, there exists an extraterrestrial race called Silicoids, whose appearance (and presumably composition) is similar to crystalline mineral structures. The game posits that this grants them immunity to the effects of hostile environments and pollution and they require no sustenance, at the expense of impeding their reproductive rate and their ability to interact with other intelligent species.
- In the Star Control series, the Chenjesu are hyperintelligent, peaceful silicon-based life-forms that were the backbone of the Alliance of Free Stars. Their crystalline biology apparently gives them the ability to send and receive hyperwave transmissions.
- In the Star Control series, there are the Slylandro, who are gas beings residing in the upper atmosphere of a gas giant. As well, there are evidences of another silicon-based race, the Taalo who are described by the xenophobic Ur-Quan as the only race to have not awakened their territorial instincts. The Taalo were also immune to mind control.
- In the game of Xenosaga, artificial life forms known as Realians have been created using silicon-based chemistry. They resemble humans in every aspect, except they are considered to be lower than humans on the social ladder.
- In Spore, the Grox refer to the player and to other alien empires as "slow thinking carbon-based lifeforms" and "carbon wads", implying that the Grox (which are at least partly machine life) are not carbon-based. Also, the Grox can only exist on barren planets which cannot support other life, and when a planet is terraformed the Grox inhabiting it die immediately. The Grox seem to gather sustenance from the radiation from the galactic core, as the Grox colonies are larger the closer they are to the galactic core.
- In Muv Luv, the BETA which calls itself the "higher/superior existence" says they were created by a silicon-based being simply called "The Creator". As such, they don't consider any non-silicon-based creature to be alive, not even themselves. Its reasoning was that only silicon-based beings occur naturally and have the ability to reproduce and disperse. When the human main character, Takeru, argues that humans also have the ability to reproduce and disperse, the higher existence says carbon too easily mingles with other elements and therefore it would be impossible for a carbon-based existence to have evolved on its own. Thus, humans must be other biological machines created by a life form just as the BETA are.
Scientists who have published on this topic
Scientists who have considered possible alternatives to carbon-water biochemistry include:
- Isaac Asimov (1920–1992), biochemist and science fiction writer.
- William Bains, Cambridge biologist, a contributor to the journal Astrobiology.
- John Baross, oceanographer and astrobiologist, who chaired a committee of scientists under the United States National Research Council which in 2007 published a report on life's limiting conditions. The report addresses the concern that a space agency might conduct a well-resourced search for life on other worlds "and then fail to recognize it if it is encountered".
- Gerald Feinberg (1933–1992), physicist and Robert Shapiro (1935–2011), chemist, co-authors of the book Life Beyond Earth.
- V.Axel Firsoff (1910–1981), British astronomer.
- Robert A. Freitas Jr. (1952-Present), specialist in nano-technology and nano-medicine; author of the book Xenology.
- J. B. S. Haldane (1892–1964), a geneticist noted for his work on abiogenesis.
- George Pimentel (1922–1989), American chemist, University of California, Berkeley.
- Carl Sagan (1934–1996), astronomer, science popularizer, and SETI proponent.
- Peter Sneath (1923–2011), microbiologist, author of the book Planets and Life.
- David J. Des Marais et al. (2008). "The NASA Astrobiology Roadmap" (PDF). Astrobiology 8 (4): 718. doi:10.1089/2008.0819.
- Davila, Alfonso F.; McKay, Christopher P. (May 27, 2014). "Chance and Necessity in Biochemistry: Implications for the Search for Extraterrestrial Biomarkers in Earth-like Environments". Astrobiology 14 (6): 534–540. doi:10.1089/ast.2014.1150. Retrieved 2015-03-29.
- Davies, P. C. W. , Benner, S.A., Cleland, C.E., Lineweaver,C.H., McKay,C.P. and Wolfe-Simon,F. Signatures of a Shadow Biosphere (2009) Astrobiology. 9(2): 241–249. doi:10.1089/ast.2008.0251.
- Cleland, Carol E.; Copley, Shelley D. (16 January 2006). "The possibility of alternative microbial life on Earth". International Journal of Astrobiology 4 (3-4): 165. doi:10.1017/S147355040500279X. archived at  (2009-03-20) from original 
- P.C.W. Davies and Charles H. Lineweaver (2005). "Hypothesis Paper: Finding a Second Sample of Life on Earth" (PDF). Astrobiology 5 (2): 159. doi:10.1089/ast.2005.5.154.
- Sagan, Carl; Agel, Jerome (2000). Carl Sagan's Cosmic Connection: an Extraterrestrial Perspective (2nd ed.). Cambridge U.P. p. 41. ISBN 9780521783033.
- Sagan, Carl (2000). Carl Sagan's Cosmic Connection: an Extraterrestrial Perspective (2nd ed.). Cambridge U.P. p. 46.
- Sagan, Carl (2000). Carl Sagan's Cosmic Connection: an Extraterrestrial Perspective (2nd ed.). Cambridge U.P. p. 47.
- Pace, NR (2001). "The universal nature of biochemistry" (PDF). Proceedings of the National Academy of Sciences of the United States of America 98 (3): 805–8. Bibcode:2001PNAS...98..805P. doi:10.1073/pnas.98.3.805. PMC 33372. PMID 11158550.
- Gillette, Stephen. World-Building. Writer's Digest Books. ISBN 0-89879-707-1.
- Lazio, Joseph. "F.10 Why do we assume that other beings must be based on carbon? Why couldn't organisms be based on other substances?". [sci.astro] ET Life (Astronomy Frequently Asked Questions). Retrieved 2006-07-21.
- "Astrobiology". Biology Cabinet. September 26, 2006. Retrieved 2011-01-17.
- Cairns-Smith, A. Graham (1985). Seven Clues to the Origin of Life. Cambridge: Cambridge University Press. ISBN 0-521-27522-9.
- Dawkins, Richard (1996) . The Blind Watchmaker. New York: W. W. Norton & Company, Inc. pp. 148–161. ISBN 0-393-31570-3.
- William Bains. "Astrobiology—the nature of life". WilliamBains.co.uk. Retrieved 2015-03-20.
- William Bains (June 2004). "Many Chemistries Could Be Used to Build Living Systems". Astrobiology 4 (2): 137–167. doi:10.1089/153110704323175124. PMID 15253836.
- "Life-like cells are made of metal". New Scientist. September 14, 2011. Retrieved 2014-05-25.
- Early Archaean Microorganisms Preferred Elemental Sulfur, Not Sulfate Science AAAS, by Philippot, et al., (14 September 2007)
- "Biochemical Periodic Table – Arsenic". UMBBD. 2007-06-08. Retrieved 2010-05-29.
- Niggemyer, A; Spring S; Stackebrandt E; Rosenzweig RF (December 2001). "Isolation and characterization of a novel As(V)-reducing bacterium: implications for arsenic mobilization and the genus Desulfitobacterium". Appl Environ Microbiol 67 (12): 5568–80. doi:10.1128/AEM.67.12.5568-5580.2001. PMC 93345. PMID 11722908.
- Reilly, Michael (26 April 2008). "Early life could have relied on 'arsenic DNA'". New Scientist 198 (2653): 10. doi:10.1016/S0262-4079(08)61007-6.
- Westheimer, F. H. (1987-03-06). "Why nature chose phosphates" (PDF). Science 235 (4793): 1173–1178 (see pp. 1175–1176). Bibcode:1987Sci...235.1173W. doi:10.1126/science.2434996. Retrieved 2010-12-03.
- "NASA-Funded Research Discovers Life Built With Toxic Chemical". NASA.gov. 2 December 2010. Retrieved 2010-12-02.
- Wolfe-Simon, Felisa; Blum, Jodi Switzer; Kulp, Thomas R.; Gordon, Shelley E.; Hoeft, S. E.; Pett-Ridge, Jennifer; Stolz, John F.; Webb, Samuel M.; Weber, Peter K.; Davies, Paul C. W.; Anbar, Ariel D.; Oremland, Ronald S. (2 December 2010). "A Bacterium That Can Grow by Using Arsenic Instead of Phosphorus" (PDF). Science 332 (6034): 1163–6. Bibcode:2011Sci...332.1163W. doi:10.1126/science.1197258. PMID 21127214. Retrieved 2010-12-09.
- Redfield, Rosemary (4 December 2010). "Arsenic-associated bacteria (NASA's claims)". RR Research blog. Retrieved 4 December 2010.
- Bradley, Alex (5 December 2010). "Arsenate-based DNA: a big idea with big holes". We, Beasties blog. Retrieved 9 December 2010.
- Zimmer, Carl (7 December 2010). "Scientists see fatal flaws in the NASA study of arsenic-based life". Slate. Retrieved 7 December 2010.
- Williams, Sarah (7 November 2012). ""Arsenic Life" Claim Refuted". BioTechniques. Retrieved 23 January 2013.
- Benner, Steven A.; Ricardo, Alonso; Carrigan, Matthew A (2004). "Is there a common chemical model for life in the universe?". Current Opinion in Chemical Biology 8 (6): 676–680. doi:10.1016/j.cbpa.2004.10.003. PMID 15556414.Text as pdf from www.sciencedirect.com (accessed 13 July 2011)
- Committee on the Limits of Organic Life in Planetary Systems, Committee on the Origins and Evolution of Life, National Research Council; The Limits of Organic Life in Planetary Systems; The National Academies Press, 2007; pages 69–79
- Committee on the Limits of Organic Life in Planetary Systems, Committee on the Origins and Evolution of Life, National Research Council; The Limits of Organic Life in Planetary Systems; The National Academies Press, 2007; p 72
- Committee on the Limits of Organic Life in Planetary Systems, Committee on the Origins and Evolution of Life, National Research Council; The Limits of Organic Life in Planetary Systems; The National Academies Press, 2007; p 73
- Committee on the Limits of Organic Life in Planetary Systems, Committee on the Origins and Evolution of Life, National Research Council; The Limits of Organic Life in Planetary Systems; The National Academies Press, 2007; p 74
- Committee on the Limits of Organic Life in Planetary Systems, Committee on the Origins and Evolution of Life, National Research Council; The Limits of Organic Life in Planetary Systems; The National Academies Press, 2007; p 75
- Sagan, Carl (2002). Cosmos. Random House. pp. 126–127. ISBN 0-375-50832-5.
- Sagan, Carl; Head, Tom (2006). Conversations with Carl Sagan. University Press of Mississippi. p. 10. ISBN 1-57806-736-7.
- Sagan, Carl (2002). Cosmos. Random House. p. 128. ISBN 0-375-50832-5.
- Committee on the Limits of Organic Life in Planetary Systems, Committee on the Origins and Evolution of Life, National Research Council; The Limits of Organic Life in Planetary Systems; The National Academies Press, 2007; page 70
- Isaac Asimov (Winter 1981). "Not as We Know it – the Chemistry of Life". Cosmic Search (North American AstroPhysical Observatory) (9 (Vol 3 No 1)).
- J.B.S. Haldane (1954). "The Origins of Life". New Biology 16: 12–27. cited in Darling, David. "ammonia-based life". Retrieved 2012-10-01.
- Darling, David. "ammonia-based life". Retrieved 2012-10-01.
- Feinberg, Gerald; Robert Shapiro (1980). Life Beyond Earth. Morrow. ISBN 0688036422. ISBN 9780688036423. cited in Darling, David. "ammonia-based life". Retrieved 2012-10-01.
- Schulze-Makuch, Dirk; Irwin, Louis Neal (2008). Life in the Universe: Expectations and Constraints (2 ed.). Springer. p. 119. ISBN 9783540768166.
- Fortes, A. D. (1999). "Exobiological Implications of a Possible Ammonia-Water Ocean Inside Titan". Retrieved 7 June 2010.
- Committee on the Limits of Organic Life in Planetary Systems, Committee on the Origins and Evolution of Life, National Research Council; The Limits of Organic Life in Planetary Systems; The National Academies Press, 2007; page 74.
- McLendon, Christopher; Opalko, F. Jeffrey (March 2015). "Solubility of Polyethers in Hydrocarbons at Low Temperatures. A Model for Potential Genetic Backbones on Warm Titans". Astrobiology 15 (3): 200–206. Bibcode:2015AsBio..15..200M. doi:10.1089/ast.2014.1212. Retrieved 2015-03-25.
- "What is Consuming Hydrogen and Acetylene on Titan?". NASA/JPL. 2010. Retrieved 2010-06-06.
- McKay, C. P.; Smith, H. D. (2005). "Possibilities for methanogenic life in liquid methane on the surface of Titan". Icarus 178 (1): 274–276. Bibcode:2005Icar..178..274M. doi:10.1016/j.icarus.2005.05.018.
- Strobel, Darrell F. (2010). "Molecular hydrogen in Titan's atmosphere: Implications of the measured tropospheric and thermospheric mole fractions" (PDF). Icarus 208 (2): 878–886. Bibcode:2010Icar..208..878S. doi:10.1016/j.icarus.2010.03.003.
- Mckay, Chris (2010). "Have We Discovered Evidence For Life On Titan". New Mexico State University, College of Arts and Sciences, Department of Astronomy. Retrieved 2014-05-15.
- Life 'not as we know it' possible on Saturn's moon Titan
- Sneath, P.H.A. (1970). Planets and Life. Thames and Hudson. cited in Boyce, Chris (1981). Extraterrestrial Encounter. New English Library. pp. 125, 182.
- Jander, Gerhart; Spandau, Hans; Addison, C.C. (1971). Chemistry in Nonaqueous Ionizing solvents: Inorganic Chemistry in Liquid Hydrogen Cyanide and Liquid hydrogen Fluoride II. N.Y.: Pergamon Press. cited in Freitas, Robert A. (1979). "8.2.2". Xenology: An Introduction to the Scientific Study of Extraterrestrial Life, Intelligence, and Civilization. Sacramento, CA: Xenology Research Institute.
- Freitas, Robert A. (1979). "8.2.2". Xenology: An Introduction to the Scientific Study of Extraterrestrial Life, Intelligence, and Civilization. Sacramento, CA: Xenology Research Institute.
- Darling, David. "solvent". Retrieved 2012-10-12.
- Jander, J.; Lafrenz, C. (1970). Ionizing Solvents I. Weinheim/Bergstr.: John Wiley & Sons Ltd., Verlag Chemie. cited in Freitas, Robert A. (1979). "8.2.2". Xenology: An Introduction to the Scientific Study of Extraterrestrial Life, Intelligence, and Civilization. Sacramento, CA: Xenology Research Institute.
- Choi, Charles Q. "The Chance for Life on Io". Retrieved 2013-05-25.
- David W. Koerner and Simon LeVay (2000). Here Be Dragons : The Scientific Quest for Extraterrestrial Life:. Oxford U.P. p. 202. ISBN 9780198033370.
- Budisa, Nediljko; Schulze-Makuch, Dirk (8 August 2014). "Supercritical Carbon Dioxide and Its Potential as a Life-Sustaining Solvent in a Planetary Environment". Life 4 (3): 331–340. doi:10.3390/life4030331.
- Ward, Peter D.; Benner, Steven A. (2007). "Alien biochemistries". In Sullivan, Woodruff T.; Baross, John A. Planets and Life. Cambridge: Cambridge. p. 540. ISBN 978-0521531023.
- Houtkooper, Joop M.; Dirk Schulze-Makuch (2007-05-22). "A Possible Biogenic Origin for Hydrogen Peroxide on Mars". International Journal of Astrobiology 6 (2): 147. arXiv:physics/0610093. Bibcode:2007IJAsB...6..147H. doi:10.1017/S1473550407003746.
- Houtkooper, Joop M.; Dirk Schulze-Makuch (2007). "The H2O2-H2O Hypothesis: Extremophiles Adapted to Conditions on Mars?" (PDF). EPSC Abstracts (European Planetary Science Congress 2007) 2: 558. Bibcode:2007epsc.conf..558H. EPSC2007-A-00439.
- Ellison, Doug (2007-08-24). "Europlanet : Life's a bleach". Planetary.org.
- "NASA – NASA Predicts Non-Green Plants on Other Planets". Nasa.gov. 2008-02-23. Retrieved 2010-05-29.
- Kiang, Nancy Y.; Segura, Antígona; Tinetti, Giovanna; Jee, Govind; Blankenship, Robert E.; Cohen, Martin; Siefert, Janet; Crisp, David; Meadows, Victoria S. (2007-04-03). "Spectral signatures of photosynthesis. II. Coevolution with other stars and the atmosphere on extrasolar worlds". Astrobiology (Mary Ann Liebert, Inc.) 7 (1): 252–274. arXiv:astro-ph/0701391. Bibcode:2007AsBio...7..252K. doi:10.1089/ast.2006.0108. PMID 17407410. Retrieved 2010-12-03.
- "Christmas in Yellowstone". Pbs.org. Retrieved 2010-05-29.
- Main and Bentley, Ecology, "Water Relations of Australian Burrowing Frogs and Tree Frogs" (1964)
- "Physicists Discover Inorganic Dust With Lifelike Qualities". Science Daily. 2007-08-15.
- Tsytovich, V N; G E Morfill, V E Fortov, N G Gusein-Zade, B A Klumov and S V Vladimirov; Fortov, V E; Gusein-Zade, N G; Klumov, B A; Vladimirov, S V (14 August 2007). "From plasma crystals and helical structures towards inorganic living matter". New J. Phys. 9 (263): 263. Bibcode:2007NJPh....9..263T. doi:10.1088/1367-2630/9/8/263.
- SciFi.com StarTrek Syfy[dead link]
- Battlestar Galactica (TV miniseries)
- W. Bains (2004). "Many Chemistries Could Be Used to Build Living Systems". Astrobiology 4 (2): 137–167. Bibcode:2004AsBio...4..137B. doi:10.1089/153110704323175124. PMID 15253836.
- Committee on the Limits of Organic Life in Planetary Systems, Committee on the Origins and Evolution of Life, National Research Council; The Limits of Organic Life in Planetary Systems; The National Academies Press, 2007.
- Committee on the Limits of Organic Life in Planetary Systems, Committee on the Origins and Evolution of Life, National Research Council; The Limits of Organic Life in Planetary Systems; The National Academies Press, 2007; page 5
- Feinberg, Gerald; Robert Shapiro (1980). Life Beyond Earth. Morrow. ISBN 0688036422.
- A detailed review of this book is: John Gribbin (2 Oct 1980). "Life beyond Earth". New Scientist: xvii.
- V. Axel Firsoff (January 1962). "An Ammonia-Based Life". Discovery 23: 36–42. cited in Darling, David. "ammonia-based life". Retrieved 2012-10-01.
- Freitas, Robert A. (1979). Xenology: An Introduction to the Scientific Study of Extraterrestrial Life, Intelligence, and Civilization. Sacramento, CA: Xenology Research Institute.
- This work is acknowledged the partial basis of the article Darling, David. "ammonia-based life". Retrieved 2012-10-01.
- Shklovskii, I.S.; Carl Sagan (1977). Intelligent Life in the Universe. Picador. p. 229.
- W. Bains (2004). "Many Chemistries Could Be Used to Build Living Systems". Astrobiology 4 (2): 137–167. Bibcode:2004AsBio...4..137B. doi:10.1089/153110704323175124. PMID 15253836.