2,6-beta-fructan 6-levanbiohydrolase (EC3.2.1.64, beta-2,6-fructan-6-levanbiohydrolase, 2,6-beta-D-fructan 6-levanbiohydrolase, levanbiose-producing levanase, 2,6-beta-D-fructan 6-beta-D-fructofuranosylfructohydrolase) is an enzyme with systematic name(2->6)-beta-D-fructofuranan 6-(beta-D-fructosyl)-D-fructose-hydrolase.[1][2][3][4][5] This enzyme catalyses the following chemical reaction
Hydrolysis of (2->6)-beta-D-fructofuranan, to remove successive disaccharide residues as levanbiose, i.e. 6-(beta-D-fructofuranosyl)-D-fructose, from the end of the chain
References
^Avigad, G.; Zelikson, R. (1963). "Cleavage of fructans to levanbiose by a specific hydrolase". Bull. Res. Counc. Isr. 11: 253–257.
^Saito, K.; Kondo, K.; Kojima, I.; Yokota, A.; Tomita, F. (2000). "Purification and characterization of 2,6-β-D-fructan 6-levanbiohydrolase from Streptomyces exfoliatus F3-2". Appl. Environ. Microbiol. 66: 252–256. doi:10.1128/aem.66.1.252-256.2000. PMID10618232.
^Saito, K.; Oda, Y.; Tomita, F.; Yokota, A. (2003). "Molecular cloning of the gene for 2,6-β-D-fructan 6-levanbiohydrolase from Streptomyces exfoliatus F3-2". FEMS Microbiol. Lett. 218: 265–270. doi:10.1111/j.1574-6968.2003.tb11527.x. PMID12586402.
^Song, E.K.; Kim, H.; Sung, H.K.; Cha, J. (2002). "Cloning and characterization of a levanbiohydrolase from Microbacterium laevaniformans ATCC 15953". Gene. 291: 45–55. doi:10.1016/s0378-1119(02)00630-3. PMID12095678.
^Kang, E.J.; Lee, S.O.; Lee, J.D.; Lee, T.H.; Lee, T.H. (1999). "Purification and characterization of a levanbiose-producing levanase from Pseudomonas sp. No. 43". Biotechnol. Appl. Biochem. 29: 263–268. PMID10334957.
