|PDB structures||RCSB PDB PDBe PDBsum|
Invertase is an enzyme that catalyzes the hydrolysis (breakdown) of sucrose (table sugar) into fructose and glucose. Alternative names for invertase include EC 188.8.131.52, saccharase, glucosucrase, beta-h-fructosidase, beta-fructosidase, invertin, sucrase, maxinvert L 1000, fructosylinvertase, alkaline invertase, acid invertase, and the systematic name: beta-fructofuranosidase. The resulting mixture of fructose and glucose is called inverted sugar syrup. Related to invertases are sucrases. Invertases and sucrases hydrolyze sucrose to give the same mixture of glucose and fructose. Invertases cleave the O-C(fructose) bond, whereas the sucrases cleave the O-C(glucose) bond.
For industrial use, invertase is usually derived from yeast. It is also synthesized by bees, which use it to make honey from nectar. Optimal temperature at which the rate of reaction is at its greatest is 60 °C and an optimum pH of 4.5. Typically, sugar is inverted with sulfuric acid.[dubious ]
Applications and examples
Chocolate-covered cherries, other cordials, and fondant candies include invertase, which liquefies the sugar. Once the candy is manufactured, it needs at least a few days to a few weeks in storage so the invertase has time to break down the sucrose.
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- Chase, Aurin M.; Von Meier, Hildegard C.; Menna, Vincent J. (1962-02). "The Non-Competitive Inhibition and Irreversible Inactivation of Yeast Invertase by Urea". Journal of Cellular and Comparative Physiology. 59 (1): 1–13. doi:10.1002/jcp.1030590102. ISSN 0095-9898. Check date values in:
- Rajagopalan, K. V.; Fridovich, Irwin; Handler, Philip (1961-04-01). "Competitive Inhibition of Enzyme Activity by Urea". Journal of Biological Chemistry. 236 (4): 1059–1065. ISSN 0021-9258. PMID 13739212.