Glycoside hydrolase family 4
| Family 4 glycosyl hydrolase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 Structure of the 6-phospho-beta glucosidase from Thermotoga maritima at 2.55 angstrom resolution in the tetragonal form with manganese, NAD+ and glucose-6-phosphate | |||||||||
| Identifiers | |||||||||
| Symbol | Glyco_hydro_4 | ||||||||
| Pfam | PF02056 | ||||||||
| Pfam clan | CL0063 | ||||||||
| InterPro | IPR001088 | ||||||||
| PROSITE | PDOC01027 | ||||||||
| SCOP2 | 1obb / SCOPe / SUPFAM | ||||||||
| CAZy | GH4 | ||||||||
| |||||||||
| Family 4 glycosyl hydrolase C-terminal domain | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| Symbol | Glyco_hydro_4C | ||||||||
| Pfam | PF11975 | ||||||||
| Pfam clan | CL0341 | ||||||||
| PROSITE | PDOC01027 | ||||||||
| SCOP2 | 1obb / SCOPe / SUPFAM | ||||||||
| CAZy | GH4 | ||||||||
| |||||||||
In molecular biology, glycoside hydrolase family 4 is a family of glycoside hydrolases.
Glycoside hydrolases EC 3.2.1. are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of >100 different families.[1][2][3] This classification is available on the CAZy web site,[4][5] and also discussed at CAZypedia, an online encyclopedia of carbohydrate active enzymes.[6][7]
Glycoside hydrolase family 4 CAZY GH_4 comprises enzymes with several known activities; 6-phospho-beta-glucosidase (EC 3.2.1.86); 6-phospho-alpha-glucosidase (EC 3.2.1.122); alpha-galactosidase (EC 3.2.1.22). 6-phospho-alpha-glucosidase requires both NAD(H) and divalent metal (Mn2+, Fe2+, Co2+, or Ni2+) for activity.[8]
External references
References
- ^ Henrissat B, Callebaut I, Fabrega S, Lehn P, Mornon JP, Davies G (July 1995). "Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases". Proceedings of the National Academy of Sciences of the United States of America. 92 (15): 7090–4. Bibcode:1995PNAS...92.7090H. doi:10.1073/pnas.92.15.7090. PMC 41477. PMID 7624375.
- ^ Davies G, Henrissat B (September 1995). "Structures and mechanisms of glycosyl hydrolases". Structure. 3 (9): 853–9. doi:10.1016/S0969-2126(01)00220-9. PMID 8535779.
- ^ Henrissat B, Bairoch A (June 1996). "Updating the sequence-based classification of glycosyl hydrolases". The Biochemical Journal. 316 (Pt 2): 695–6. doi:10.1042/bj3160695. PMC 1217404. PMID 8687420.
- ^ "Home". CAZy.org. Retrieved 2018-03-06.
- ^ Lombard V, Golaconda Ramulu H, Drula E, Coutinho PM, Henrissat B (January 2014). "The carbohydrate-active enzymes database (CAZy) in 2013". Nucleic Acids Research. 42 (Database issue): D490-5. doi:10.1093/nar/gkt1178. PMC 3965031. PMID 24270786.
- ^ "Glycoside Hydrolase Family 4". CAZypedia.org. Retrieved 2018-03-06.
- ^ CAZypedia Consortium (December 2018). "Ten years of CAZypedia: a living encyclopedia of carbohydrate-active enzymes" (PDF). Glycobiology. 28 (1): 3–8. doi:10.1093/glycob/cwx089. PMID 29040563.
- ^ Thompson J, Pikis A, Ruvinov SB, Henrissat B, Yamamoto H, Sekiguchi J (October 1998). "The gene glvA of Bacillus subtilis 168 encodes a metal-requiring, NAD(H)-dependent 6-phospho-alpha-glucosidase. Assignment to family 4 of the glycosylhydrolase superfamily". The Journal of Biological Chemistry. 273 (42): 27347–56. doi:10.1074/jbc.273.42.27347. PMID 9765262.
