Glycoside hydrolase family 4
| Family 4 glycosyl hydrolase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 structure of the 6-phospho-beta glucosidase from thermotoga maritima at 2.55 angstrom resolution in the tetragonal form with manganese, nad+ and glucose-6-phosphate | |||||||||
| Identifiers | |||||||||
| Symbol | Glyco_hydro_4 | ||||||||
| Pfam | PF02056 | ||||||||
| Pfam clan | CL0063 | ||||||||
| InterPro | IPR001088 | ||||||||
| PROSITE | PDOC01027 | ||||||||
| SCOP2 | 1obb / SCOPe / SUPFAM | ||||||||
| CAZy | GH4 | ||||||||
| |||||||||
| Family 4 glycosyl hydrolase C-terminal domain | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| Symbol | Glyco_hydro_4C | ||||||||
| Pfam | PF11975 | ||||||||
| Pfam clan | CL0341 | ||||||||
| PROSITE | PDOC01027 | ||||||||
| SCOP2 | 1obb / SCOPe / SUPFAM | ||||||||
| CAZy | GH4 | ||||||||
| |||||||||
In molecular biology, glycoside hydrolase family 4 is a family of glycoside hydrolases.
Glycoside hydrolases EC 3.2.1. are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of >100 different families.[1][2][3] This classification is available on the CAZy(http://www.cazy.org/GH1.html) web site,[4] and also discussed at CAZypedia, an online encyclopedia of carbohydrate active enzymes.[5]
Glycoside hydrolase family 4 CAZY GH_4 comprises enzymes with several known activities; 6-phospho-beta-glucosidase (EC 3.2.1.86); 6-phospho-alpha-glucosidase (EC 3.2.1.122); alpha-galactosidase (EC 3.2.1.22). 6-phospho-alpha-glucosidase requires both NAD(H) and divalent metal (Mn2+, Fe2+, Co2+, or Ni2+) for activity.[6]
External references
References
- ^ Henrissat B, Callebaut I, Mornon JP, Fabrega S, Lehn P, Davies G (1995). "Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases". Proc. Natl. Acad. Sci. U.S.A. 92 (15): 7090–7094. doi:10.1073/pnas.92.15.7090. PMC 41477. PMID 7624375.
- ^ Henrissat B, Davies G (1995). "Structures and mechanisms of glycosyl hydrolases". Structure. 3 (9): 853–859. doi:10.1016/S0969-2126(01)00220-9. PMID 8535779.
- ^ Bairoch, A. "Classification of glycosyl hydrolase families and index of glycosyl hydrolase entries in SWISS-PROT". 1999.
- ^ Henrissat, B. and Coutinho P.M. "Carbohydrate-Active Enzymes server". 1999.
- ^ CAZypedia, an online encyclopedia of carbohydrate-active enzymes.
- ^ Henrissat B, Thompson J, Yamamoto H, Pikis A, Ruvinov SB, Sekiguchi J (1998). "The gene glvA of Bacillus subtilis 168 encodes a metal-requiring, NAD(H)-dependent 6-phospho-alpha-glucosidase. Assignment to family 4 of the glycosylhydrolase superfamily". J. Biol. Chem. 273 (42): 27347–27356. doi:10.1074/jbc.273.42.27347. PMID 9765262.
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