Glycoside hydrolase family 15
|Glycosyl hydrolases family 15|
Glycoside hydrolases EC 3.2.1. are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of >100 different families. This classification is available on the CAZy web site, and also discussed at CAZypedia, an online encyclopedia of carbohydrate active enzymes.y[ _]9
Glucoamylase (GA) catalyses the release of D-glucose from the non-reducing ends of starch and other oligo- or poly-saccharides. Studies of fungal GA have indicated 3 closely clustered acidic residues that play a role in the catalytic mechanism. This region is also conserved in a recently sequenced bacterial GA.
The 3D structure of the pseudo-tetrasaccharide acarbose complexed with glucoamylase II(471) from Aspergillus awamori var. X100 has been determined to 2.4A resolution. The protein belongs to the mainly alpha class, and contains 19 helices and 9 strands.
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- Aleshin AE, Firsov LM, Honzatko RB (June 1994). "Refined structure for the complex of acarbose with glucoamylase from Aspergillus awamori var. X100 to 2.4-A resolution". The Journal of Biological Chemistry. 269 (22): 15631–9. PMID 8195212.