Glycoside hydrolase family 47

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Glycosyl hydrolase family 47
Identifiers
Symbol Glyco_hydro_47
Pfam PF01532
Pfam clan CL0059
SCOP 1dl2
SUPERFAMILY 1dl2
CAZy GH47

In molecular biology, glycoside hydrolase family 47 is a family of glycoside hydrolases.

Glycoside hydrolases EC 3.2.1. are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of >100 different families.[1][2][3] This classification is available on the CAZy(http://www.cazy.org/GH1.html) web site,[4] and also discussed at CAZypedia, an online encyclopedia of carbohydrate active enzymes.[5]

Glycoside hydrolase family 47 CAZY GH_47 comprises enzymes with only one known activity; alpha-mannosidase (EC 3.2.1.113).

Alpha-mannosidase is involved in the maturation of Asn-linked oligosaccharides.[6] The enzyme hydrolyses terminal 1,2-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide man(9)(glcnac)(2) in a calcium-dependent manner. The mannose residues are trimmed away to produce, first, man(8)glcnac(2), then a man(5)(glcnac)(2) structure.

References[edit]

  1. ^ Henrissat B, Callebaut I, Mornon JP, Fabrega S, Lehn P, Davies G (1995). "Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases". Proc. Natl. Acad. Sci. U.S.A. 92 (15): 7090–7094. PMC 41477Freely accessible. PMID 7624375. doi:10.1073/pnas.92.15.7090. 
  2. ^ Henrissat B, Davies G (1995). "Structures and mechanisms of glycosyl hydrolases". Structure. 3 (9): 853–859. PMID 8535779. doi:10.1016/S0969-2126(01)00220-9. 
  3. ^ Bairoch, A. "Classification of glycosyl hydrolase families and index of glycosyl hydrolase entries in SWISS-PROT". 1999.
  4. ^ Henrissat, B. and Coutinho P.M. "Carbohydrate-Active Enzymes server". 1999.
  5. ^ CAZypedia, an online encyclopedia of carbohydrate-active enzymes.
  6. ^ Neame PJ, Lal A, Schutzbach JS, Forsee WT, Moremen KW (1994). "Isolation and expression of murine and rabbit cDNAs encoding an alpha 1,2-mannosidase involved in the processing of asparagine-linked oligosaccharides". J. Biol. Chem. 269 (13): 9872–9881. PMID 8144580.