Royal jelly is a honey bee secretion that is used in the nutrition of larvae and adult queens. It is secreted from the glands in the hypopharynx of nurse bees, and fed to all larvae in the colony, regardless of sex or caste.
During the process of creating new queens, the workers construct special queen cells. The larvae in these cells are fed with copious amounts of royal jelly. This type of feeding triggers the development of queen morphology, including the fully developed ovaries needed to lay eggs.
Royal jelly is sometimes used in alternative medicine under the category apitherapy. Studies have suggested that royal jelly is likely to enhance collagen production, vasodilation, in reducing premenstrual syndrome effects, and as a postmenopausal treatment in humans, and against Alzheimer's disease in animals. It is often sold as a dietary supplement for humans, but the European Food Safety Authority has concluded that current evidence does not support the claim that consuming royal jelly offers health benefits to humans. In the United States, the Food and Drug Administration has taken legal action against companies that have marketed royal jelly products using unfounded claims of health benefits.
Royal jelly is secreted from the glands in the heads of worker bees and is fed to all bee larvae, whether they are destined to become drones (males), workers (sterile females), or queens (fertile females). After three days, the drone and worker larvae are no longer fed with royal jelly, but queen larvae continue to be fed this special substance throughout their development.
Royal jelly is 67% water, 12.5% protein, 11% simple sugars (monosaccharides), 6% fatty acids and 3.5% 10-hydroxy-2-decenoic acid (10-HDA). It also contains trace minerals, antibacterial and antibiotic components, pantothenic acid (vitamin B5), pyridoxine (vitamin B6) and trace amounts of vitamin C, but none of the fat-soluble vitamins: A, D, E or K.
Major royal jelly proteins (MRJPs) are a family of proteins secreted by honey bees. The family consists of nine proteins, of which MRJP1 (also called royalactin), MRJP2, MRJP3, MRJP4, and MRJP5 are present in the royal jelly secreted by worker bees. MRJP1 is the most abundant, and largest in size. The five proteins constitute 83–90% of the total proteins in royal jelly. Royal jelly has been used in traditional medicine since ancient times, and the MRJPs are shown to be the main medicinal components. They are synthesised by a family of nine genes (mrjp genes), which are in turn members of the yellow family of genes such as in the fruitfly (Drosophila) and bacteria. They are attributed to be involved in differential development of queen larva and worker larvae, thus establishing division of labour in the bee colony.
The honey bee queens and workers represent one of the most striking examples of environmentally controlled phenotypic polymorphism. Even if two larvae had identical DNA, one raised to be a worker, the other a queen, the two adults would be strongly differentiated across a wide range of characteristics including anatomical and physiological differences, longevity, and reproductive capacity. Queens constitute the female sexual caste and have large active ovaries, whereas female workers have only rudimentary, inactive ovaries and are functionally sterile. The queen–worker developmental divide is controlled epigenetically by differential feeding with royal jelly; this appears to be due specifically to the protein royalactin. A female larva destined to become a queen is fed large quantities of royal jelly; this triggers a cascade of molecular events resulting in development of a queen. It has been shown that this phenomenon is mediated by an epigenetic modification of DNA known as CpG methylation. Silencing the expression of an enzyme that methylates DNA in newly hatched larvae led to a royal jelly-like effect on the larval developmental trajectory; the majority of individuals with reduced DNA methylation levels emerged as queens with fully developed ovaries. This finding suggests that DNA methylation in honey bees allows the expression of epigenetic information to be differentially altered by nutritional input.
Use by humans
Royal jelly is harvested by stimulating colonies with movable frame hives to produce queen bees. Royal jelly is collected from each individual queen cell (honeycomb) when the queen larvae are about four days old. These are the only cells in which large amounts are deposited; when royal jelly is fed to worker larvae, it is fed directly to them, and they consume it as it is produced, while the cells of queen larvae are "stocked" with royal jelly much faster than the larvae can consume it. Therefore, only in queen cells is the harvest of royal jelly practical. A well-managed hive during a season of 5–6 months can produce approximately 500 g of royal jelly. Since the product is perishable, producers must have immediate access to proper cold storage (e.g., a household refrigerator or freezer) in which the royal jelly is stored until it is sold or conveyed to a collection center. Sometimes honey or beeswax is added to the royal jelly, which is thought to aid its preservation.
Royal jelly may cause allergic reactions in humans ranging from hives, asthma, to even fatal anaphylaxis. The incidence of allergic side effects in people who consume royal jelly is unknown. The risk of having an allergy to royal jelly is higher in people who have other allergies.
- 3-Hydroxydecanoic acid
- 3,10-Dihydroxydecanoic acid
- 3,11-Dihydroxydodecanoic acid
- Bee propolis
- Queen bee acid
- Jung-Hoffmann, L (1966). "Die Determination von Königin und Arbeiterin der Honigbiene". Z Bienenforsch. 8: 296–322.
- Graham, J. (ed.) (1992) The Hive and the Honey Bee (Revised Edition). Dadant & Sons.[page needed]
- Maleszka, Ryszard (27 October 2014). "Epigenetic integration of environmental and genomic signals in honey bees: the critical interplay of nutritional, brain and reproductive networks". Epigenetics. 3 (4): 188–192. doi:10.4161/epi.3.4.6697. PMID 18719401.
- Park, Hye Min; Hwang, Eunson; Lee, Kwang Gill; Han, Sang-Mi; Cho, Yunhi; Kim, Sun Yeou (September 2011). "Royal Jelly Protects Against Ultraviolet B–Induced Photoaging in Human Skin Fibroblasts via Enhancing Collagen Production". Journal of Medicinal Food. 14 (9): 899–906. doi:10.1089/jmf.2010.1363. PMID 21812645.
- Siavash, Mansour; Shokri, Saeideh; Haghighi, Sepehr; Shahtalebi, Mohammad Ali; Farajzadehgan, Ziba (April 2015). "The efficacy of topical royal jelly on healing of diabetic foot ulcers: a double-blind placebo-controlled clinical trial". International Wound Journal. 12 (2): 137–142. doi:10.1111/iwj.12063. PMC 7950674. PMID 23566071.
- Taavoni, Simin; Barkhordari, Fatemeh; Goushegir, Ashrafeddin; Haghani, Hamid (1 August 2014). "Effect of Royal Jelly on premenstrual syndrome among Iranian medical sciences students: A randomized, triple-blind, placebo-controlled study". Complementary Therapies in Medicine. 22 (4): 601–606. doi:10.1016/j.ctim.2014.05.004. PMID 25146061.
- Seyyedi, Fatemeh (2016). "Comparison of the Effects of Vaginal Royal Jelly and Vaginal Estrogen on Quality of Life, Sexual and Urinary Function in Postmenopausal Women". Journal of Clinical and Diagnostic Research. 10 (5): QC01-5. doi:10.7860/JCDR/2016/17844.7715. PMC 4948482. PMID 27437306.
- Reisi, Parham; Pilehvarian, AliAsghar; Zamani, Zohre; Alaei, Hojjatallah (2012). "Effect of Royal Jelly on spatial learning and memory in rat model of streptozotocin-induced sporadic Alzheimer′s disease". Advanced Biomedical Research. 1 (1): 26. doi:10.4103/2277-9175.98150. PMC 3507025. PMID 23210085.
- "Scientific Opinion on the substantiation of health claims related to: anthocyanidins and proanthocyanidins (ID 1787, 1788, 1789, 1790, 1791); sodium alginate and ulva (ID 1873); vitamins, minerals, trace elements and standardised ginseng G115 extract (ID". EFSA Journal. 9 (4): 2083. April 2011. doi:10.2903/j.efsa.2011.2083.
- "Federal Government Seizes Dozens of Misbranded Drug Products: FDA warned company about making medical claims for bee-derived products". Food and Drug Administration. Apr 5, 2010.
- "Inspections, Compliance, Enforcement, and Criminal Investigations: Beehive Botanicals, Inc". Food and Drug Administration. March 2, 2007.
- "Value-added products from beekeeping. Chapter 6".
- Buttstedt, Anja; Moritz, Robin F. A.; Erler, Silvio (May 2014). "Origin and function of the major royal jelly proteins of the honeybee (Apis mellifera) as members of the yellow gene family". Biological Reviews. 89 (2): 255–269. doi:10.1111/brv.12052. PMID 23855350. S2CID 29361726.
- Albert, Stefan; Bhattacharya, Debashish; Klaudiny, Jaroslav; Schmitzová, Jana; Simúth, Jozef (August 1999). "The Family of Major Royal Jelly Proteins and Its Evolution". Journal of Molecular Evolution. 49 (2): 290–297. Bibcode:1999JMolE..49..290A. doi:10.1007/pl00006551. PMID 10441680. S2CID 27316541.
- Winston, M, The Biology of the Honey Bee, 1987, Harvard University Press[page needed]
- Kucharski, R.; Maleszka, J.; Foret, S.; Maleszka, R. (13 March 2008). "Nutritional Control of Reproductive Status in Honeybees via DNA Methylation". Science. 319 (5871): 1827–1830. Bibcode:2008Sci...319.1827K. doi:10.1126/science.1153069. PMID 18339900. S2CID 955740.
- Kucharski, R.; Maleszka, J.; Foret, S.; Maleszka, R. (2008). "Nutritional Control of Reproductive Status in Honeybees via DNA Methylation". Science. 319 (5871): 1827–1830. Bibcode:2008Sci...319.1827K. doi:10.1126/science.1153069. PMID 18339900. S2CID 955740.
- Leung, R; Ho, A; Chan, J; Choy, D; Lai, CK (March 1997). "Royal jelly consumption and hypersensitivity in the community". Clin. Exp. Allergy. 27 (3): 333–6. doi:10.1111/j.1365-2222.1997.tb00712.x. PMID 9088660. S2CID 19626487.
- Takahama H, Shimazu T (2006). "Food-induced anaphylaxis caused by ingestion of royal jelly". J. Dermatol. 33 (6): 424–426. doi:10.1111/j.1346-8138.2006.00100.x. PMID 16700835. S2CID 37561982.
- Lombardi C, Senna GE, Gatti B, Feligioni M, Riva G, Bonadonna P, Dama AR, Canonica GW, Passalacqua G (1998). "Allergic reactions to honey and royal jelly and their relationship with sensitization to compositae". Allergol. Immunopathol. 26 (6): 288–290.
- Thien FC, Leung R, Baldo BA, Weiner JA, Plomley R, Czarny D (1996). "Asthma and anaphylaxis induced by royal jelly". Clin. Exp. Allergy. 26 (2): 216–222. doi:10.1111/j.1365-2222.1996.tb00082.x. PMID 8835130. S2CID 12422547.
- Leung R, Thien FC, Baldo B, Czarny D (1995). "Royal jelly-induced asthma and anaphylaxis: clinical characteristics and immunologic correlations". J. Allergy Clin. Immunol. 96 (6 Pt 1): 1004–1007. doi:10.1016/S0091-6749(95)70242-3. PMID 8543734.
- Bullock RJ, Rohan A, Straatmans JA (1994). "Fatal royal jelly-induced asthma". Med. J. Aust. 160 (1): 44. doi:10.5694/j.1326-5377.1994.tb138207.x. PMID 8271989. S2CID 37201999.
- Balch, Phyllis A.; Balch, James F. (2000). Prescription for Nutritional Healing, Third Edition. New York: Avery. ISBN 978-1-58333-077-7.
- Ammon, R.; Zoch, E. (1957). "Zur Biochemie des Futtersaftes der Bienenkoenigin". Arzneimittel-Forschung. 7: 699–702.
- Blum, M.S.; Novak, A.F.; Taber, S. (1959). "10-Hydroxydecenoic Acid, an antibiotic found in royal jelly". Science. 130 (3373): 452–453. doi:10.1126/science.130.3373.452. PMID 13675771. S2CID 83635354.
- Bonomi, A (1983). "Acquisizioni in tema di composizione chimica e di attivita' biologica della pappa reale". Apitalia. 10 (15): 7–13.
- Braines, L.N. (1959). Royal jelly I. Inform. Bull. Inst. Pchelovodstva, 31 pp (with various articles)
- Braines, L.N. (1960). Royal jelly II. Inform. Bull. Inst. Pchelovodstva, 40 pp.
- Braines, L.N. (1962). Royal jelly III. Inform. Bull. Inst. Pchelovodstva, 40
- Chauvin, R. and Louveaux, 1. (1956) Etdue macroscopique et microscopique de lagelee royale. L'apiculteur.
- Cho, Y.T. (1977). "Studies on royal jelly and abnormal cholesterol and triglycerides". Amer. Bee. 117: 36–38.
- De Belfever, B. (1958) La gelee royale des abeilles. Maloine, Paris.
- Destrem, H. (1956) Experimentation de la gelee royale d'abeille en pratique geriatrique (134 cas). Rev. Franc. Geront, 3.
- Giordani, G (1961). "[Effect of royal jelly on chickens.]". Avicoltura. 30 (6): 114–120.
- Hattori N, Nomoto H, Fukumitsu H, Mishima S, Furukawa S. [Royal jelly and its unique fatty acid, 10-hydroxy-trans-2-decenoic acid, promote neurogenesis by neural stem/progenitor cells in vitro.] Biomed Res. 2007 Oct;28(5):261-6.
- Hashimoto, M; Kanda, M; Ikeno, K; Hayashi, Y; Nakamura, T; Ogawa, Y; Fukumitsu, H; Nomoto, H; Furukawa, S (Apr 2005). "Oral administration of royal jelly facilitates mRNA expression of glial cell line-derived neurotrophic factor and neurofilament H in the hippocampus of the adult mouse brain". Biosci. Biotechnol. Biochem. 69 (4): 800–5. doi:10.1271/bbb.69.800. PMID 15849420. S2CID 14746946.
- Inoue, T. (1986). The use and utilization of royal jelly and the evaluation of the medical efficacy of royal jelly in Japan. Proceeding sof the XXXth International Congress of Apiculture, Nagoya, 1985, Apimondia, 444-447
- Jean, E (1956). "A process of royal jelly absorption for its incorporation into assimilable substances". Fr. Pat. 1 (118): 123.
- Jacoli, G (1956). "Ricerche sperimentali su alcune proprieta' biologiche della gelatina reale". Apicoltore d'Italia. 23 (9–10): 211–214.
- Jung-Hoffmann, L (1966). "Die Determination von Königin und Arbeiterin der Honigbiene". Z. Bienenforsch. 8: 296–322.
- Karaali, A.; Meydanoglu, F.; Eke, D. (1988). "Studies on composition, freeze drying and storage of Turkish royal jelly". J. Apic. Res. 27 (3): 182–185. doi:10.1080/00218839.1988.11100799.
- Kucharski R, Maleszka, J, Foret, S, Maleszka, R, Nutritional Control of Reproductive Status in Honeybees via DNA Methylation. Science. 2008 Mar 28;319(5871):1827-3
- Lercker, G.; Capella, P.; Conte, L.S.; Ruini, F.; Giordani, G. (1982). "Components of royal jelly: II. The lipid fraction, hydrocarbons and sterolds". J. Apic. Res. 21 (3): 178–184. doi:10.1080/00218839.1982.11100538.
- Lercker, G.; Vecchi, M.A.; Sabatini, A.G.; Nanetti, A. (1984). "Controllo chimicoanalitico della gelatina reale". Riv. Merceol. 23 (1): 83–94.
- Lercker, G.; Savioli, S.; Vecchi, M.A.; Sabatini, A.G.; Nanetti, A.; Piana, L. (1986). "Carbohydrate Determination of Royal Jelly by Gas-liquid chromatography–High Resolution Gas Chromatography (HRGC)". Food Chemistry. 19 (4): 255–264. doi:10.1016/0308-8146(86)90049-x.
- Lercker, G.; Caboni, M.F.; Vecchi, M.A.; Sabatini, A.G.; Nanetti, A. (1992). "Caratterizzazione dei principali costituenti della gelatina reale". Apicoltura. 8: 11–21.
- Maleszka, R (2008). "Epigenetic integration of environmental and genomic signals in honey bees: the critical interplay of nutritional, brain and reproductive networks". Epigenetics. 3 (4): 188–192. doi:10.4161/epi.3.4.6697. PMID 18719401.
- Nakamura, T. (1986) Quality standards of royal jelly for medical use. proceedings of the XXXth International Congress of Apiculture, Nagoya, 1985 Apimondia (1986) 462–464.
- Rembold, H (1965). Biologically active substances in royal jelly. Vitamins & Hormones. 23. pp. 359–382. doi:10.1016/S0083-6729(08)60385-4. ISBN 9780127098234. PMID 5326344.
- Salama, A.; Mogawer, H.H.; El-Tohamy, M. (1977). "Royal jelly a revelation or a fable". Egyptian Journal of Veterinary Science. 14 (2): 95–102.
- Takenaka, T. Nitrogen components and carboxylic acids of royal jelly. In Chemistry and biology of social insects (edited by Eder, J., Rembold, H.). Munich, German Federal Republic, Verlag J. Papemy (1987): 162–163.
- Wagner, H.; Dobler, I.; Thiem, I. (1970). "Effect of royal jelly on the peirpheral blood and survival rate of mice after irradiation of the entire body with X-rays". Radiobiologia Radiotherapia. 11 (3): 323–328.
- Winston, M, The Biology of the Honey Bee, 1987, Harvard University Press
- Media related to Royal jelly at Wikimedia Commons