Pwo DNA polymerase

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Structures	Swiss-model
Domains	InterPro

DNA polymerase
DNA polymerase
Identifiers
Organism	Pyrococcus woesei
Symbol	pol
UniProt	P61876
Structures
Search for
Structures	Swiss-model
Domains	InterPro

Pwo polymerase is a thermostable DNA polymerase used for the polymerase chain reaction. The abbreviation stands for Pyrococcus woesei, a thermophilic archaeon, from which this polymerase was isolated. This polymerase breaks when reaching erroneous uracil in DNA from the chain extension and, through this readahead function, fewer defective DNA clones are synthesized. It is used much less than the usual Taq or Pfu polymerases.^[1]

References

^ Ghasemi A, Salmanian AH, Sadeghifard N, Salarian AA, Gholi MK (September 2011). "Cloning, expression and purification of Pwo polymerase from Pyrococcus woesei". Iranian Journal of Microbiology. 3 (3): 118–22. PMC 3279813. PMID 22347593.

[1] Ghasemi A, Salmanian AH, Sadeghifard N, Salarian AA, Gholi MK (September 2011). "Cloning, expression and purification of Pwo polymerase from Pyrococcus woesei". Iranian Journal of Microbiology. 3 (3): 118–22. PMC 3279813. PMID 22347593.

[1]

v t e Polymerase chain reaction techniques
Procedure	Initialization Cycle Denaturation Annealing Elongation Final elongation Final hold
Polymerase	Klenow T4 and T7 Taq Tth Pfu Vent Pwo Tli Tfu
Optimization and variants	Quantitative polymerase chain reaction (qPCR) Reverse transcription polymerase chain reaction (RT-PCR) Inverse polymerase chain reaction (inverse PCR) Nested polymerase chain reaction (nested PCR) Touchdown polymerase chain reaction Hot start PCR Overlap extension polymerase chain reaction (OE-PCR) Multiplex polymerase chain reaction (multiplex PCR) Multiplex ligation-dependent probe amplification (MLPA) Co-amplification at lower denaturation temperature PCR (COLD-PCR) Digital polymerase chain reaction (dePCR) Random amplification of polymorphic DNA (RAPD)
History and people	Kary Mullis Kjell Kleppe Alice Chien