Expanded genetic code
An expanded genetic code is an artificially modified genetic code in which one or more specific codons have been re-allocated to encode an amino acid that is not among the 20 common naturally-encoded proteinogenic amino acids.
The key prerequisites to expand the genetic code are:
- the non-standard amino acid to encode,
- an unused codon to adopt,
- a tRNA that recognises this codon, and
- a tRNA synthetase that recognises only that tRNA and only the non-standard amino acid.
Expanding the genetic code is an area of research of synthetic biology, an applied biological discipline whose goal is to engineer living systems for useful purposes. The genetic code expansion enriches the repertoire of useful tools available to science.
- 1 Introduction
- 2 Non-standard amino acids
- 3 Codon assignment
- 4 tRNA/synthetase pair
- 5 Orthogonal Ribosomes
- 6 Applications
- 7 Future
- 8 Related methods
- 9 See also
- 10 References
It is noteworthy that the genetic code for all organisms is basically the same, so that all living beings use the same ’genetic language’. In general, the introduction of new functional unnatural amino acids into proteins of living cells breaks the universality of the genetic language, which ideally leads to alternative life forms. Proteins are produced thanks to the translational system molecules, which decode the RNA messages into a string of amino acids. The translation of genetic information contained in messenger RNA (mRNA) into a protein is catalysed by ribosomes. Transfer RNAs (tRNA) are used as keys to decode the mRNA into its encoded polypeptide. The tRNA recognizes a specific three nucleotide codon in the mRNA with a complementary sequence called the anticodon on one of its loops. Each three nucleotide codon is translated into one of twenty naturally occurring amino acids. There is at least one tRNA for any codon, and sometimes multiple codons code for the same amino acid. Many tRNAs are compatible with several codons. An enzyme called an aminoacyl tRNA synthetase covalently attaches the amino acid to the appropriate tRNA. Most cells have a different synthetase for each amino acid (20 or more synthetases). On the other hand, some bacteria have fewer than 20 aminoacyl tRNA synthetases, and introduce the "missing" amino acid(s) by modification of a structurally related amino acid by an aminotransferase enzyme. A feature exploited in the expansion of the genetic code is the fact that the aminoacyl tRNA synthetase often does not recognize the anticodon, but another part of the tRNA, meaning that if the anticodon were to be mutated the encoding of that amino acid would change to a new codon. In the ribosome, the information in mRNA is translated into a specific amino acid when the mRNA codon matches with the complementary anticodon of a tRNA, and the attached amino acid is added onto a growing polypeptide chain. When it is released from the ribosome, the polypeptide chain folds into a functioning protein.
In order to incorporate a novel amino acid into the genetic code several changes are required. First, for successful translation of a novel amino acid, the codon to which the novel amino acid is assigned cannot already code for one of the 20 natural amino acids. Usually a nonsense codon (stop codon) or a four-base codon are used. Second, a novel pair of tRNA and aminoacyl tRNA synthetase are required, these are called the orthogonal set. The orthogonal set must not crosstalk with the endogenous tRNA and synthetase sets, while still being functionally compatible with the ribosome and other components of the translation apparatus. The active site of the synthetase is modified to accept only the novel amino acid. Most often, a library of mutant synthetases is screened for one which charges the tRNA with the desired amino acid. The synthetase is also modified to recognize only the orthogonal tRNA. The tRNA synthetase pair is often engineered in other bacteria or eukaryotic cells.
In this area of research, the 20 encoded proteinogenic amino acids are referred to as standard amino acids, or alternatively as natural or canonical amino acids, while the added amino acids are called non-standard amino acids (NSAAs), or unnatural amino acids (uAAs; term not used in papers dealing with natural non-proteinogenic amino acids, such as phosphoserine), or non-canonical amino acids.
Non-standard amino acids
The first element of the system is the amino acid that is added to the genetic code of a certain strain of organism.
Over 71 different NSAAs have been added to different strains of E. coli, yeast or mammalian cells. Due to technical details (easier chemical synthesis of NSAAs, less crosstalk and easier evolution of the aminoacyl-tRNA synthase), the NSAAs are generally larger than standard amino acids and most often have a phenylalanine core but with a large variety of different substituents. These allow a large repertoire of new functions, such as labelling (see figure), as a fluorescent reporter (e.g. dansylalanine) or to produce translationally protein in E. coli with Eukaryotic post-translational modifications (e.g. phosphoserine, phosphothreonine, and phosphotyrosine).
Unnatural amino acids incorporated into proteins include heavy atom containing amino acids to facilitate certain x-ray crystallographic studies; amino acids with novel steric/packing and electronic properties; photocrosslinking amino acids which can be used to probe protein-protein interactions in vitro or in vivo; keto, acetylene, azide, and boronate containing amino acids which can be used to selectively introduce a large number of biophysical probes, tags, and novel chemical functional groups into proteins in vitro or in vivo; redox active amino acids to probe and modulate electron transfer; photocaged and photoisomerizable amino acids to photoregulate biological processes; metal binding amino acids for catalysis and metal ion sensing; amino acids that contain fluorescent or infra-red active side chains to probe protein structure and dynamics; α-hydroxy acids and D-amino acids as probes of backbone conformation and hydrogen bonding interactions; and sulfated amino acids and mimetics of phosphorylated amino acids as probes of posttranslational modifications.
Availability of the non-standard amino acid requires that the organism either import it from the medium or biosynthesised it. In the first case, the unnatural amino acid is first synthesised chemically in optically pure L-form. It is then added to the growth medium of the cell. Generally a library of compounds is tested to see which can be imported and incorporated, but often the various transport systems can handle unnatural amino acids with apolar side-chains. In the second case, a biosynthetic paths need to be engineered. One example is an E. coli strain that biosynthesizes a novel, previously unnatural amino acid (p-aminophenylalanine) from basic carbon sources and includes this amino acid in its genetic code. Another example is the production of phosphoserine, which is a natural metabolite and as a consequence the pathway flux had to be altered to increase its production.
Another element of the system is a codon to allocate to the new amino acid.
A major problem for the genetic code expansion is that there are no free codons. The genetic code has a nonrandom layout that shows tell-tale signs of various phases of primordial evolution, however, it has since frozen into place and is near-universally conserved. Nevertheless, some codons are rarer than others. In fact, in E. coli (and all organisms) the codon usage is not equal, but presents several rare codons (see table), the rarest being the amber stop codon (UAG).
|Codon||Amino acid||% abundance|
Amber codon suppression
The possibility of reassigning codons was realized by Normanly et al. in 1990, when a viable mutant strain of E. coli read through the UAG ("amber") stop codon. This was possible thanks to the rarity of this codon and the fact that release factor 1 alone makes the amber codon terminate translation. Later, in the Schultz lab, the tRNATyr/tyrosyl-tRNA synthetase (TyrRS) from Methanococcus jannaschii, an archaebacterium, was used to introduce a tyrosine instead of STOP, the default value of the amber codon. This was possible because of the differences between the endogenous bacterial synthases and the orthologous archaeal synthase, which do not recognize each other. Subsequently, the group evolved the orthologonal tRNA/synthase pair to utilise the non-standard amino acid O-methyltyrosine. This was followed by the larger naphthylalanine and the photocrosslinking benzoylphenylalanine, which proved the potential utility of the system.
The amber codon is the least used codon in Escherichia coli, but hijacking it results in a substantial loss of fitness. One study in fact found that there were at least 83 peptides majorly affected by the readthrough Additionally, the labelling was incomplete. As a consequence, several strains have been made to reduce the fitness cost, including the removal of all amber codons from the genome. In most E. coli K-12 strains (viz. Escherichia coli (molecular biology) for strain pedigrees) there are 314 UAG stop codons. Consequently, a gargantuan amount of work has gone into the replacement of these. One approach pioneered by the group of Prof. George Church from Harvard, was dubbed MAGE in CAGE: this relied on a multiplex transformation and subsequent strain recombination to remove all UAG codons—the latter part presented a halting point in a first paper, but was overcome. This resulted in the E. coli strain C321.ΔA, which lacks all UAG codons and RF1. This allowed an experiment to be done with this strain to make it "addicted" to the amino acid biphenylalanine by evolving several key enzymes to require it structurally, therefore putting its expanded genetic code under positive selection.
Rare sense codon reassignment
In addition to the amber codon, rare sense codons have also been considered for use. The AGG codon codes for arginine, but a strain has been successfully modified to make it code for 6-N-allyloxycarbonyl-lysine. Another candidate is the AUA codon, which is unusual in that its respective tRNA has to differentiate against AUG that codes for methionine (primordially, isoleucine, hence its location). In order to do this, the AUA tRNA has a special base, lysidine. The deletion of the synthase (tilS) was possible thanks to the replacement of the native tRNA with that of Mycoplasma mobile (no lysidine). The reduced fitness is a first step towards pressuring the strain to loose all instances of AUA, allowing it to be used for genetic code expansion.
Four base codons
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Other approaches include the addition of extra base pairing or the use of orthologous ribosomes that accept in addition to the regular triplet genetic code, tRNAs with quadruple code. This allowed the simultaneous usage of two unnatural amino acids, p-azidophenylalanine (pAzF) and N6-[(2-propynyloxy)carbonyl]lysine (CAK), which cross-link with each other by Huisgen cycloaddition.
Another key element is the tRNA/synthetase pair.
The orthologous set of synthetase and tRNA can be mutated and screened through directed evolution to charge the tRNA with a different, even novel, amino acid. Mutations to the plasmid containing the pair can be introduced by error-prone PCR or through degenerate primers for the synthetase's active site. Selection involves multiple rounds of a two-step process, where the plasmid is transferred into cells expressing chloramphenicol acetyl transferase with a premature amber codon. In the presence of toxic chloramphenicol and the non-natural amino acid, the surviving cells will have overridden the amber codon using the orthogonal tRNA aminoacylated with either the standard amino acids or the non-natural one. To remove the former, the plasmid is inserted into cells with a barnase gene (toxic) with a premature amber codon but without the non-natural amino acid, removing all the orthogonal synthases that do not specifically recognize the non-natural amino acid. In addition to the recoding of the tRNA to a different codon, they can be mutated to recognize a four-base codon, allowing additional free coding options. The non-natural amino acid, as a result, introduces diverse physicochemical and biological properties in order to be used as a tool to explore protein structure and function or to create novel or enhanced protein for practical purposes.
Orthogonal sets in E. coli
The orthogonal pairs of synthetase and tRNA that work for one organism may not work for another, as the synthetase may mis-aminoacylate endogenous tRNAs or the tRNA be mis-aminoacylated itself by an endogenous synthetase. As a result, the sets created to date differ between organisms.
- tRNATyr-TyrRS pair from the archaeon Methanococcus jannaschii
- tRNALys–LysRS pair from the archaeon Pyrococcus horikoshii
- tRNAGlu–GluRS pair from Methanosarcina mazei
- leucyl-tRNA synthetase from Methanobacterium thermoautotrophicum and a mutant leucyl tRNA derived from Halobacterium sp
- tRNAAmber-PylRS pair from the archaeon Methanosarcina barkeri and Methanosarcina mazei
- tRNAAmber-3-Iodotyrosyl-tRNA synthetase (variant of aminoacyl-tRNA synthetase from Methanocaldococcus jannaschii)
Orthogonal sets in yeast
- tRNATyr-TyrRS pair from Escherichia coli
- tRNALeu–LeuRS pair from Escherichia coli
- tRNAiMet from human and GlnRS from Escherichia coli
- tRNAAmber-PylRS pair from the archaeon Methanosarcina barkeri and Methanosarcina mazei
- tRNAAmber-4,5-dimethoxy-2-nitrobenzyl-cysteinyl-tRNA synthetase
Orthogonal sets in mammalian cells
- tRNATyr-TyrRS pair from Bacillus stearothermophilus
- modified tRNATrp-TrpRS pair from Bacillus subtilis trp
- tRNALeu–LeuRS pair from Escherichia coli
- tRNAAmber-PylRS pair from the archaeon Methanosarcina barkeri and Methanosarcina mazei
- In 2017 a mouse engineered with an extended genetic code that can produce proteins with unnatural amino acids was reported.
Similarly to orthogonal tRNAs and aminoacyl tRNA synthetases (aaRSs), orthogonal ribosomes have been engineered to work in parallel to the natural ribosomes. Orthogonal ribosomes ideally use different mRNA transcripts than their natural counterparts and ultimately should draw on a separate pool of tRNA as well. This should alleviate some of the loss of fitness which currently still arises from techniques such as Amber codon suppression. Additionally, orthogonal ribosomes can be mutated and optimized for particular tasks, like the recognition of quadruplet codons. Such an optimization is not possible, or highly disadvantageous for natural ribosomes.
In 2005 three sets of ribosomes were published, which did not recognize natural mRNA, but instead translated a separate pool of orthogonal mRNA (o-mRNA). This was achieved by changing the recognition sequence of the mRNA, the Shine-Dalgarno sequence, and the corresponding recognition sequence in the 16S rRNA of ribosomes, the so-called Anti-Shine-Darlgarno-Sequence. This way the base pairing, which is usually lost if either sequence is mutated, stays available. However the mutations in the 16S rRNA were not limited to the obviously base-pairing nucleotides of the classical Anti-Shine-Darlgarno sequence.
In 2007 the group of Jason W. Chin presented an orthogonal ribosome, which was optimized for Amber codon suppression. The 16S rRNA was mutated in such a way that it bound the release factor RF1 less strongly than the natural ribosome does. This ribosome did not eliminate the problem of lowered cell fitness caused by suppressed stop codons in natural proteins. However through the improved specificity it raised the yields of correctly synthesized target protein significantly (from ~20% to >60% percent for one amber codon to be suppressed and form <1% to >20% for two amber codons).
In 2010 the group of Jason W. Chin presented a further optimized version of the orthogonal ribosome. The Ribo-Q is a 16S rRNA optimized to recognize tRNAs, which have quadruplet anti-codons to recognize quadruplet codons, instead of the natural triplet codons. With this approach the number of possible codons rises from 64 to 256. Even accounting for a variety of stop codons, more than 200 different amino acids could potentially be encoded this way.
The orthogonal ribosomes described above all focus on optimizing the 16S rRNA. Thus far, this optimized 16S rRNA was combined with natural large-subunits to form orthogonal ribosomes. If the 23S rRNA, the main RNA-component of the large ribosomal subunit, is to be optimized as well, it had to be assured, that there was no crosstalk in the assembly of orthogonal and natural ribosomes (see figureX B). To ensure that optimized 23S rRNA would only form into ribosomes with the optimized 16S rRNA, the two rRNAs were combined into one transcript. By inserting the sequence for the 23S rRNA into a loop-region of the 16S rRNA sequence, both subunits still adopt functioning folds. Since the two rRNAs are linked and thus in constant proximity, they preferably bind each other, not other free floating ribosomal subunits.
Engineered peptidyl transferase center
In 2014 it was shown that by altering the peptidyl transferase center of the 23S rRNA, ribosomes could be created which draw on orthogonal pools of tRNA. The 3’ end of tRNAs is universally conserved to be CCA. The two cytidines base pair with two guanines the 23S rRNA to bind the tRNA to the ribosome. This interaction is required for translational fidelity. However, by co-mutating the binding nucleotides in such a way, that they can still base pair, the translational fidelity can be conserved. The 3’-end of the tRNA is mutated from CCA to CGA, while two cytidine nucleotides in the ribosomes A- and P-sites are mutated to guanidine. This leads to ribosomes which do not accept naturally occurring tRNAs as substrates and to tRNAs, which cannot be used as substrate by natural ribosomes.
To use such tRNAs effectively, they would have to be aminoacylated by specific, orthogonal aaRSs. Most naturally occurring aaRSs recognize the 3’-end of their corresponding tRNA. aaRSs for these 3’-mutated tRNAs are not available yet. Thus far, this system has only been shown to work in an in-vitro translation setting where the aminoacylation of the orthogonal tRNA was achieved using so called “flexizymes”. Flexizymes are ribozymes with tRNA-amino-aclylation activity.
With an expanded genetic code, the unnatural amino acid can be genetically directed to any chosen site in the protein of interest. The high efficiency and fidelity of this process allows a better control of the placement of the modification compared to modifying the protein post-translationally, which, in general, will target all amino acids of the same type, such as the thiol group of cysteine and the amino group of lysine. Also, an expanded genetic code allows modifications to be carried out in vivo. The ability to site-specifically direct lab-synthesized chemical moieties into proteins allows many types of studies that would otherwise be extremely difficult, such as:
- Probing protein structure and function: By using amino acids with slightly different size such as O-methyltyrosine or dansylalanine instead of tyrosine, and by inserting genetically coded reporter moieties (color-changing and/or spin-active) into selected protein sites, chemical information about the protein's structure and function can be measured.
- Probing the role of post-translational modifications in protein structure and function: By using amino acids that mimic post-translational modifications such as phosphoserine, biologically active protein can be obtained, and the site-specific nature of the amino acid incorporation can lead to information on how the position, density, and distribution of protein phosphorylation effect protein function.
- Identifying and regulating protein activity: By using photocaged aminoacids, protein function can be "switched" on or off by illuminating the organism.
- Changing the mode of action of a protein: One can start with the gene for a protein that binds a certain sequence of DNA and, by inserting a chemically active amino acid into the binding site, convert it to a protein that cuts the DNA rather than binding it.
- Improving immunogenicity and overcoming self-tolerance: By replacing strategically chosen tyrosines with p-nitro phenylalanine, a tolerated self-protein can be made immunogenic.
- Selective destruction of selected cellular components: using an expanded genetic code, unnatural, destructive chemical moieties (sometimes called "chemical warheads") can be incorporated into proteins that target specific cellular components.
- Producing better protein: the evolution of T7 bacteriophages on a non-evolving E. coli strain that encoded 3-iodotyrosine on the amber codon, resulted in a population fitter than wild-type thanks to the presence of iodotyrosine in its proteome
The expansion of the genetic code is still in its infancy. Current methodology uses only one non-standard amino acid at the time, whereas ideally multiple could be used.
Recoded synthetic genome
One way to achieve the encoding of multiple unnatural amino acids is by rewriting genome synthetically. In 2010, at the cost of $40 million an organism, Mycoplasma laboratorium, was constructed that was controlled by a synthetic genome. Due to the larger genome size this is not possible with E. coli, however several methods are being developed to overcome this, such as the fragmentation of the genome into separate linear chromosomes. In addition to the elimination of the usage of rare codons, the specificity of the system needs to be increased as many tRNA recognise several codons
Expanded genetic alphabet
Another approach is to expand the number of nucleobases to increase the coding capacity.
An unnatural base pair (UBP) is a designed subunit (or nucleobase) of DNA which is created in a laboratory and does not occur in nature. A demonstration of UBPs were achieved in vitro by Ichiro Hirao's group at RIKEN institute in Japan. In 2002, they developed an unnatural base pair between 2-amino-8-(2-thienyl)purine (s) and pyridine-2-one (y) that functions in vitro in transcription and translation for the site-specific incorporation of non-standard amino acids into proteins. In 2006, they created 7-(2-thienyl)imidazo[4,5-b]pyridine (Ds) and pyrrole-2-carbaldehyde (Pa) as a third base pair for replication and transcription. Afterward, Ds and 4-[3-(6-aminohexanamido)-1-propynyl]-2-nitropyrrole (Px) was discovered as a high fidelity pair in PCR amplification. In 2013, they applied the Ds-Px pair to DNA aptamer generation by in vitro selection (SELEX) and demonstrated the genetic alphabet expansion significantly augment DNA aptamer affinities to target proteins.
In 2012, a group of American scientists led by Floyd Romesberg, a chemical biologist at the Scripps Research Institute in San Diego, California, published that his team designed an unnatural base pair (UBP). The two new artificial nucleotides or Unnatural Base Pair (UBP) were named "d5SICS" and "dNaM." More technically, these artificial nucleotides bearing hydrophobic nucleobases, feature two fused aromatic rings that form a (d5SICS–dNaM) complex or base pair in DNA. In 2014 the same team from the Scripps Research Institute reported that they synthesized a stretch of circular DNA known as a plasmid containing natural T-A and C-G base pairs along with the best-performing UBP Romesberg's laboratory had designed, and inserted it into cells of the common bacterium E. coli that successfully replicated the unnatural base pairs through multiple generations. This is the first known example of a living organism passing along an expanded genetic code to subsequent generations. This was in part achieved by the addition of a supportive algal gene that expresses a nucleotide triphosphate transporter which efficiently imports the triphosphates of both d5SICSTP and dNaMTP into E. coli bacteria. Then, the natural bacterial replication pathways use them to accurately replicate the plasmid containing d5SICS–dNaM.
The successful incorporation of a third base pair into a living micro-organism is a significant breakthrough toward the goal of greatly expanding the number of amino acids which can be encoded by DNA, thereby expanding the potential for living organisms to produce novel proteins. The artificial strings of DNA do not encode for anything yet, but scientists speculate they could be designed to manufacture new proteins which could have industrial or pharmaceutical uses.
In May 2014, researchers announced that they had successfully introduced two new artificial nucleotides into bacterial DNA, and by including individual artificial nucleotides in the culture media, were able to passage the bacteria 24 times; they did not create mRNA or proteins able to use the artificial nucleotides.
Selective pressure incorporation (SPI) method for production of alloproteins
There have been many studies that have produced protein with non-standard amino acids, but they do not alter the genetic code. These protein, called alloprotein, are made by incubating cells with an unnatural amino acid in the absence of a similar coded amino acid in order for the former to be incorporated into protein in place of the latter, for example L-2-aminohexanoic acid (Ahx) for methionine (Met).
These studies rely on the natural promiscuous activity of the aminoacyl tRNA synthetase to add to its target tRNA an unnatural amino acid (i.e. analog) similar to the natural substrate, for example methionyl-tRNA synthase's mistaking isoleucine for methionine. In protein crystallography, for example, the addition of selenomethionine to the media of a culture of a methionine-auxotrophic strain results in proteins containing selenomethionine as opposed to methionine (viz. Multi-wavelength anomalous dispersion for reason). Another example is that photoleucine and photomethionine are added instead of leucine and methionine to cross-label protein. Similarly, some tellurium-tolerant fungi can incorporate tellurocysteine and telluromethionine into their protein instead of cysteine and methionine. The objective of expanding the genetic code is more radical as it does not replace an amino acid, but it adds one or more to the code. On the other hand, proteome-wide replacements are most efficiently performed by global amino acid substitutions. For example, global proteome-wide substitutions of natural amino acids with fluorinated analogs have been attempted in E. coli and B. subtilis. A complete tryptophan substitution with thienopyrrole-alanine in response to 20899 UGG codons in E. coli was reported in 2015 by Budisa and Söll. Moreover, many biological phenomena, such as protein folding and stability, are based on synergistic effects at many positions in the protein sequence.
In this context, the SPI method generates recombinant protein variants or alloproteins directly by substitution of natural amino acids with unnatural counterparts. An amino acid auxotrophic expression host is supplemented with an amino acid analog during target protein expression. This approach avoids the pitfalls of suppression-based methods and it is superior to it in terms of efficiency, reproducibility and an extremely simple experimental setup. Numerous studies demonstrated how global substitution of canonical amino acids with various isosteric analogs caused minimal structural perturbations but dramatic changes in thermodynamic, folding, aggregation spectral properties and enzymatic activity.
in vitro synthesis
The genetic code expansion described above is in vivo. An alternative is the change of coding in vitro translation experiments. This requires the depletion of all tRNAs and the selective reintroduction of certain aminoacylated-tRNAs, some chemically aminoacylated.
There are several techniques to produce peptides chemically, generally it is by solid-phase protection chemistry. This means that any (protected) amino acid can be added into the nascent sequence.
In November 2017, a team from the Scripps Research Institute reported having constructed a semi-synthetic E. coli bacteria genome using six different nucleic acids (versus four found in nature). The two extra 'letters' form a third, unnatural base pair. The resulting organisms were able to thrive and synthesize proteins using "unnatural amino acids". The unnatural base pair used is dNaM–dTPT3. This unnatural base pair has been demonstrated previously, but this is the first report of transcription and translation of proteins using an unnatural base pair.
- Directed evolution
- List of genetic codes
- Nucleic acid analogue
- Protein labelling
- Protein methods
- Synthetic biology
- Xie, J; Schultz, PG (2005). "Adding amino acids to the genetic repertoire". Current Opinion in Chemical Biology. 9 (6): 548–54. doi:10.1016/j.cbpa.2005.10.011. PMID 16260173.
- Kubyshkin, V.; Acevedo-Rocha, C. G.; Budisa, N. (2017). "On universal coding events in protein biogenesis". Biosystems. 164: 16–25. doi:10.1016/j.biosystems.2017.10.004.
- Kubyshkin, V.; Budisa, N. (2017). "Synthetic alienation of microbial organisms by using genetic code engineering: Why and how?". Biotechnologie Journal. 12: 1600097. doi:10.1002/biot.201600097.
- Wang, L.; Brock, A.; Herberich, B.; Schultz, P. G. (April 2001). "Expanding the Genetic Code of Escherichia coli". Science. 292 (5516): 498–500. Bibcode:2001Sci...292..498W. doi:10.1126/science.1060077. PMID 11313494.
- Alberts, et. al, Bruce (2008). Molecular Biology of the Cell (5th ed.). New York: Garland Science. ISBN 0-8153-4105-9.
- Woese, et. al, Carl (2000). "Aminoacyl-tRNA synthetases, the genetic code, and the evolutionary process". Microbiol. Mol. Biol. Rev. 64: 202–236. doi:10.1128/mmbr.64.1.202-236.2000. PMC 98992. PMID 10704480.
- Sakamoto, K.; Hayashi, A.; Sakamoto, A.; Kiga, D.; Nakayama, H.; Soma, A.; Kobayashi, T.; Kitabatake, M.; et al. (2002). "Site-specific incorporation of an unnatural amino acid into proteins in mammalian cells". Nucleic Acids Res. 30 (21): 4692–4699. doi:10.1093/nar/gkf589. PMC 135798. PMID 12409460.
- Liu, C. C.; Schultz, P. G. (2010). "Adding new chemistries to the genetic code". Annual Review of Biochemistry. 79: 413–44. doi:10.1146/annurev.biochem.052308.105824. PMID 20307192.
- Summerer, D; Chen, S; Wu, N; Deiters, A; Chin, J. W.; Schultz, P. G. (2006). "A genetically encoded fluorescent amino acid". Proceedings of the National Academy of Sciences. 103 (26): 9785–9. Bibcode:2006PNAS..103.9785S. doi:10.1073/pnas.0603965103. PMC 1502531. PMID 16785423.
- Steinfeld, J. B.; Aerni, H. R.; Rogulina, S; Liu, Y; Rinehart, J (2014). "Expanded cellular amino acid pools containing phosphoserine, phosphothreonine, and phosphotyrosine". ACS Chemical Biology. 9 (5): 1104–12. doi:10.1021/cb5000532. PMC 4027946. PMID 24646179.
- Wang, L; Xie, J; Schultz, P. G. (2006). "Expanding the genetic code". Annual Review of Biophysics and Biomolecular Structure. 35: 225–49. doi:10.1146/annurev.biophys.35.101105.121507. PMID 16689635.
- Young, T. S.; Schultz, P. G. (2010). "Beyond the canonical 20 amino acids: Expanding the genetic lexicon". Journal of Biological Chemistry. 285 (15): 11039–44. doi:10.1074/jbc.R109.091306. PMC 2856976. PMID 20147747.
- Wang, L; Xie, J; Schultz, P. G. (2006). "Expanding the genetic code". Annual Review of Biophysics and Biomolecular Structure. 35: 225–49. doi:10.1146/annurev.biophys.35.101105.121507. PMID 16689635.
- "The Peter G. Schultz Laboratory". Schultz.scripps.edu. Retrieved 2015-05-05.
- Cardillo, G; Gentilucci, L; Tolomelli, A (March 2006). "Unusual amino acids: synthesis and introduction into naturally occurring peptides and biologically active analogues". Mini Reviews in Medicinal Chemistry. 6 (3): 293–304. doi:10.2174/138955706776073394. PMID 16515468.
- Journal of the American Chemical Society. 2003 Jan 29;125(4):935-9. Generation of a bacterium with a 21 amino acid genetic code. Mehl RA, Anderson JC, Santoro SW, Wang L, Martin AB, King DS, Horn DM, Schultz PG.
- "context :: 21-amino-acid bacteria: expanding the genetic code". Straddle3.net. Retrieved 2015-05-05.
- Koonin, E. V.; Novozhilov, A. S. (2009). "Origin and evolution of the genetic code: The universal enigma". IUBMB Life. 61 (2): 99–111. arXiv:0807.4749. doi:10.1002/iub.146. PMC 3293468. PMID 19117371.
- Taylor, Stanley R. Maloy, Valley J. Stewart, Ronald K. (1996). Genetic analysis of pathogenic bacteria : a laboratory manual. New York: Cold Spring Harbor Laboratory. ISBN 978-0-87969-453-1.
- Normanly, J; Kleina, L.G.; Masson, J.M.; Abelson, J.; Miller, J.H. (1990). "Construction of Escherichia coli amber suppressor tRNA genes. III. Determination of tRNA specificity". J. Mol. Biol. 213 (4): 719–726. doi:10.1016/S0022-2836(05)80258-X. PMID 2141650.
- Wang, L.; Magliery, T.J.; Liu, D.R.; Schultz, P.G. (2000). "A new functional suppressor tRNA/aminoacyl-tRNA synthetase pair for the in vivo incorporation of unnatural amino acids into proteins" (PDF). J. Am. Chem. Soc. 122 (20): 5010–5011. doi:10.1021/ja000595y.
- Wang, L; Brock, A; Herberich, B; Schultz, PG (20 April 2001). "Expanding the genetic code of Escherichia coli". Science. 292 (5516): 498–500. Bibcode:2001Sci...292..498W. doi:10.1126/science.1060077. PMID 11313494.
- Wang, L; Brock, A; Schultz, PG (6 March 2002). "Adding L-3-(2-Naphthyl)alanine to the genetic code of E. coli". Journal of the American Chemical Society. 124 (9): 1836–7. doi:10.1021/ja012307j. PMID 11866580.
- Chin, JW; Martin, AB; King, DS; Wang, L; Schultz, PG (20 August 2002). "Addition of a photocrosslinking amino acid to the genetic code of Escherichiacoli". Proceedings of the National Academy of Sciences of the United States of America. 99 (17): 11020–4. Bibcode:2002PNAS...9911020C. doi:10.1073/pnas.172226299. PMC 123203. PMID 12154230.
- Aerni, H. R.; Shifman, M. A.; Rogulina, S; O'Donoghue, P; Rinehart, J (2015). "Revealing the amino acid composition of proteins within an expanded genetic code". Nucleic Acids Research. 43 (2): e8. doi:10.1093/nar/gku1087. PMC 4333366. PMID 25378305.
- Isaacs, F. J.; Carr, P. A.; Wang, H. H.; Lajoie, M. J.; Sterling, B; Kraal, L; Tolonen, A. C.; Gianoulis, T. A.; Goodman, D. B.; Reppas, N. B.; Emig, C. J.; Bang, D; Hwang, S. J.; Jewett, M. C.; Jacobson, J. M.; Church, G. M. (2011). "Precise manipulation of chromosomes in vivo enables genome-wide codon replacement". Science. 333 (6040): 348–53. Bibcode:2011Sci...333..348I. doi:10.1126/science.1205822. PMID 21764749.
- Lajoie, M. J.; Rovner, A. J.; Goodman, D. B.; Aerni, H. R.; Haimovich, A. D.; Kuznetsov, G; Mercer, J. A.; Wang, H. H.; Carr, P. A.; Mosberg, J. A.; Rohland, N; Schultz, P. G.; Jacobson, J. M.; Rinehart, J; Church, G. M.; Isaacs, F. J. (2013). "Genomically recoded organisms expand biological functions". Science. 342 (6156): 357–60. Bibcode:2013Sci...342..357L. doi:10.1126/science.1241459. PMC 4924538. PMID 24136966.
- Mandell, D. J.; Lajoie, M. J.; Mee, M. T.; Takeuchi, R; Kuznetsov, G; Norville, J. E.; Gregg, C. J.; Stoddard, B. L.; Church, G. M. (2015). "Biocontainment of genetically modified organisms by synthetic protein design". Nature. 518 (7537): 55–60. Bibcode:2015Natur.518...55M. doi:10.1038/nature14121. PMC 4422498. PMID 25607366.
- Zeng, Y; Wang, W; Liu, W. R. (2014). "Towards reassigning the rare AGG codon in Escherichia coli". ChemBioChem. 15 (12): 1750–4. doi:10.1002/cbic.201400075. PMC 4167342. PMID 25044341.
- Bohlke, N; Budisa, N (February 2014). "Sense codon emancipation for proteome-wide incorporation of noncanonical amino acids: rare isoleucine codon AUA as a target for genetic code expansion". FEMS Microbiology Letters. 351 (2): 133–44. doi:10.1111/1574-6968.12371. PMC 4237120. PMID 24433543.
- Hoesl, M. G.; Budisa, N. (2012). "Recent advances in genetic code engineering in Escherichia coli". Current Opinion in Biotechnology. 23 (5): 751–7. doi:10.1016/j.copbio.2011.12.027. PMID 22237016.
- Neumann, H; Wang, K; Davis, L; Garcia-Alai, M; Chin, JW (18 March 2010). "Encoding multiple unnatural amino acids via evolution of a quadruplet-decoding ribosome". Nature. 464 (7287): 441–4. Bibcode:2010Natur.464..441N. doi:10.1038/nature08817. PMID 20154731.
- Watanabe, T; Muranaka, N; Hohsaka, T. (2008). "Four-base codon-mediated saturation mutagenesis in a cell-free translation system". J Biosci Bioeng. 105 (3): 211–5. doi:10.1263/jbb.105.211. PMID 18397770.
- Anderson, J.C.; Wu, N.; Santoro, S.W.; Lakshman, V.; King, D.S.; Schultz, P.G. (2004). "An expanded genetic code with a functional quadruplet codon". Proc Natl Acad Sci USA. 101 (20): 7566–7571. Bibcode:2004PNAS..101.7566A. doi:10.1073/pnas.0401517101. PMC 419646. PMID 15138302.
- Santoro, S.W.; Anderson, J.C.; Lakshman, V.; Schultz, P.G. (2003). "An archaebacteria-derived glutamyl-tRNA synthetase and tRNA pair for unnatural amino acid mutagenesis of proteins in Escherichia coli". Nucleic Acids Res. 31 (23): 6700–6709. doi:10.1093/nar/gkg903. PMC 290271. PMID 14627803.
- Anderson, J.C.; Schultz, P.G. (2003). "Adaptation of an orthogonal archaeal leucyl-tRNA and synthetase pair for four-base, amber, and opal suppression". Biochemistry. 42 (32): 9598–9608. doi:10.1021/bi034550w. PMID 12911301.
- Minaba, Masaomi; Kato, Yusuke (2013). "High-Yield, Zero-Leakage Expression System with a Translational Switch Using Site-Specific Unnatural Amino Acid Incorporation". Applied and Environmental Microbiology. 80 (5): 1718–1725. doi:10.1128/AEM.03417-13. PMC 3957627. PMID 24375139.
- Chin, J.W.; Cropp, T.A.; Anderson, J.C.; Mukherji, M.; Zhang, Z.; Schultz, P.G. (2003). "An expanded eukaryotic genetic code". Science. 301 (5635): 964–967. Bibcode:2003Sci...301..964C. doi:10.1126/science.1084772. PMID 12920298.
- Wu, N.; Deiters, A.; Cropp, T.A.; King, D.; Schultz, P.G. (2004). "A genetically encoded photocaged amino Acid". J Am Chem Soc. 126 (44): 14306–14307. doi:10.1021/ja040175z. PMID 15521721.
- Kowal, A.K.; Kohrer, C.; RajBhandary, U.L. (2001). "Twenty-first aminoacyl-tRNA synthetase–suppressor tRNA pairs for possible use in site-specific incorporation of amino acid analogues into proteins in eukaryotes and in eubacteria". Proc Natl Acad Sci USA. 98 (5): 2268–2273. Bibcode:2001PNAS...98.2268K. doi:10.1073/pnas.031488298. PMC 30127. PMID 11226228.
- Hancock, S. M.; Uprety, R; Deiters, A; Chin, J. W. (2010). "Expanding the genetic code of yeast for incorporation of diverse unnatural amino acids via a pyrrolysyl-tRNA synthetase/tRNA pair". Journal of the American Chemical Society. 132 (42): 14819–24. doi:10.1021/ja104609m. PMC 2956376. PMID 20925334.
- Kang, Ji-Yong (2013). "In vivo Expression of a Light-activatable Potassium Channel Using Unnatural Amino Acids". Neuron. 80: 358–370. doi:10.1016/j.neuron.2013.08.016. PMC 3815458. PMID 24139041.
- Zhang, Z.; Alfonta, L.; Tian, F.; Bursulaya, B.; Uryu, S.; King, D.S.; Schultz, P.G. (2004). "Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells". Proc. Natl. Acad. Sci. USA. 101 (24): 8882–8887. Bibcode:2004PNAS..101.8882Z. doi:10.1073/pnas.0307029101. PMC 428441. PMID 15187228.
- Wang, W.; Takimoto, J.; Louie, G.V.; Baiga, T.J.; Noel, J.P.; Lee, K.F.; Slesinger, P.A.; Wang, L. (2007). "Genetically encoding unnatural amino acids for cellular and neuronal studies". Nat. Neurosci. 10 (8): 1063–1072. doi:10.1038/nn1932. PMC 2692200. PMID 17603477.
- Han, S.; Yang, A.; Lee, S.; Lee, H.W.; Park, C.B.; Park, H.S. (2017). "Expanding the genetic code of Mus musculus". Nat. Commun. 8: 14568. Bibcode:2017NatCo...814568H. doi:10.1038/ncomms14568. PMC 5321798. PMID 28220771.
- Chin, J. W.; Rackham, Oliver (2005). "A network of orthogonal ribosomedotmRNA pairs". Nature Chemical Biology. 1 (3): 159–166. doi:10.1038/nchembio719.
- Wang, Kaihang; Neumann, Heinz; et al. (2007). "Evolved orthogonal ribosomes enhance the efficiency of synthetic genetic code expansion". Nature Biotechnology. 25 (7): 770–777. doi:10.1038/nbt1314.
- Fried, S. D.; Schmied, W. H.; Uttamapinant, C.; Chin, Jason W. (2015). "Ribosome Subunit Stapling for Orthogonal Translation in E. coli". Angewandte Chemie International Edition. 54 (43): 12791–12794. doi:10.1002/anie.201506311. PMC 4985662. PMID 27570300.
- Terasaka, Naohiro; Hayashi, Gosuke; Katoh, Takayuki; Suga, Hiroaki (2014). "An orthogonal ribosome-tRNA pair via engineering of the peptidyl transferase center". Nature Chemical Biology. 10 (7): 555–557. doi:10.1038/nchembio.1549.
- Cavarelli, J.; Moras, D. (1993). "Recognition of tRNAs by aminoacyl-tRNA synthetases". The FASEB Journal. 7 (1): 79–86. doi:10.1096/fasebj.7.1.8422978.
- Schimmel, Paul R.; Söll, Dieter (1979). "Aminoacyl-tRNA Synthetases: General Features and Recognition of Transfer RNAs". Annual Review of Biochemistry. 48: 601–648. doi:10.1146/annurev.bi.48.070179.003125. PMID 382994.
- Ohuchi, Masaki; Murakami, Hiroshi; Suga, Hiroaki (2007). "The flexizyme system: a highly flexible tRNA aminoacylation tool for the translation apparatus". Current Opinion in Chemical Biology. 11 (5): 537–542. doi:10.1016/j.cbpa.2007.08.011.
- Wang, Q; Parrish, AR; Wang, L (2009). "Expanding the Genetic Code for Biological Studies". Chemistry & Biology. 16 (3): 323–36. doi:10.1016/j.chembiol.2009.03.001. PMC 2696486. PMID 19318213.
- Park, Hee-Sung; Hohn, Michael J.; Umehara, Takuya; Guo, Li-Tao; Osborne, Edith M.; Benner, Jack; Noren, Christopher J.; Rinehart, Jesse; Söll, Dieter (2011-08-26). "Expanding the Genetic Code of Escherichia coli with Phosphoserine". Science. 333 (6046): 1151–1154. Bibcode:2011Sci...333.1151P. doi:10.1126/science.1207203. ISSN 0036-8075. PMID 21868676.
- Oza, Javin P.; Aerni, Hans R.; Pirman, Natasha L.; Barber, Karl W.; ter Haar, Charlotte M.; Rogulina, Svetlana; Amrofell, Matthew B.; Isaacs, Farren J.; Rinehart, Jesse (2015-09-09). "Robust production of recombinant phosphoproteins using cell-free protein synthesis". Nature Communications. 6: 8168. Bibcode:2015NatCo...6E8168O. doi:10.1038/ncomms9168. PMC 4566161. PMID 26350765.
- Pirman, Natasha L.; Barber, Karl W.; Aerni, Hans R.; Ma, Natalie J.; Haimovich, Adrian D.; Rogulina, Svetlana; Isaacs, Farren J.; Rinehart, Jesse (2015-09-09). "A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation". Nature Communications. 6: 8130. Bibcode:2015NatCo...6E8130P. doi:10.1038/ncomms9130. PMC 4566969. PMID 26350500.
- Rogerson, Daniel T; Sachdeva, Amit; Wang, Kaihang; Haq, Tamanna; Kazlauskaite, Agne; Hancock, Susan M; Huguenin-Dezot, Nicolas; Muqit, Miratul M K; Fry, Andrew M (2015-01-01). "Efficient genetic encoding of phosphoserine and its nonhydrolyzable analog". Nature Chemical Biology. 11 (7): 496–503. doi:10.1038/nchembio.1823. PMC 4830402. PMID 26030730.
- Gauba, V; Grünewald, J; Gorney, V; Deaton, L. M.; Kang, M; Bursulaya, B; Ou, W; Lerner, R. A.; Schmedt, C; Geierstanger, B. H.; Schultz, P. G.; Ramirez-Montagut, T (2011). "Loss of CD4 T-cell-dependent tolerance to proteins with modified amino acids". Proceedings of the National Academy of Sciences. 108 (31): 12821–6. Bibcode:2011PNAS..10812821G. doi:10.1073/pnas.1110042108. PMC 3150954. PMID 21768354.
- Liu, CC; Mack, AV; Brustad, EM; Mills, JH; Groff, D; Smider, VV; Schultz, PG. (2009). "The Evolution of Proteins with Genetically Encoded "Chemical Warheads"". J Am Chem Soc. 131 (28): 9616–7. doi:10.1021/ja902985e. PMC 2745334. PMID 19555063.
- Hammerling, M. J.; Ellefson, J. W.; Boutz, D. R.; Marcotte, E. M.; Ellington, A. D.; Barrick, J. E. (2014). "Bacteriophages use an expanded genetic code on evolutionary paths to higher fitness". Nature Chemical Biology. 10 (3): 178–80. doi:10.1038/nchembio.1450. PMC 3932624. PMID 24487692.
- Krishnakumar, R; Ling, J (31 January 2014). "Experimental challenges of sense codon reassignment: an innovative approach to genetic code expansion". FEBS Letters. 588 (3): 383–8. doi:10.1016/j.febslet.2013.11.039. PMID 24333334.
- Gibson, DG; Glass, JI; Lartigue, C; Noskov, VN; Chuang, RY; Algire, MA; Benders, GA; Montague, MG; Ma, L; Moodie, MM; Merryman, C; Vashee, S; Krishnakumar, R; Assad-Garcia, N; Andrews-Pfannkoch, C; Denisova, EA; Young, L; Qi, ZQ; Segall-Shapiro, TH; Calvey, CH; Parmar, PP; Hutchison CA, 3rd; Smith, HO; Venter, JC (2 July 2010). "Creation of a bacterial cell controlled by a chemically synthesized genome". Science. 329 (5987): 52–6. Bibcode:2010Sci...329...52G. doi:10.1126/science.1190719. PMID 20488990.
- Liang, X; Baek, CH; Katzen, F (20 December 2013). "Escherichia coli with two linear chromosomes". ACS Synthetic Biology. 2 (12): 734–40. doi:10.1021/sb400079u. PMID 24160891.
- Hirao, I.; et al. (2002). "An unnatural base pair for incorporating amino acid analogs into proteins". Nat. Biotechnol. 20: 177–182. doi:10.1038/nbt0202-177. PMID 11821864.
- Hirao, I.; et al. (2006). "An unnatural hydrophobic base pair system: site-specific incorporation of nucleotide analogs into DNA and RNA". Nat. Methods. 6: 729–735. doi:10.1038/nmeth915.
- Kimoto, M. et al. (2009) An unnatural base pair system for efficient PCR amplification and functionalization of DNA molecules. Nucleic acids Res. 37, e14
- Yamashige, R.; et al. (2011). "Highly specific unnatural base pair systems as a third base pair for PCR amplification". Nucleic Acids Res. 40: 2793–2806. doi:10.1093/nar/gkr1068. PMC 3315302. PMID 22121213.
- Kimoto, M.; et al. (2013). "Generation of high-affinity DNA aptamers using an expanded genetic alphabet". Nat. Biotechnol. 31: 453–457. doi:10.1038/nbt.2556. PMID 23563318.
- Malyshev, Denis A.; Dhami, Kirandeep; Quach, Henry T.; Lavergne, Thomas; Ordoukhanian, Phillip (24 July 2012). "Efficient and sequence-independent replication of DNA containing a third base pair establishes a functional six-letter genetic alphabet". Proceedings of the National Academy of Sciences of the United States of America. 109 (30): 12005–12010. Bibcode:2012PNAS..10912005M. doi:10.1073/pnas.1205176109. PMC 3409741. PMID 22773812. Retrieved 2014-05-11.
- Malyshev, Denis A.; Dhami, Kirandeep; Lavergne, Thomas; Chen, Tingjian; Dai, Nan; Foster, Jeremy M.; Corrêa, Ivan R.; Romesberg, Floyd E. (May 7, 2014). "A semi-synthetic organism with an expanded genetic alphabet". Nature. 509: 385–388. Bibcode:2014Natur.509..385M. doi:10.1038/nature13314. PMC 4058825. PMID 24805238. Retrieved May 7, 2014.
- Callaway, Ewan (May 7, 2014). "Scientists Create First Living Organism With 'Artificial' DNA". Nature News. Huffington Post. Retrieved 8 May 2014.
- Fikes, Bradley J. (May 8, 2014). "Life engineered with expanded genetic code". San Diego Union Tribune. Archived from the original on 9 May 2014. Retrieved 8 May 2014.
- Sample, Ian (May 7, 2014). "First life forms to pass on artificial DNA engineered by US scientists". The Guardian. Retrieved 8 May 2014.
- Pollack, Andrew (May 7, 2014). "Scientists Add Letters to DNA's Alphabet, Raising Hope and Fear". The New York Times. Retrieved 8 May 2014.
- Pollack, Andrew (May 7, 2014). "Researchers Report Breakthrough in Creating Artificial Genetic Code". The New York Times. Retrieved May 7, 2014.
- Callaway, Ewen (May 7, 2014). "First life with 'alien' DNA". Nature. doi:10.1038/nature.2014.15179. Retrieved May 7, 2014.
- Amos, Jonathan (8 May 2014). "Semi-synthetic bug extends 'life's alphabet'". BBC News. Retrieved 2014-05-09.
- Koide, H.; Yokoyama, S.; Kawai, G.; Ha, J. M.; Oka, T.; Kawai, S.; Miyake, T.; Fuwa, T.; Miyazawa, T. (1988). "Biosynthesis of a protein containing a nonprotein amino acid by Escherichia coli: L-2-aminohexanoic acid at position 21 in human epidermal growth factor". Proceedings of the National Academy of Sciences of the United States of America. 85 (17): 6237–6241. Bibcode:1988PNAS...85.6237K. doi:10.1073/pnas.85.17.6237. PMC 281944. PMID 3045813.
- Ferla, M. P.; Patrick, W. M. (2014). "Bacterial methionine biosynthesis". Microbiology. 160 (Pt 8): 1571–84. doi:10.1099/mic.0.077826-0. PMID 24939187.
- Doublié, S. (2007). "Production of Selenomethionyl Proteins in Prokaryotic and Eukaryotic Expression Systems". Macromolecular Crystallography Protocols. Methods in Molecular Biology. 363. p. 91. doi:10.1007/978-1-59745-209-0_5. ISBN 978-1-58829-292-6.
- Suchanek, Monika; Radzikowska, Anna; Thiele, Christoph (2005). "Photo-leucine and photo-methionine allow identification of protein-protein interactions in living cells". Nature Methods. 2 (4): 261–268. doi:10.1038/NMETH752. PMID 15782218.
- Ramadan, S. E.; Razak, A. A.; Ragab, A. M.; El-Meleigy, M. (1989). "Incorporation of tellurium into amino acids and proteins in a tellurium-tolerant fungi". Biological trace element research. 20 (3): 225–232. doi:10.1007/BF02917437. PMID 2484755.
- Bacher, J. M.; Ellington, A. D. (2001). "Selection and characterization of Escherichia coli variants capable of growth on an otherwise toxic tryptophan analogue". Journal of Bacteriology. 183: 5414–5425. doi:10.1128/jb.183.18.5414-5425.2001. PMC 95426. PMID 11514527.
- Wong, J. T. (1983). "Membership mutation of the genetic code: Loss of fitness by tryptophan". Proc. Natl. Acad. Sci. USA. 80: 6303–6306. Bibcode:1983PNAS...80.6303W. doi:10.1073/pnas.80.20.6303. PMC 394285. PMID 6413975.
- Hoesl, M. G.; Oehm, S.; Durkin, P.; Darmon, E.; Peil, L.; Aerni, H.-R.; Rappsilber, J.; Rinehart, J.; Leach, D.; Söll, D.; Budisa, N. (2015). "Chemical evolution of a bacterial proteome". Angewandte Chemie International Edition. 54: 10030–10034. doi:10.1002/anie.201502868. PMC 4782924. PMID 26136259. NIHMSID: NIHMS711205
- Moroder, L.; Budisa, N. (2010). "Synthetic biology of protein folding". ChemBioChem. 11: 1181–7. doi:10.1002/cphc.201000035. PMID 20391526.
- Budisa, N. (2004). "Prolegomena to future efforts on genetic code engineering by expanding its amino acid repertoire". Angewandte Chemie International Edition. 43: 3387–3428. doi:10.1002/anie.20030064.
- Link, A. J.; Mock, M. L.; Tirell, D. A. (2003). "Non-canonical amino acids in protein engineering". Curr Opin Biotechnol. 14: 603–9. doi:10.1016/j.copbio.2003.10.011. PMID 14662389.
- Nehring, S.; Budisa, N.; Wiltschi, B. (2012). "Performance analysis of orthogonal pairs designed for an expanded eukaryotic genetic code". PLOS ONE. 7 (4): e31992. Bibcode:2012PLoSO...731992N. doi:10.1371/journal.pone.0031992. PMC 3320878. PMID 22493661.
- Agostini, F.; Völler, J-S.; Koksch, B.; Acevedo-Rocha, C. G.; Kubyshkin, V.; Budisa, N. (2017). "Biocatalysis with Unnatural Amino Acids: Enzymology Meets Xenobiology". Angewandte Chemie International Edition. 56: 9680–9703. doi:10.1002/anie.201610129. PMID 28085996.
- Rubini, M.; Lepthien, S.; Golbik, R.; Budisa, N. (2006). "Aminotryptophan-containing barstar: structure--function tradeoff in protein design and engineering with an expanded genetic code". Biochim Biophys Acta. 1764 (7): 1147–58. doi:10.1016/j.bbapap.2006.04.012. PMID 16782415.
- Steiner, T.; Hess, P.; Bae, J. H.; Moroder, L.; Budisa, N. (2008). "Synthetic Biology of Proteins: Tuning GFP´s Folding and Stability with Fluoroproline". PLOS ONE. 3: e1680. Bibcode:2008PLoSO...3.1680S. doi:10.1371/journal.pone.0001680. PMC 2243022. PMID 18301757.
- Wolschner, C.; Giese, A.; Kretzschmar, H.; Huber, R.; Moroder, L.; Budisa, N. (2009). "Design of anti- and pro-aggregation variants to assess the effects of methionine oxidation in human prion protein" (PDF). Proc. Natl. Acad. Sci. USA. 106: 7756–7761. Bibcode:2009PNAS..106.7756W. doi:10.1073/pnas.0902688106. PMC 2674404. PMID 19416900.
- Lepthien, S.; Hoesl, M. G.; Merkel, L.; Budisa, N. (2008). "Azatryptophans endow proteins with intrinsic blue fluorescence". Proc. Natl. Acad. Sci. USA. 105 (42): 16095–16100. Bibcode:2008PNAS..10516095L. doi:10.1073/pnas.0802804105. PMC 2571030. PMID 18854410.
- Bae, J.; Rubini, M.; Jung, G.; Wiegand, G.; Seifert, M. H. J.; Azim, M. K.; Kim, J. S.; Zumbusch, A.; Holak, T. A.; Moroder, L.; Huber, R.; Budisa, N. (2003). "Expansion of the Genetic Code Enables Design of a Novel "Gold" Class of Green Fluorescent Proteins". Journal of Molecular Biology. 328: 977–1202. doi:10.1016/s0022-2836(03)00364-4. PMID 12729742.
- Hoesl, M. G.; Acevedo-Rocha, C. G.; Nehring, S.; Royter, M.; Wolschner, C.; Wiltschi, B.; Budisa, N.; Antranikian, G. (2011). "Lipase Congeners Designed by Genetic Code Engineering". ChemCatChem. 3 (1): 213–221. doi:10.1002/cctc.201000253. ISSN 1867-3880.
- Hong, SH; Kwon, YC; Jewett, MC (2014). "Non-standard amino acid incorporation into proteins using Escherichia coli cell-free protein synthesis". Frontiers in Chemistry. 2: 34. Bibcode:2014FrCh....2...34H. doi:10.3389/fchem.2014.00034. PMC 4050362. PMID 24959531.
- 'Unnatural' microbe can make proteins. BBC News. 29 November 2017.
- A semi-synthetic organism that stores and retrieves increased genetic information. Yorke Zhang, Jerod L. Ptacin, Emil C. Fischer, Hans R. Aerni, Carolina E. Caffaro, Kristine San Jose, Aaron W. Feldman, Court R. Turner, and Floyd E. Romesberg. Nature vol 551, pp: 644–647. 30 November 2017. doi:10.1038/nature24659
- On stranger nucleotides. Josh Howgego, Chemistry World. 25 February 2014.
- Li, L; Degardin, M; Lavergne, T; Malyshev, DA; Dhami, K; Ordoukhanian, P; Romesberg, FE (2014). "Natural-like replication of an unnatural base pair for the expansion of the genetic alphabet and biotechnology applications". J Am Chem Soc. 136: 826–9. doi:10.1021/ja408814g. PMC 3979842. PMID 24152106.