Thalidomide

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This article is about the drug. For the musical about a person with Thalidomide disability, see Thalidomide!! A Musical.
Thalidomide
Clinical data
Pregnancy
category
  • AU: X (High risk)
Routes of
administration		oral
ATC code
Legal status
Legal status
  • In general: ℞ (Prescription only)
Pharmacokinetic data
Protein binding55% and 66% for the (+)-R and (–)-S enantiomers, respectively
MetabolismHepatic (CYP2C19)[1]
Elimination half-lifemean ranges from approximately 5 to 7 hours following a single dose; not altered with multiple doses
Identifiers
  • 2-(2,6-dioxopiperidin-3-yl)-1H-isoindole-1,3(2H)-dione
CAS Number
PubChem CID
DrugBank
ChemSpider
CompTox Dashboard (EPA)
ECHA InfoCard100.000.029 Edit this at Wikidata
Chemical and physical data
FormulaC13H10N2O4
Molar mass258.23 g/mol g·mol−1
3D model (JSmol)
  • O=C1NC(=O)CCC1N3C(=O)c2ccccc2C3=O
  (verify)

Thalidomide (Template:Pron-en) is a sedative-hypnotic and multiple myeloma medication. The drug is a potent teratogen in rabbits and primates including humans: severe birth defects may result if the drug is taken during pregnancy.[2]

Thalidomide was sold in a number of countries across the world from 1957 until 1961 when it was withdrawn from the market after being found to be a cause of birth defects in what has been called "the biggest medical tragedy of modern times".[3] It is not known exactly how many worldwide victims of the drug there have been, although estimates range from 10,000 to 20,000.[4]

Since then thalidomide has been found to be a valuable treatment for a number of medical conditions and it is being prescribed again in a number of countries, although its use remains controversial.[5][6] The thalidomide tragedy led to much stricter testing being required for drugs and pesticides before they can be licensed.[7]

History

Development

Thalidomide was said to have been developed by German pharmaceutical company Grünenthal in Stolberg (Rhineland) near Aachen, although this claim has recently been challenged. A report published by Dr Martin W Johnson, director of the Thalidomide Trust in the United Kingdom, detailed evidence that suggested the drug had been developed under the direction of a Nazi scientist in 1944, as an antidote to nerve gases such as sarin, ten years before Grünenthal secured a patent in 1954.[8] Other sources have suggested that it may have been first synthesised by British scientists at the University of Nottingham in 1949.[9] Thalidomide was found to act as an effective tranquiliser and painkiller and was proclaimed as a "wonder drug" for insomnia, coughs, colds and headaches. It was also found to be an effective antiemetic which had an inhibitory effect on morning sickness, and so thousands of pregnant women took the drug to relieve their symptoms.[4] At the time of the drug's development it was not thought likely that any drug could pass from the mother across the placental barrier and harm the developing fetus.[7]

Birth defects

File:Thalidomide Baby.jpg
1962 photo of a baby born to a mother who had taken Thalidomide while pregnant; note the extra appendage connected to the foot.

In the late 1950s and early 1960s, more than 10,000 children in 46 countries were born with deformities such as phocomelia, as a consequence of thalidomide use.[10] The Australian obstetrician William McBride and the German pediatrician Widukind Lenz suspected a link between birth defects and the drug, and this was proved by Lenz in 1961.[11][12] McBride was later awarded a number of honours including a medal and prize money by the prestigious L'Institut de la Vie in Paris.[13]

In the United Kingdom the drug was licensed in 1958 and, of the babies born with defects, 456 survived. The drug was withdrawn in 1961 but it was not until 1968, after a long campaign by The Sunday Times newspaper, that a compensation settlement for the UK victims was reached with Distillers Company Limited.[14][15] In Germany approximately 2,500 thalidomide babies were born.[12]

1962: FDA inspector Frances Oldham Kelsey receives an award from President John F. Kennedy for blocking sale of Thalidomide in the United States

The impact in the United States was minimized when pharmacologist and M.D. Frances Oldham Kelsey refused Food and Drug Administration (FDA) approval for an application from Richardson Merrell to market thalidomide, saying more study was needed. Richardson Merrell gave the tablets to doctors on the understanding that the drug was still under investigation. Seventeen children were born in the U.S. with the defects.[10]

In 1962, the United States Congress enacted laws requiring tests for safety during pregnancy before a drug can receive approval for sale in the U.S.[16] Other countries enacted similar legislation, and thalidomide was not prescribed or sold for decades.

Mechanism

It was soon discovered that only one particular optical isomer of thalidomide caused the teratogenicity. The pair of enantiomers, while mirror images of each other, cause different effects[17], although it is now known that the "safe" isomer can be converted to the teratogenic one in the human body.[12][18](see Teratogenic mechanism).

Revived interest

In 1964 Jacob Sheskin, Professor at the Hebrew University of Jerusalem at Hadassah University Hospital (he was also the chief staff and manager of Hansen Leper Hospital in Jerusalem), administered thalidomide to a critically ill patient with erythema nodosum leprosum (ENL), a painful complication of leprosy, in an attempt to relieve his pain in spite of the ban. The patient slept for hours, and was able to get out of bed without aid upon awakening. The result was followed by more favorable experiences and then by a clinical trial.[19]

He found that patients with erythema nodosum leprosum, a painful skin condition, experienced relief of their pain by taking thalidomide. Further work conducted in 1991 by Dr. Gilla Kaplan at Rockefeller University in New York City showed that thalidomide worked in leprosy by inhibiting tumor necrosis factor alpha. Kaplan partnered with Celgene Corporation to further develop the potential for thalidomide. Subsequent research has shown that thalidomide is effective in the treatment of multiple myeloma, and it was approved for use in the United States by the FDA for use in this malignancy. The FDA has also since approved the drug's use in the treatment of erythema nodosum leprosum. There are studies underway to determine the drug's effects on arachnoiditis and several types of cancers. However, physicians and patients alike must go through a special process to prescribe and receive thalidomide (S.T.E.P.S) to ensure no more children are born with birth defects traceable to the medication. Celgene Corporation has also developed analogues to thalidomide, such as lenalidomide, that are substantially more powerful and have fewer side effects - except for greater myelosuppression.[20]

United States

On July 16, 1998, the FDA approved the use of thalidomide for the treatment of lesions associated with Erythema Nodosum Leprosum (ENL). Because of thalidomide’s potential for causing birth defects, the distribution of the drug was permitted only under tightly controlled conditions. The FDA required that Celgene Corporation, which planned to market thalidomide under the brand name Thalomid, establish a System for Thalidomide Education and Prescribing Safety (S.T.E.P.S) oversight program. The conditions required under the program include; limiting prescription and dispensing rights only to authorized prescribers and pharmacies, keeping a registry of all patients prescribed thalidomide, providing extensive patient education about the risks associated with the drug and providing periodic pregnancy tests for women who are prescribed it.[21] On May 26, 2006, the U.S. Food and Drug Administration granted accelerated approval for thalidomide (Thalomid, Celgene Corporation) in combination with dexamethasone for the treatment of newly diagnosed multiple myeloma (MM) patients.[22] The FDA approval came seven years after the first reports of efficacy in the medical literature[23] and Celgene took advantage of "off-label" marketing opportunities to promote the drug in advance of its FDA approval for the myeloma indication. Thalomid, as the drug is commercially known, sold over $300 million per year, while only approved for leprosy.[24]

United Kingdom

Thalidomide is available to only a small number of patients in the UK, generally in specialist cancer treatment centres where research trials are taking place and specialist doctors have experience in its use.[25]

Brazil

Brazil has the highest prevalence rate of leprosy in the world and thalidomide has been used by Brazilian physicians as the drug of choice for the treatment of severe ENL since 1965. A study published in 1994 found 61 people born after 1965 whose limb defects and exposure history were compatible with thalidomide embryopathy. In 63.6% of these cases, thalidomide had been prescribed without the physician informing the patient about the drug's teratogenicity. Since then production, dispensing and prescription of thalidomide have been strictly controlled and no cases of thalidomide embryopathy are thought to have occurred since 1997.[26]

Possible indications

Research on thalidomide slowed in the 1960s, but never stopped. At least one university in the United States pursues thalidomide research, even though performed by only one tenured professor. The medication is an example of how potentially dangerous compounds can be used therapeutically with appropriate precautions and procedures.

Serious infections including sepsis and tuberculosis cause the level of Tumor necrosis factor-alpha (TNFα) to rise. TNFα is a chemical mediator in the body, and it may enhance the wasting process in cancer patients as well. Thalidomide may reduce the levels of TNFα, and it is possible that the drug's effect on ENL is caused by this mechanism.[16]

Thalidomide also has potent anti-inflammatory effects that may help ENL patients. In July 1998, the FDA approved the application of Celgene to distribute thalidomide under the brand name Thalomid for treatment of ENL. Pharmion Corporation, who licensed the rights to market Thalidomide in Europe, Australia and various other territories from Celgene, received approval for its use against multiple myeloma in Australia and New Zealand in 2003.[27] Thalomid, in conjunction with dexamethasone, is now standard therapy for multiple myeloma.

Thalidomide is also prescribed for its anti-inflammatory effects in actinic prurigo, an autoimmune skin disease.

Thalidomide also inhibits the growth of new blood vessels (angiogenesis), which may be useful in treating macular degeneration and other diseases. This effect helps AIDS patients with Kaposi's sarcoma, although there are better and cheaper drugs to treat the condition. Thalidomide may be able to fight painful, debilitating aphthous lesions in the mouth and esophagus of AIDS patients which prevent them from eating. The FDA formed a Thalidomide Working Group in 1994 to provide consistency between its divisions, with particular emphasis on safety monitoring. The agency also imposed severe restrictions on the distribution of Thalomid through the System for Thalidomide Education and Prescribing Safety (STEPS) program.[16]

Thalidomide is also being investigated for treating symptoms of prostate cancer, glioblastoma, lymphoma, arachnoiditis, Behçet's disease, and Crohn's disease. In a small trial, Australian researchers found thalidomide sparked a doubling of the number of T cells in patients, allowing the patients' own immune system to attack cancer cells.

Full list of indications currently being investigated in clinical trials;[28]

  • Amyotrophic lateral sclerosis
  • Aphthous ulcer
  • Behcet's syndrome
  • Brain cancer
  • Breast cancer
  • Cachexia
  • Colorectal cancer
  • Congestive heart failure
  • Crohn's disease
  • Diarrhoea
  • Fibrodysplasia ossificans progressiva
  • Graft-versus-host disease
  • Haematological malignancies
  • HIV infections
  • Hodgkin's disease
  • Kaposi's sarcoma
  • Leukaemia
  • Macular degeneration
  • Malignant melanoma
  • Mycobacterium avium complex infections
  • Myelodysplastic syndromes
  • Myelofibrosis
  • Myeloid leukaemia
  • Non-Hodgkin's lymphoma
  • Non-small cell lung cancer
  • Ovarian cancer
  • Pain
  • Prostate cancer
  • Prurigo nodularis
  • Renal cancer
  • Rheumatoid arthritis
  • Small cell lung cancer
  • Systemic lupus erythematosus
  • Thyroid cancer
  • Tuberculosis

Thalidomide and multiple myeloma

Thalidomide was first tested as a single agent for the treatment of multiple myeloma in 1999 for its antiangiogenesis activity.[29] Since then many studies have shown that thalidomide in combination with dexamethasone has increased the survival of multiple myeloma patients. The combination of thalidomide and dexamethasone, often in combination with melphalan, is now one of the most common regimens for patients with newly diagnosed multiple myeloma, with an improved response rate of up to 60-70%.[30][31] However, thalidomide may also cause side effects such as polyneuropathy, fatigue, skin rash, and venous thromboembolism (VTE), or blood clots, which could lead to stroke or myocardial infarction.[32] Bennett et al. have conducted a systematic review of VTE associated with thalidomide in multiple myeloma patients.[33] They have found that when Thalidomide was administered without prophylaxis, VTE rates reached as high as 26%. Owing to the high rates of VTE associated with thalidomide in combination with dexamethasone or doxorubicin, a black box warning was added in 2006 to the package insert for thalidomide, indicating that patients with multiple myeloma who receive thalidomide-dexamethasone may benefit from concurrent thromboembolism prophylaxis or aspirin. In addition, owing to these side effects, newer drugs, such as a thalidomide derivative lenalidomide (marketed as Revlimid) and bortezomib (marketed as Velcade) have increased in popularity.[citation needed]

Teratogenic mechanism

The two enantiomers of thalidomide:
Left: (S)-thalidomide
Right: (R)-thalidomide

Thalidomide is racemic – it contains both left- and right-handed isomers in equal amounts. The (R) enantiomer is effective against morning sickness but the (S) is teratogenic and causes birth defects. The enantiomers can interconvert in vivo[34] – that is, if a human is given pure (R)-thalidomide or (S)-thalidomide, both isomers can be found in the serum – therefore, administering only one enantiomer will not prevent the teratogenic effect.

The mechanism of thalidomide's teratogenic action has led to over 2000 research papers and the proposal of fifteen or sixteen plausible mechanisms.[35] A theoretical synthesis in 2000[35] suggested the following mechanism: Thalidomide intercalates (inserts itself) into DNA in G-C (guanine-cytosine) rich regions.[36][37] Owing to its glutarimide part, (S) thalidomide fits neatly into the major groove of DNA at purine sites.[35] Such intercalation impacts upon the promoter regions of the genes controlling the development of limbs, ears, and eyes such as IGF-I and FGF-2. These normally activate the production of the cell surface attachment integrin αvβ3 with the resulting alphavbeta3 integrin dimer stimulating angiogenesis in developing limb buds. This then promotes the outgrowth of the bud (IGF-I and FGF-2 are also both known to stimulate angiogenesis). Therefore, by inhibiting the chain of events, thalidomide causes the truncation of limb development. In 2009 this theory[35] received strong support, with research showing "conclusively that loss of newly formed blood vessels is the primary cause of thalidomide teratogenesis, and developing limbs are particularly susceptible because of their relatively immature, highly angiogenic vessel network."[38]

Other side effects

Apart from its infamous tendency to induce birth defects and peripheral neuropathy, the main side effects of thalidomide include fatigue and constipation.[citation needed] It is also associated with an increased risk of deep vein thrombosis especially when combined with dexamethasone, as it is for treatment of multiple myeloma.[citation needed] High doses can lead to pulmonary oedema, atelectasis, aspiration pneumonia and refractory hypotension.[citation needed] In multiple myeloma patients, concomitant use with zoledronic acid may lead to increased incidence of renal dysfunction.[citation needed]

Thalidomide analogs

The exploration of the antiangiogenic and immunomodulatory activities of thalidomide has led to the study and creation of thalidomide analogs. In 2005, Celgene received FDA approval for lenalidomide (Revlimid) as the first commercially useful derivative. Revlimid is only available in a restricted distribution setting to avoid its use during pregnancy. Further studies are conducted to find safer compounds with useful qualities. Another analog, Actimid (CC-4047), is in the clinical trial phase.[39] These thalidomide analogs can be used to treat different diseases, or used in a regimen to fight two conditions.

Notable children affected

  • Mat Fraser, a comedian, actor, co-presenter of the BBC's Ouch Podcast.
  • Alison Lapper is an English artist who was the subject of Marc Quinn's sculpture Alison Lapper Pregnant, which was on display in Trafalgar Square in London from 2005 until late 2007.
  • Alvin Law, a motivational speaker and former radio broadcaster.
  • Tony Meléndez is a guitarist who was born without arms. He plays only with his feet.
  • Terry Wiles internationally known through On Giants' Shoulders, a book and film about his life.
  • Brett Nielsen, a musician , was the first Australian thalidomide child.
  • Louise Medus Mansell, daughter of David Mason, campaigner for increased compensation for Thalidomide children, born with no arms or legs.[40]
  • Thomas Quasthoff is an internationally acclaimed bass-baritone who describes himself: "1.34 meters tall, short arms, seven fingers - four right, three left - large, relatively well formed head, brown eyes, distinctive lips; profession: singer."[41]
  • Matthias Berg is a lawyer, renowned hornist and multiple Paralympics gold medalist.
  • Melody Potter Native Alabamian disability activist, severe deformities, no arms or legs.
  • Giancarlo Cosio, Italian Waterski World disabled Champion.
  • David Lega, Swedish swimmer. Paralympian and current holder of 5 world records.[42]
  • Gary Skyner, a comedian and motivational speaker.
  • Frankie Shum, an actor, starred in Kung fu films Crippled Masters and Crippled Heroes.

References

  1. ^ Ando Y, Fuse E, Figg WD (2002). "Thalidomide metabolism by the CYP2C subfamily". Clinical Cancer Research : an Official Journal of the American Association for Cancer Research. 8 (6): 1964–73. PMID 12060642. Retrieved 2009-06-18. {{cite journal}}: Unknown parameter |day= ignored (help); Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  2. ^ Thalidomide: Drug safety during pregnancy and breastfeeding / DRUGSAFETYSITE.COM
  3. ^ Anon. "Thalidomide - A Second Chance? - programme summary". BBC. Retrieved 2009-05-01.
  4. ^ a b Anon. "Born Freak". Happy Birthday Thalidomide. Channel 4. Retrieved 2009-05-01.
  5. ^ Anon (March 10, 2006). "Thalidomide:controversial treatment for multiple myeloma". Health news. Retrieved 2009-05-01.
  6. ^ Bowditch, Gillian (March 26, 2006). "Can thalidomide ever be trusted?". The Sunday Times. News International Limited. Retrieved 2009-05-01.
  7. ^ a b Heaton, C. A. (1994). The Chemical Industry. Springer. p. 40. ISBN 0751400181.
  8. ^ Foggo, Daniel (2009-02-08). "Thalidomide 'was created by the Nazis'". London: The Times. Retrieved 2009-06-18.
  9. ^ Foggo, Daniel (13 September 2009). "Thalidomide victim Gary Syner to go on hunger strike". The Sunday Times. Times Newspapers Ltd. Retrieved 2009-09-28.
  10. ^ a b Bren, Linda (2001-02-28). "Frances Oldham Kelsey: FDA Medical Reviewer Leaves Her Mark on History". FDA Consumer. US Food and Drug Administration. Retrieved 2006-09-21. {{cite news}}: Cite has empty unknown parameter: |coauthors= (help)
  11. ^ Anon. "Widukind Lenz". who name it?. Ole Daniel Enersen. Retrieved 2009-05-01.
  12. ^ a b c Anon. "Thalidomide:40 years on". BBC news. BBC. Retrieved 2009-05-01.
  13. ^ Report of Thalidomide at University of New South Wales. See also main William McBride article.
  14. ^ Ryan, Caroline (1st April 2004). "They just didn't know what it would do". BBC News:Health. BBC news. Retrieved 2009-05-01. {{cite web}}: Check date values in: |date= (help)
  15. ^ Flintoff, John-Paul (March 23 2008). "Thalidomide: the battle for compensation goes on". The Sunday Times. Times Newspapers Ltd. Retrieved 2009-05-01. {{cite news}}: Check date values in: |date= (help)
  16. ^ a b c Burkholz, Herbert (1997-09-01). "Giving Thalidomide a Second Chance". FDA Consumer. US Food and Drug Administration. Retrieved 2006-09-21.
  17. ^ Eccles H; Ratcliff B (2001). Chemistry 2. Cambridge University Press. p. 170. ISBN 978-0-521-79882-2.{{cite book}}: CS1 maint: multiple names: authors list (link)
  18. ^ Ligham, Alex (April 2000). "Optical Isomerism In Thalidomide". Thalidomide. Retrieved 2009-05-02.
  19. ^ Silverman, MD, William (2002-04-22). "The Schizophrenic Career of a "Monster Drug"". Pediatrics. 110 (2): 404–406. doi:10.1542/peds.110.2.404. PMID 12165600. Retrieved 2006-09-21. {{cite journal}}: Cite has empty unknown parameter: |coauthors= (help)
  20. ^ Rao KV (2007). "Lenalidomide in the treatment of multiple myeloma". American Journal of Health-system Pharmacy : AJHP : Official Journal of the American Society of Health-System Pharmacists. 64 (17): 1799–807. doi:10.2146/ajhp070029. PMID 17724360. {{cite journal}}: |access-date= requires |url= (help); Unknown parameter |month= ignored (help)
  21. ^ FDA, Center for Drug Evaluation and Research, July 16, 1998
  22. ^ FDA Approves Thalomid (thalidomide) to Treat Multiple Myeloma
  23. ^ Desikan, R (1999). "Activity of thalidomide (THAL) in multiple myeloma (MM) confirmed in 180 patients with advanced disease". Blood. 94 (Suppl. 1): 603a–603a. {{cite journal}}: Unknown parameter |coauthors= ignored (|author= suggested) (help)
  24. ^ Ismail, MA (2005-07-07). "FDA: A Shell of its Former Self". Pushing Prescriptions. The Centre for Public Integrity.
  25. ^ Anon. "Thalidomide". Cancer treatments. Cancerbackup. Retrieved 2009-05-01.
  26. ^ Paumgartten, J.R.; Chahoud, I (July 2006). "Thalidomide embryopathy cases in Brazil after 1965". Reproductive Toxicology. 22 (1). Elselvier: 1, 2. doi:10.1016/j.reprotox.2005.11.007. PMID 16427249. {{cite journal}}: More than one of |first1= and |first= specified (help); More than one of |last1= and |last= specified (help); Unknown parameter |coauthors= ignored (|author= suggested) (help)
  27. ^ Rouhi, Maureen. "Thalidomide". Chemical & Engineering News. American Chemical Society. Retrieved 2006-09-21.
  28. ^ . ADIS R&D Insight http://bi.adisinsight.com/rdi/viewdocument.aspx?render=view&mode=remote&adnm=800004827&PushValidation=121745. Retrieved 2009-08-21. {{cite news}}: Missing or empty |title= (help)
  29. ^ Singhal S, Mehta J, Desikan R; et al. (1999). "Antitumor activity of thalidomide in refractory multiple myeloma". The New England Journal of Medicine. 341 (21): 1565–71. doi:10.1056/NEJM199911183412102. PMID 10564685. Retrieved 2009-06-18. {{cite journal}}: Explicit use of et al. in: |author= (help); Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  30. ^ Gieseler F (2008). "Pathophysiological considerations to thrombophilia in the treatment of multiple myeloma with thalidomide and derivates". Thrombosis and Haemostasis. 99 (6): 1001–7. doi:10.1160/TH08-01-0009. PMID 18521500. {{cite journal}}: |access-date= requires |url= (help); Unknown parameter |month= ignored (help)
  31. ^ Denz U, Haas PS, Wäsch R, Einsele H, Engelhardt M (2006). "State of the art therapy in multiple myeloma and future perspectives". European Journal of Cancer (Oxford, England : 1990). 42 (11): 1591–600. doi:10.1016/j.ejca.2005.11.040. PMID 16815703. {{cite journal}}: |access-date= requires |url= (help); Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  32. ^ Haas PS, Denz U, Ihorst G, Engelhardt M (2008). "Thalidomide in consecutive multiple myeloma patients: single-center analysis on practical aspects, efficacy, side effects and prognostic factors with lower thalidomide doses". Eur. J. Haematol. 80 (4): 303–9. doi:10.1111/j.1600-0609.2007.01022.x. PMID 18182082. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  33. ^ Bennett CL, Angelotta C, Yarnold PR; et al. (2006). "Thalidomide- and lenalidomide-associated thromboembolism among patients with cancer". JAMA : the Journal of the American Medical Association. 296 (21): 2558–60. doi:10.1001/jama.296.21.2558-c. PMID 17148721. {{cite journal}}: |access-date= requires |url= (help); Explicit use of et al. in: |author= (help); Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  34. ^ Teo SK, Colburn WA, Tracewell WG, Kook KA, Stirling DI, Jaworsky MS, Scheffler MA, Thomas SD, Laskin OL (2004). "Clinical pharmacokinetics of thalidomide". Clin Pharmacokinet. 43 (5): 311–327. doi:10.2165/00003088-200443050-00004. PMID 15080764.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  35. ^ a b c d Stephens TD, Bunde CJ, Fillmore BJ (2000). "Mechanism of action in thalidomide teratogenesis". Biochemical Pharmacology. 59 (12): 1489–99. doi:10.1016/S0006-2952(99)00388-3. PMID 10799645. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  36. ^ Koch HP, Czejka MJ. (1986). Evidence for the intercalation of thalidomide into DNA: clue to the molecular mechanism of thalidomide teratogenicity? Z Naturforsch [C]. 41(11-12):1057-61. PMID 2953123
  37. ^ Huang PH, McBride WG (1997). "Interaction of [glutarimide-2-14C]-thalidomide with rat embryonic DNA in vivo". Teratogenesis, Carcinogenesis, and Mutagenesis. 17 (1): 1–5. doi:10.1002/(SICI)1520-6866(1997)17:1<1::AID-TCM2>3.0.CO;2-L. PMID 9249925.
  38. ^ Therapontos C, Erskine L, Gardner ER, Figg WD, Vargesson N (2009). "Thalidomide induces limb defects by preventing angiogenic outgrowth during early limb formation". Proceedings of the National Academy of Sciences of the United States of America. 106 (21): 8573–8. doi:10.1073/pnas.0901505106. PMID 19433787. {{cite journal}}: |access-date= requires |url= (help); Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  39. ^ Search of: pomalidomide - ClinicalTrials.gov
  40. ^ Courtenay-Smith, Natasha (2008-04-23). "A truly special love story: Two married thalidomide survivors living happily 50 years after drug's launch". London: The Daily Mail. Retrieved 2009-06-18.
  41. ^ Orpheus lives: A small good thing in Quastoff Retrieved on 2008-10-22
  42. ^ David Lega personal website

Further reading

  • Stephens, Trent (2001-12-24). Dark Remedy: The Impact of Thalidomide and Its Revival as a Vital Medicine. Perseus. ISBN 0-7382-0590-7. {{cite book}}: Unknown parameter |coauthors= ignored (|author= suggested) (help)
  • Knightley, Phillip (1979). Suffer The Children: The Story of Thalidomide. New York: The Viking Press. ISBN 0-670-68114-8. {{cite book}}: Unknown parameter |coauthors= ignored (|author= suggested) (help)

External links

Retrieved from "https://en.wikipedia.org/w/index.php?title=Thalidomide&oldid=319869169"