He was awarded the Nobel Prize in Medicine in 1943 for joint work with Edward Doisy work in discovering vitamin K and its role in human physiology. Dam's key experiment involved feeding a cholesterol-free diet to chickens. The chickens began hemorrhaging and bleeding uncontrollably after a few weeks. Dam isolated the dietary substance needed for blood clotting and called it the "coagulation vitamin", which became shortened to vitamin K.
He was born and died in Copenhagen.
He received an undergraduate degree in chemistry from the Copenhagen Polytechnic Institute (now the Technical University of Denmark) in 1920, and was appointed as assistant instructor in chemistry at the School of Agriculture and Veterinary Medicine. By 1923 he had attained the post of instructor in biochemistry at Copenhagen University's Physiological Laboratory. He studied microchemistry at the University of Graz under Fritz Pregl in 1925, but returned to Copenhagen University, where he was appointed as an assistant professor at the Institute of Biochemistry in 1928, and assistant professor in 1929. During his time as professor at Copenhagen University he spent some time working abroad, and in 1934 submitted a thesis entitled Nogle Undersøgelser over Sterinernes Biologiske Betydning (Some investigations on the biological significance of the sterines) to Copenhagen University, and received the degree of Ph.D. in biochemistry.
Between 1942–1945 he was a Senior Research Associate at the University of Rochester; it was during this period that he was awarded the 1943 Nobel Prize for Physiology or Medicine.