Pwo DNA polymerase

Identifiers
Symbol	pol

Pwo polymerase is a thermostable DNA polymerase used for the polymerase chain reaction. The abbreviation stands for Pyrococcus woesei, a thermophilic archaeon, from which this polymerase was isolated. This polymerase breaks when reaching erroneous uracil in DNA from the chain extension and, through this readahead function, fewer defective DNA clones are synthesized. It is used much less than the usual Taq or Pfu polymerases.^[1]

References

^ Amir Ghasemi; Ali Hatef Salmanian; Nourkhoda Sadeghifard; Amir Ahmad Salarian; Mohammad Khalifeh Gholi (2011). "Cloning, expression and purification of Pwo polymerase from Pyrococcus woesei". Iran J Microbiol. 3 (3): 118–122. PMC 3279813.

[1] Amir Ghasemi; Ali Hatef Salmanian; Nourkhoda Sadeghifard; Amir Ahmad Salarian; Mohammad Khalifeh Gholi (2011). "Cloning, expression and purification of Pwo polymerase from Pyrococcus woesei". Iran J Microbiol. 3 (3): 118–122. PMC 3279813.

[1]

v t e Enzymes
Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Diffusion-limited enzyme Cofactor Enzyme catalysis
Regulation	Allosteric regulation Cooperativity Enzyme inhibitor Enzyme activator
Classification	EC number Enzyme superfamily Enzyme family List of enzymes
Kinetics	Enzyme kinetics Eadie–Hofstee diagram Hanes–Woolf plot Lineweaver–Burk plot Michaelis–Menten kinetics
Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list) EC7 Translocases (list)