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Ciliate

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This article is about the protozoan phylum Ciliophora. For the type of ciliate cells in general, see Ciliate cells. For the type of leaf margin, see Leaf § Margins (edge).

Ciliates
Temporal range: Ediacaran–Recent
"Ciliata" from Ernst Haeckel's Kunstformen der Natur, 1904
Scientific classification
Domain:
Eukarya
(unranked):
SAR
(unranked):
Alveolata
Phylum:
Ciliophora

Doflein, 1901 emend.
Subphyla and Classes[1]

See text for subclasses.

Synonyms
  • Ciliata Perty, 1852

The ciliates are a group of protozoans characterized by the presence of hair-like organelles called cilia, which are identical in structure to eukaryotic flagella, but are in general shorter and present in much larger numbers, with a different undulating pattern than flagella. Cilia occur in all members of the group (although the peculiar Suctoria only have them for part of the life-cycle) and are variously used in swimming, crawling, attachment, feeding, and sensation. All behavioral patterns are coordinated by signaling processes.[2]

Ciliates are an important group of protists, common almost everywhere there is water — in lakes, ponds, oceans, rivers, and soils. About 3,500 species have been described, and the potential number of extant species is estimated at 30,000.[3] Included in this number are many ectosymbiotic and endosymbiotic species, as well as some obligate and opportunistic parasites. Ciliate species range in size from as little as 10 µm to as much as 4 mm in length, and include some of the most morphologically complex protozoans.[4][5]

In most systems of taxonomy, "Ciliophora" is ranked as a phylum,[6] under either the kingdom Protista[7] or Protozoa.[8] In some systems of classification, ciliated protozoa are placed within the class "Ciliata,"[9][10] (a term which can also refer to an unrelated genus of fish). In the taxonomic scheme proposed by the International Society of Protistologists, which eliminates formal rank designations such as "phylum" and "class", "Ciliophora" is an unranked taxon within Alveolata.[11][12]

Cell structure

Cell structure of a ciliate.

Nuclei

Unlike most other eukaryotes, ciliates have two different sorts of nuclei: a small, diploid micronucleus (reproduction), and a large, polyploid macronucleus (general cell regulation). The latter is generated from the micronucleus by amplification of the genome and heavy editing. The micronucleus serves as the germ line nucleus, but does not express its genes. The macronucleus provides the nuclear RNA for vegetative growth.

Division of the macronucleus occurs by amitosis, and the segregation of the chromosomes occurs by a process whose mechanism is unknown. This process is by no means perfect, and after about 200 generations the cell shows signs of aging. Periodically the macronuclei must be regenerated from the micronuclei. In most, this occurs during conjugation. Here two cells line up, the micronuclei undergo meiosis, some of the haploid daughters are exchanged and then fuse to form new micronuclei and macronuclei.

Cytoplasm

Food vacuoles are formed through phagocytosis and typically follow a particular path through the cell as their contents are digested and broken down by lysosomes so the substances the vacuole contains are then small enough to diffuse through the membrane of the food vacuole into the cell. Anything left in the food vacuole by the time it reaches the cytoproct (anus) is discharged by exocytosis. Most ciliates also have one or more prominent contractile vacuoles, which collect water and expel it from the cell to maintain osmotic pressure, or in some function to maintain ionic balance. In some genera, such as Paramecium, these have a distinctive star shape, with each point being a collecting tube.

Specialized structures in ciliates

Cilia are arranged in rows called kineties. In some forms there are also body polykinetids, for instance, among the spirotrichs where they generally form bristles called cirri. More often body cilia are arranged in mono- and dikinetids, which respectively include one and two kinetosomes (basal bodies), each of which may support a cilium. These are arranged into rows called kineties, which run from the anterior to posterior of the cell. The body and oral kinetids make up the infraciliature, an organization unique to the ciliates and important in their classification, and include various fibrils and microtubules involved in coordinating the cilia.

The infraciliature is one of the main component of the cell cortex. Another are the alveoli, small vesicles under the cell membrane that are packed against it to form a pellicle maintaining the cell's shape, which varies from flexible and contractile to rigid. Numerous mitochondria and extrusomes are also generally present. The presence of alveoli, the structure of the cilia, the form of mitosis and various other details indicate a close relationship between the ciliates, Apicomplexa, and dinoflagellates. These superficially dissimilar groups make up the alveolates.

Feeding

Most ciliates are heterotrophs, feeding on smaller organisms, such as bacteria and algae, and detritus swept into the oral groove (mouth) by modified oral cilia. This usually includes a series of membranelles to the left of the mouth and a paroral membrane to its right, both of which arise from polykinetids, groups of many cilia together with associated structures. The food is moved by the cilia through the mouth pore into the gullet, which forms food vacuoles.

Feeding techniques vary considerably, however. Some ciliates are mouthless and feed by absorption (osmotrophy), while others are predatory and feed on other protozoa and in particular on other ciliates. Some ciliates parasitize animals, although only one species, Balantidium coli, is known to cause disease in humans.[13]

Reproduction and sexual phenomena

Ciliate undergoing the last processes of binary fission, a form of asexual reproduction

Ciliates reproduce asexually, by various kinds of fission.[13] During fission, the micronucleus undergoes mitosis and the macronucleus elongates and undergoes amitosis (except among the Karyorelictean ciliates, whose macronuclei do not divide). The cell then divides in two, and each new cell obtains a copy of the micronucleus and the macronucleus.

Typically, the cell is divided transversally, with the anterior half of the ciliate (the proter) forming one new organism, and the posterior half (the opisthe) forming another. However, other types of fission occur in some ciliate groups. These include budding (the emergence of small ciliated offspring, or "swarmers", from the body of a mature parent); strobilation (multiple divisions along the cell body, producing a chain of new organisms); and palintomy (multiple fissions, usually within a cyst).[13]

Fission may occur spontaneously, as part of the vegetative cell cycle. Alternatively, it may proceed as a result of self-fertilization (autogamy),[14] or it may follow conjugation, a sexual phenomenon in which ciliates of compatible mating types exchange genetic material.

Conjugation

Overview

During conjugation, two cells form a bridge between their cytoplasms, the micronuclei undergo meiosis, the macronuclei disappear, and the haploid micronuclei are exchanged over the bridge. In some ciliates (such as Vorticella), conjugating cells become permanently fused, and one conjugant is absorbed by the other.[15] In most ciliate groups, however, the cells separate after conjugation, and both form new macronuclei from their micronuclei.[9] Conjugation and autogamy are always followed by fission.[13]

More details

In general the process is as follows but the details may vary between species:

  • Compatible mating strains meet and partly fuse.
  • The micronuclei undergo meiosis producing four haploid micronuclei per cell.
  • Three of these micronuclei disintegrate. The fourth undergoes mitosis.
  • The two cells exchange a micronucleus.
  • The cells then separate.
  • The micronuclei in each cell fuse.
  • This is followed by mitosis which occurs three times giving rise to eight micronuclei.
  • Four of the new micronuclei transform into macronuclei
  • Finally binary fission occurs twice yielding four daughter cells.

DNA Rearrangements (gene scrambling)

Development of the Oxytricha macronuclear genome from micronuclear genome

Ciliates contain two types of nuclei: somatic "macronucleus" and the germline "micronucleus". Only the DNA in the micronucleus is passed on during sexual reproduction (conjugation). On the other hand, only the DNA in the macronucleus is actively expressed and results in the phenotype of the organism. Macronuclear DNA is derived from micronuclear DNA by amazingly extensive DNA rearrangement and amplification.

The macronucleus begins as a copy of the micronucleus. The micronuclear chromosomes are fragmented into many smaller pieces and amplified to give many copies. The resulting macronuclear chromosomes often contain only a single gene. In Tetrahymena, the micronucleus has 10 chromosomes (5 per haploid genome), while the macronucleus has over 20,000 chromosomes.[16]

In addition, the micronuclear genes are interrupted by numerous "Internal Eliminated Sequences" (IESs). During development of the macronucleus, IESs are deleted and the remaining gene segments, Macronuclear Destined Sequences (MDSs), are spliced together to give the operational gene. Tetrahymena has about 6000 IESs and about 15% of micronuclear DNA is eliminated during this process. The process is guided by small RNAs and epigenetic chromatin marks.[16]

In spirotrich ciliates (such as Oxytricha), the process is even more complex due to "gene scrambling": the MDSs in the micronucleus are often in different order and orientation from that in the macronuclear gene, and so in addition to deletion, DNA inversion and translocation are required for "unscrambling". This process is guided by long RNAs derived from the parental macronucleus. More than 95% of micronuclear DNA is eliminated during spirotrich macronuclear development.[16]

Fossil record

Until recently, the oldest ciliate fossils known were tintinnids from the Ordovician Period. In 2007, Li et al. published a description of fossil ciliates from the Doushantuo Formation, about 580 million years ago, in the Ediacaran Period. These included two types of tintinnids and a possible ancestral suctorian.[17]

Classification[1]

Further information: Wikispecies:Ciliophora
Stentor roeseli

Subphylum Postciliodesmatophora

Subphylum Intramacronucleata

Oxytricha trifallax

Other

  • Class Mesodiniea (a basal group, possibly separated at Subphylum level)

Some old classifications included Opalinidae in the ciliates.

References

  1. ^ a b Gao, Feng; Warren, Alan; Zhang, Qianqian; Gong, Jun; Miao, Miao; Sun, Ping; Xu, Dapeng; Huang, Jie; Yi, Zhenzhen (2016-04-29). "The All-Data-Based Evolutionary Hypothesis of Ciliated Protists with a Revised Classification of the Phylum Ciliophora (Eukaryota, Alveolata)". Scientific Reports. 6: 24874. doi:10.1038/srep24874. ISSN 2045-2322. PMC 4850378. PMID 27126745.
  2. ^ Witzany, Guenther, Nowacki, Mariusz (ed) (2016). Biocommunication of Ciliates. Springer.ISBN 978-3-319-32209-4
  3. ^ Adl, Sina M., et al. "Diversity, nomenclature, and taxonomy of protists." Systematic Biology 56.4 (2007): 685.
  4. ^ Nielsen, Torkel Gissel, and Thomas Kiørboe. "Regulation of zooplankton biomass and production in a temperate, coastal ecosystem. 2. Ciliates." Limnology and Oceanography 39.3 (1994): 508-519.
  5. ^ Lynn, Denis (2008). The Ciliated Protozoa 3rd Edition. Springer. p. 129. ISBN 978-1-4020-8238-2.
  6. ^ "ITIS Report". Integrated Taxonomic Information System. Retrieved 11 December 2014.
  7. ^ Yi Z, Song W, Clamp JC, Chen Z, Gao S, Zhang Q (December 2008). "Reconsideration of systematic relationships within the order Euplotida (Protista, Ciliophora) using new sequences of the gene coding for small-subunit rRNA and testing the use of combined data sets to construct phylogenies of the Diophrys-complex". Mol. Phylogenet. Evol. 50 (3): 599–607. doi:10.1016/j.ympev.2008.12.006. PMID 19121402.
  8. ^ Miao M, Song W, Chen Z, et al. (2007). "A unique euplotid ciliate, Gastrocirrhus (Protozoa, Ciliophora): assessment of its phylogenetic position inferred from the small subunit rRNA gene sequence". J. Eukaryot. Microbiol. 54 (4): 371–8. doi:10.1111/j.1550-7408.2007.00271.x. PMID 17669163.
  9. ^ a b "Introduction to the Ciliata". Retrieved 2009-01-16.
  10. ^ "Ciliata — Definition from Merriam-Webster's Medical Dictionary". Merriam-Webster. Retrieved 2009-01-16.
  11. ^ Adl, Sina M.; et al. (2005). "The new higher level classification of eukaryotes with emphasis on the taxonomy of protists". Journal of Eukaryotic Microbiology. 52 (5): 399–451. doi:10.1111/j.1550-7408.2005.00053.x. PMID 16248873.
  12. ^ Adl, Sina M.; et al. (2012). "The revised classification of eukaryotes". Journal of Eukaryotic Microbiology. 59 (5): 429–514. doi:10.1111/j.1550-7408.2012.00644.x. PMC 3483872. PMID 23020233.
  13. ^ a b c d Lynn, Denis (2008). The Ciliated Protozoa: Characterization, Classification, and Guide to the Literature (3 ed.). Springer. p. 187. ISBN 978-1-4020-8238-2. 1007/978-1-4020-8239-9
  14. ^ Berger JD (October 1986). "Autogamy in Paramecium. Cell cycle stage-specific commitment to meiosis". Exp. Cell Res. 166 (2): 475–85. doi:10.1016/0014-4827(86)90492-1. PMID 3743667.
  15. ^ Finley, Harold E. "The conjugation of Vorticella microstoma." Transactions of the American Microscopical Society (1943): 97-121.
  16. ^ a b c Mochizuki, Kazufumi (2010). "DNA rearrangements directed by non-coding RNAs in ciliates". Wiley Interdisciplinary Reviews: RNA. 1 (3): 376–387. doi:10.1002/wrna.34. PMC 3746294. PMID 21956937.
  17. ^ Li, C.-W.; et al. (2007). "Ciliated protozoans from the Precambrian Doushantuo Formation, Wengan, South China". Geological Society, London, Special Publications. 286: 151–156. doi:10.1144/SP286.11.
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