Ancient Greek physicians Galen (circa 130–200 ) and Hippocrates (circa 400 BC) and Sumerian clay tablets dating from 2150 BC that advocate the use of similar techniques.
Medieval surgeons Hugo of Lucca, Theoderic of Servia, and his pupil Henri de Mandeville were opponents of Galen's opinion that pus was important to healing, which had led ancient and medieval surgeons to let pus remain in wounds. They advocated draining and cleaning wound lips with wine, dressing the wound after suturing it if necessary, and leaving the dressing on for ten days, soaking it in warm wine all the while, before changing it. Their theories were bitterly opposed by Galenist Guy de Chauliac and others trained in the classical tradition.
Joseph Smith, Jr., alluded to the use of alcohol as an antiseptic in February 1833, when he wrote what is now section 89 of the Doctrine and Covenants, popularly known as the "Word of Wisdom". Specifically, verse 7 states: "And, again, strong drinks are not for the belly, but for the washing of your bodies."
Bacterial growth requires a food supply, moisture, oxygen (if the bacteria are obligate aerobes), and a certain minimum temperature (see bacteriology). These conditions have been studied and dealt with in food preservation and the ancient practice of embalming the dead, which is the earliest known systematic use of antiseptics.
In early inquiries before microbes were understood, much emphasis was given to the prevention of putrefaction, and procedures were carried out to determine the amount of agent that must be added to a given solution to prevent the development of pus and putrefaction; however, due to a lack of a developed understanding of germ theory, this method was inaccurate and, today, an antiseptic is judged by its effect on pure cultures of a defined microbe and/or its vegetative and spore forms. The standardization of antiseptics has been implemented in many instances, and a water solution of phenol of a certain fixed strength is now used as the standard to which other antiseptics are compared.
Boric acid is used in suppositories to treat yeast infections of the vagina, in eyewashes, as an antiviral to shorten the duration of cold sore attacks, in creams for burns, and trace amounts in eye contact solutions.
Brilliant green is a triarylmethane dye still widely used as 1% ethanol solution in Eastern Europe and ex-USSR countries for treatment of small wounds and abscesses. It is efficient against Gram-positive bacteria.
Chlorhexidine gluconate, a biguanidine derivative, is used in concentrations of 0.5–4.0% alone or in lower concentrations in combination with other compounds, such as alcohols as a skin antiseptic and to treat inflammation of the gums (gingivitis). The microbicidal action is somewhat slow, but remanent. It is a cationic surfactant, similar to quats.
Hydrogen peroxide is used as a 6% (20 Vols) solution to clean and deodorize wounds and ulcers. More commonly, 3% solutions of hydrogen peroxide have been used in household first aid for scrapes, etc. However, even this less potent form is no longer recommended for typical wound care, as the strong oxidization causes scar formation and increases healing time. Gentle washing with mild soap and water or rinsing a scrape with sterile saline is a better practice.
Iodine is usually used in an alcohol solution (called tincture of iodine) or as Lugol's iodine solution as a pre- and postoperative antiseptic. Some people do not recommend disinfecting minor wounds with iodine because of concern that it may induce scar tissue formation and increase healing time. However, concentrations of 1% iodine or less have not been shown to increase healing time and are not otherwise distinguishable from treatment with saline. Novel iodine antiseptics containing povidone-iodine (an iodophor, complex of povidone, a water-soluble polymer, with triiodide anions I3-, containing about 10% of active iodine) are far better tolerated, do not negatively affect wound healing, and leave a deposit of active iodine, thereby creating the so-called "remnant", or persistent, effect. The great advantage of iodine antiseptics is their wide scope of antimicrobial activity, killing all principal pathogens and, given enough time, even spores, which are considered to be the most difficult form of microorganisms to be inactivated by disinfectants and antiseptics.
Mercurochrome is not recognized as safe and effective by the US Food and Drug Administration (FDA) due to concerns about its mercury content. Other obsolete organomercury antiseptics include bis-(phenylmercuric) monohydrogenborate (Famosept).
Manuka honey is recognized by the FDA as a medical device for use in wounds and burns. Active +15 is equal to a 15% solution of phenol.
Octenidine dihydrochloride, a cationic surfactant and bis-(dihydropyridinyl)-decane derivative, is used in concentrations of 0.1–2.0%. It is similar in its action to the quats, but is of somewhat broader spectrum of activity. Octenidine is currently increasingly used in continental Europe as a QAC and chlorhexidine (with respect to its slow action and concerns about the carcinogenic impurity 4-chloroaniline) substitute in water- or alcohol-based skin, mucosa, and wound antiseptic. In aqueous formulations, it is often potentiated with addition of 2-phenoxyethanol.
Phenol is germicidal in strong solution, and inhibitory in weaker ones. It is used as a "scrub" for preoperative hand cleansing, and in the form of a powder as an antiseptic baby powder, where it is dusted onto the navel as it heals. Also used in mouthwashes and throat lozenges, it has a painkilling effect, as well as an antiseptic one. Example: TCP. Other phenolic antiseptics include historically important, but today rarely used (sometimes in dental surgery) thymol, today obsolete hexachlorophene, still used triclosan, and sodium 3,5-dibromo-4-hydroxybenzenesulfonate (Dibromol).
Polyhexanide (polyhexamethylene biguanide, PHMB) is an antimicrobial compound suitable for clinical use in critically colonized or infected acute and chronic wounds. The physicochemical action on the bacterial envelope prevents or impedes the development of resistant bacterial strains.
By continued exposure to antibiotics, bacteria may evolve to the point where they are no longer harmed by these compounds.
In contrast, bacteria can develop a resistance to antiseptics, but the effect is generally less pronounced.
The mechanism by which bacteria evolve may vary in response to different antiseptics. Low concentrations of an antiseptic may encourage growth of a bacteria strain that is resistant to the antiseptic, where a higher concentration of the antiseptic would simply kill the bacteria. In addition, use of an excessively high concentration of an antiseptic may cause tissue damage or slow the process of wound healing. Consequently, antiseptics are most effective when used at the correct concentration—a high enough concentration to kill harmful bacteria, fungi or virii, but a low enough concentration to avoid damage to the tissue.
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