D-Man-alpha-(1-3)-D-Glc-beta-(1-4)-D-Glc-alpha-1-diphosphoundecaprenol 2-beta-glucuronyltransferase

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D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol 2-beta-glucuronyltransferase
EC number
IntEnz IntEnz view
ExPASy NiceZyme view
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol 2-beta-glucuronyltransferase (EC, GumK) is an enzyme with systematic name UDP-glucuronate:D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol beta-1,2-glucuronyltransferase.[1][2][3][4][5][6][7] This enzyme catalyses the following chemical reaction

UDP-glucuronate + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol UDP + D-GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol

The enzyme is involved in the biosynthesis of the exopolysaccharides xanthan (in the bacterium Xanthomonas campestris) and acetan (in the bacterium Gluconacetobacter xylinus).


  1. ^ Katzen, F.; Ferreiro, D.U.; Oddo, C.G.; Ielmini, M.V.; Becker, A.; Puhler, A.; Ielpi, L. (1998). "Xanthomonas campestris pv. campestris gum mutants: effects on xanthan biosynthesis and plant virulence". J. Bacteriol. 180 (7): 1607–1617. PMC 107069Freely accessible. PMID 9537354. 
  2. ^ Ielpi, L.; Couso, R.O.; Dankert, M.A. (1993). "Sequential assembly and polymerization of the polyprenol-linked pentasaccharide repeating unit of the xanthan polysaccharide in Xanthomonas campestris". J. Bacteriol. 175 (9): 2490–2500. PMC 204549Freely accessible. PMID 7683019. 
  3. ^ Kim, S.Y.; Kim, J.G.; Lee, B.M.; Cho, J.Y. (2009). "Mutational analysis of the gum gene cluster required for xanthan biosynthesis in Xanthomonas oryzae pv oryzae". Biotechnol. Lett. 31 (2): 265–270. doi:10.1007/s10529-008-9858-3. PMID 18854951. 
  4. ^ Barreras, M.; Bianchet, M.A.; Ielpi, L. (2006). "Crystallization and preliminary crystallographic characterization of GumK, a membrane-associated glucuronosyltransferase from Xanthomonas campestris required for xanthan polysaccharide synthesis". Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 62 (Pt 9): 880–883. doi:10.1107/S1744309106028764. PMC 2242869Freely accessible. PMID 16946469. 
  5. ^ Barreras, M.; Salinas, S.R.; Abdian, P.L.; Kampel, M.A.; Ielpi, L. (2008). "Structure and mechanism of GumK, a membrane-associated glucuronosyltransferase". J. Biol. Chem. 283 (36): 25027–25035. doi:10.1074/jbc.M801227200. PMC 3259810Freely accessible. PMID 18596046. 
  6. ^ Vojnov, A.A.; Bassi, D.E.; Daniels, M.J.; Dankert, M.A. (2002). "Biosynthesis of a substituted cellulose from a mutant strain of Xanthomonas campestris". Carbohydr. Res. 337 (4): 315–326. doi:10.1016/S0008-6215(01)00322-6. PMID 11841812. 
  7. ^ Barreras, M.; Abdian, P.L.; Ielpi, L. (2004). "Functional characterization of GumK, a membrane-associated β-glucuronosyltransferase from Xanthomonas campestris required for xanthan polysaccharide synthesis". Glycobiology. 14 (3): 233–241. doi:10.1093/glycob/cwh056. PMID 14736729. 

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