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TVO is an abbreviation with several different significations:
- Teollisuuden Voima, a Finnish nuclear power company
- Theatre Versus Oppression, a UK registered charity which uses applied theatre techniques
- Total value of ownership, an evaluation method
- Tractor vaporising oil, engine fuel for British tractors
- Total viable organism, a microbiology term for determining the microbial content of a sample
- Televisora de Oriente, a Venezuelan TV station
- TVOntario, a Canadian TV station
- Óptima Televisión, a Chilean TV station
- TV Oberfranken, a regional TV station in Upper Franconia, Germany
- Television Osaka, a Japanese TV station
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