Stryker's Island makes an appearance in Justice League: Crisis on Two Earths. Lex Luthor is shown as an inmate there when Superman uses his heightened vision powers make sure that his Lex Luthor is in Stryker's Island at the time when the alternate Lex Luthor arrived.
Stryker's Island appears in All-Star Superman. Lex Luthor is sentenced to the Death Row section of Stryker's Island. Parasite is also seen as an inmate here.
In the DCEU Stryker's Island is shown to be halfway between Gotham City and Metropolis; it is the location where Doomsday crashed back to Earth after the American government tried to take him out with a nuclear missile when Superman took him into the upper atmosphere.
Stryker's Island is referenced in Mortal Kombat vs. DC Universe. After Darkseid was defeated, Superman grabbed Lex Luthor and says that he will imprison him somewhere that's more secure than Stryker's Island.
In DC Universe Online, Stryker's Island is utilized as both an explorable area and an instanced event. On the map, it is located off the shore of Metropolis, while the instance is reserved for both heroes and villains as a Hard Alert, accessible by either queue or a world map entrance located on the explorable map. The Instance includes battles with Gorilla Grodd, Captain Cold, and Mirror Master amongst others. It is labeled with the "Medium" difficulty indicating it as a part of the upper class of the game's current Hard Alerts (which are measured as either "Normal" or "Medium" in actual difficulty).
Stryker's Island is a stage in Injustice: Gods Among Us. Gorilla Grodd, Parasite, Metallo, and Deadshot all make cameo appearances in the stage's background. In the storyline, Wonder Woman, Green Arrow, Green Lantern, and Aquaman helped the Insurgency's Batman infiltrate Stryker's Island to free the Justice League's Batman. While Batman and Green Arrow infiltrate Stryker's Island, the other pull off a diversion to distract the Regime members present.