The N-ras oncogene is a member of the Ras gene family. It is mapped on chromosome 1, and it is activated in HL60, a promyelocytic leukemia line. The order of nearby genes is as follows: cen—CD2—NGFB—NRAS—tel. The mammalian ras gene family consists of the harvey and kirsten ras genes (HRAS and KRAS), an inactive pseudogene of each (c-Hras2 and c-Kras1) and the N-ras gene. They differ significantly only in the C-terminal 40 amino acids. These ras genes have GTP/GDP binding and GTPase activity, and their normal function may be as G-like regulatory proteins involved in the normal control of cell growth. Mutations which change amino acid residues 12, 13 or 61 activate the potential of N-ras to transform cultured cells and are implicated in a variety of human tumors. The N-ras gene specifies two main transcripts of 2Kb and 4.3Kb. The difference between the two transcripts is a simple extension through the termination site of the 2Kb transcript. The N-ras gene consists of seven exons (-I, I, II, III, IV, V, VI). The smaller 2Kb transcript contains the VIa exon, and the larger 4.3Kb transcript contains the VIb exon which is just a longer form of the VIa exon. Both transcripts encode identical proteins as they differ only the 3' untranslated region. The sequence of the shorter 2Kb transcript is presented here. The 4.3 Kb transcript sequence is not available.
^Marshall CJ, Hall A, Weiss RA (September 1982). "A transforming gene present in human sarcoma cell lines". Nature299 (5879): 171–3. doi:10.1038/299171a0. PMID6287287.
^Shimizu K, Goldfarb M, Perucho M, Wigler M (January 1983). "Isolation and preliminary characterization of the transforming gene of a human neuroblastoma cell line". PNAS80 (2): 383–7. doi:10.1073/pnas.80.2.383. PMID6300838.
van Elsas A, Scheibenbogen C, van der Minne C et al. (1998). "UV-induced N-ras mutations are T-cell targets in human melanoma.". Melanoma Res. 7 Suppl 2: S107–13. PMID9578425.CS1 maint: Explicit use of et al. (link)
Dracopoli NC, Meisler MH (1990). "Mapping the human amylase gene cluster on the proximal short arm of chromosome 1 using a highly informative (CA)n repeat.". Genomics7 (1): 97–102. doi:10.1016/0888-7543(90)90523-W. PMID1692298.
Yuasa Y, Kamiyama T, Kato M et al. (1990). "Transforming genes from familial adenomatous polyposis patient cells detected by a tumorigenicity assay.". Oncogene5 (4): 589–96. PMID1970154.CS1 maint: Explicit use of et al. (link)
Nitta N, Ochiai M, Nagao M, Sugimura T (1987). "Amino-acid substitution at codon 13 of the N-ras oncogene in rectal cancer in a Japanese patient.". Jpn. J. Cancer Res.78 (1): 21–6. PMID3102434.
Raybaud F, Noguchi T, Marics I et al. (1988). "Detection of a low frequency of activated ras genes in human melanomas using a tumorigenicity assay.". Cancer Res.48 (4): 950–3. PMID3276402.CS1 maint: Explicit use of et al. (link)
Hirai H, Kobayashi Y, Mano H et al. (1987). "A point mutation at codon 13 of the N-ras oncogene in myelodysplastic syndrome.". Nature327 (6121): 430–2. doi:10.1038/327430a0. PMID3295562.CS1 maint: Explicit use of et al. (link)
Mitchell EL, Jones D, White GR et al. (1995). "Determination of the gene order of the three loci CD2, NGFB, and NRAS at human chromosome band 1p13 and refinement of their localisation at the subband level by fluorescence in situ hybridisation.". Cytogenet. Cell Genet.70 (3–4): 183–5. doi:10.1159/000134028. PMID7789166.CS1 maint: Explicit use of et al. (link)