Aspartate kinase: Difference between revisions
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'''Aspartate kinase''' |
'''Aspartate kinase''' or '''aspartokinase''' ('''AK''') is an [[enzyme]] that [[catalysis|catalyzes]] the [[phosphorylation]] of the [[amino acid]] [[aspartate]]. This reaction is the first step in the [[biosynthesis]] of three other amino acids: [[methionine]], [[lysine]], and [[threonine]], known as the "aspartate family". Aspartokinases are present only in [[microorganism]]s and [[plant]]s, but not in [[animal]]s, which must obtain aspartate-family amino acids from their [[diet (nutrition)|diet]]. Consequently, methionine, lysine and threonine are [[essential amino acid]]s in animals. |
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==Nomenclature== |
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⚫ | In ''[[Escherichia coli]]'', aspartokinase is present as three independently regulated [[isozyme]]s, each of which is specific to one of the three downstream biochemical pathways. This allows the independent regulation of the rates of methionine, lysine, and threonine production. The forms that produce threonine and lysine are subject to [[enzyme inhibition|feedback inhibition]], and all three can be repressed at the level of [[gene expression]] by high concentrations of their end-products.<ref>{{cite journal | vauthors = Church GM | title = The personal genome project | journal = Molecular Systems Biology | volume = 1 | issue = 1 | pages = 2005.0030 | year = 2004 | pmid = 16729065 | pmc = 1681452 | doi = 10.1038/msb4100040 }}</ref> Absence from animals makes these enzymes key targets for new herbicides and biocides and for improvements in [[nutritional value]] of crops.<ref>{{cite journal | vauthors = Viola RE | title = The central enzymes of the aspartate family of amino acid biosynthesis | journal = Accounts of Chemical Research | volume = 34 | issue = 5 | pages = 339–49 | date = May 2001 | pmid = 11352712 | doi = 10.1021/ar000057q }}</ref> |
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The generic abbreviation for aspartokinases is AK. However, the nomenclature for aspartokinase genes and proteins varies considerable among species. The main aspatokinases are ''[[lysC]]'' (''[[Bacillus subtilis]]'', ''[[Escherichia coli]]'' and many other bacteria), ''ask'' (''[[Mycobacterium bovis]]'', ''[[Thermus thermophilus]]''), ''AK1''–''AK3'' (''[[Arabidopsis thaliana]]''), ''FUB3'' (''[[Fusarium]]'' and ''[[Gibberella]]'') and ''HOM3'' (''[[Saccharomyces cerevisiae]]''). Additionally, ''apk'' is a synonym for ''lysC''.<ref>{{cite book |last1=King |first1=Robert C. |title=Handbook of Genetics: Volume 1 Bacteria, Bacteriophages, and Fungi |date=2013 |publisher=Springer Science & Business Media |isbn=978-1-4899-1710-2 |page=148 |url=https://books.google.es/books?id=lfUHCAAAQBAJ&pg=PA148 |language=en}}</ref> |
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==Enzymatic regulation== |
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Aspartokinases may use the [[morpheein]] model of [[allosteric regulation]].<ref name=pmid22182754>{{cite journal | vauthors = Selwood T, Jaffe EK | title = Dynamic dissociating homo-oligomers and the control of protein function | journal = Archives of Biochemistry and Biophysics | volume = 519 | issue = 2 | pages = 131–43 | date = March 2012 | pmid = 22182754 | pmc = 3298769 | doi = 10.1016/j.abb.2011.11.020 }}</ref> |
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⚫ | In ''[[Escherichia coli]]'', aspartokinase is present as three independently regulated [[isozyme]]s (thrA, metLM and lysC), each of which is specific to one of the three downstream biochemical pathways. This allows the independent regulation of the rates of methionine, lysine, and threonine production. The forms that produce threonine and lysine are subject to [[enzyme inhibition|feedback inhibition]], and all three can be repressed at the level of [[gene expression]] by high concentrations of their end-products.<ref>{{cite journal | vauthors = Church GM | title = The personal genome project | journal = Molecular Systems Biology | volume = 1 | issue = 1 | pages = 2005.0030 | year = 2004 | pmid = 16729065 | pmc = 1681452 | doi = 10.1038/msb4100040 }}</ref> Absence from animals makes these enzymes key targets for new herbicides and biocides and for improvements in [[nutritional value]] of crops.<ref>{{cite journal | vauthors = Viola RE | title = The central enzymes of the aspartate family of amino acid biosynthesis | journal = Accounts of Chemical Research | volume = 34 | issue = 5 | pages = 339–49 | date = May 2001 | pmid = 11352712 | doi = 10.1021/ar000057q }}</ref> |
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== References == |
== References == |
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{{Enzymes}} |
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[[Category:EC 2.7.2]] |
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Revision as of 15:49, 11 August 2020
Aspartate kinase | |||||||||
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Identifiers | |||||||||
EC no. | 2.7.2.4 | ||||||||
CAS no. | 9012-50-4 | ||||||||
Databases | |||||||||
IntEnz | IntEnz view | ||||||||
BRENDA | BRENDA entry | ||||||||
ExPASy | NiceZyme view | ||||||||
KEGG | KEGG entry | ||||||||
MetaCyc | metabolic pathway | ||||||||
PRIAM | profile | ||||||||
PDB structures | RCSB PDB PDBe PDBsum | ||||||||
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Aspartate kinase or aspartokinase (AK) is an enzyme that catalyzes the phosphorylation of the amino acid aspartate. This reaction is the first step in the biosynthesis of three other amino acids: methionine, lysine, and threonine, known as the "aspartate family". Aspartokinases are present only in microorganisms and plants, but not in animals, which must obtain aspartate-family amino acids from their diet. Consequently, methionine, lysine and threonine are essential amino acids in animals.
Nomenclature
The generic abbreviation for aspartokinases is AK. However, the nomenclature for aspartokinase genes and proteins varies considerable among species. The main aspatokinases are lysC (Bacillus subtilis, Escherichia coli and many other bacteria), ask (Mycobacterium bovis, Thermus thermophilus), AK1–AK3 (Arabidopsis thaliana), FUB3 (Fusarium and Gibberella) and HOM3 (Saccharomyces cerevisiae). Additionally, apk is a synonym for lysC.[1]
Enzymatic regulation
Aspartokinases may use the morpheein model of allosteric regulation.[2]
In Escherichia coli, aspartokinase is present as three independently regulated isozymes (thrA, metLM and lysC), each of which is specific to one of the three downstream biochemical pathways. This allows the independent regulation of the rates of methionine, lysine, and threonine production. The forms that produce threonine and lysine are subject to feedback inhibition, and all three can be repressed at the level of gene expression by high concentrations of their end-products.[3] Absence from animals makes these enzymes key targets for new herbicides and biocides and for improvements in nutritional value of crops.[4]
References
- ^ King, Robert C. (2013). Handbook of Genetics: Volume 1 Bacteria, Bacteriophages, and Fungi. Springer Science & Business Media. p. 148. ISBN 978-1-4899-1710-2.
- ^ Selwood T, Jaffe EK (March 2012). "Dynamic dissociating homo-oligomers and the control of protein function". Archives of Biochemistry and Biophysics. 519 (2): 131–43. doi:10.1016/j.abb.2011.11.020. PMC 3298769. PMID 22182754.
- ^ Church GM (2004). "The personal genome project". Molecular Systems Biology. 1 (1): 2005.0030. doi:10.1038/msb4100040. PMC 1681452. PMID 16729065.
- ^ Viola RE (May 2001). "The central enzymes of the aspartate family of amino acid biosynthesis". Accounts of Chemical Research. 34 (5): 339–49. doi:10.1021/ar000057q. PMID 11352712.
External links
- EC 2.7.2.4
- Aspartokinase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)