DNA polymerase V

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DNA polymerase V, subunit C
Identifiers
Organism Escherichia coli
(str. K-12 substr. MG1655)
Symbol umuC
Entrez 946359
RefSeq (Prot) NP_415702.1
UniProt P04152
Other data
EC number 2.7.7.7
Chromosome genome: 1.23 - 1.23 Mb
DNA polymerase V, subunit D
Identifiers
Organism Escherichia coli
(str. K-12 substr. MG1655)
Symbol umuD
Entrez 945746
RefSeq (Prot) NP_415701.1
UniProt P0AG11
Other data
EC number 3.4.21.-
Chromosome genome: 1.23 - 1.23 Mb

DNA polymerase V is a prokaryotic polymerase. that is involved in SOS response and translesion synthesis DNA repair mechanisms.It is related to DNA Polymerase IV, being both of them Y-family DNA polymerases that are involved in SOS response and translesion synthesis DNA repair mechanisms. Transcription of Pol V via the umuDC genes is highly regulated to produce only Pol V when damaged DNA is present in the cell generating an SOS response. Stalled polymerases causes RecA to bind to the ssDNA, which causes the LexA protein to autodigest. LexA then loses is ability to repress the transcription of the umuDC operon. The same RecA-ssDNA nucleoprotein posttranslationally modifies the UmuD protein into UmuD' protein. ≈UmuD and UmuD' form a heterodimer that interacts with UmuC, which in turn activates umuC's polymerase catalytic activity on damaged DNA.[1][2]

References[edit]

  1. ^ Pham P, Rangarajan S, Woodgate R, Goodman MF (July 2001). "Roles of DNA polymerases V and II in SOS-induced error-prone and error-free repair in Escherichia coli". Proc. Natl. Acad. Sci. U.S.A. 98 (15): 8350–4. doi:10.1073/pnas.111007198. PMC 37442. PMID 11459974. 
  2. ^ Gonzalez M, Woodgate R (February 2002). "The "tale" of UmuD and its role in SOS mutagenesis". Bioessays 24 (2): 141–8. doi:10.1002/bies.10040. PMID 11835278.