UBE2N

Ubiquitin-conjugating enzyme E2N
PDB rendering based on 1j7d.
Available structures PDB 1J7D, 2C2V, 3HCT, 3HCU
Identifiers
Symbols	UBE2N; MGC131857; MGC8489; UBC13; UbcH-ben
External IDs	OMIM: 603679 MGI: 1934835 HomoloGene: 100734 GeneCards: UBE2N Gene
EC number	6.3.2.19
Gene Ontology Molecular function • nucleotide binding • ubiquitin-protein ligase activity • protein binding • ATP binding • ligase activity • acid-amino acid ligase activity • ubiquitin binding Cellular component • ubiquitin ligase complex • nucleus • cytoplasm • cytoplasm • cytosol • cytosol • UBC13-MMS2 complex • UBC13-UEV1A complex Biological process • double-strand break repair via homologous recombination • DNA double-strand break processing • toll-like receptor signaling pathway • MyD88-dependent toll-like receptor signaling pathway • MyD88-dependent toll-like receptor signaling pathway • regulation of DNA repair • postreplication repair • protein modification process • proteolysis • Toll signaling pathway • protein ubiquitination • protein ubiquitination • histone ubiquitination • positive regulation of histone modification • regulation of histone ubiquitination • toll-like receptor 1 signaling pathway • toll-like receptor 2 signaling pathway • toll-like receptor 4 signaling pathway • positive regulation of I-kappaB kinase/NF-kappaB cascade • positive regulation of I-kappaB kinase/NF-kappaB cascade • innate immune response • positive regulation of DNA repair • T cell receptor signaling pathway • T cell receptor signaling pathway • positive regulation of NF-kappaB transcription factor activity • positive regulation of ubiquitin-protein ligase activity • protein K63-linked ubiquitination Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	7334	93765
Ensembl	ENSG00000177889	ENSMUSG00000074781
UniProt	P61088	P61089
RefSeq (mRNA)	NM_003348	NM_080560.3
RefSeq (protein)	NP_003339	NP_542127.1
Location (UCSC)	Chr 12: 93.8 – 93.84 Mb	Chr 10: 94.98 – 95.01 Mb
PubMed search	[1]	[2]

Ubiquitin-conjugating enzyme E2 N is a protein that in humans is encoded by the UBE2N gene.

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair.

Interactions

UBE2N has been shown to interact with Aurora A kinase, TRAF2, TRAF6, HLTF and UBE2V1.

Further reading

• Bonaldo MF, Lennon G, Soares MB (1997). "Normalization and subtraction: two approaches to facilitate gene discovery.". Genome Res. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548. 
• Hofmann RM, Pickart CM (1999). "Noncanonical MMS2-encoded ubiquitin-conjugating enzyme functions in assembly of novel polyubiquitin chains for DNA repair.". Cell 96 (5): 645–53. doi:10.1016/S0092-8674(00)80575-9. PMID 10089880. 
• Deng L, Wang C, Spencer E, et al. (2000). "Activation of the IkappaB kinase complex by TRAF6 requires a dimeric ubiquitin-conjugating enzyme complex and a unique polyubiquitin chain.". Cell 103 (2): 351–61. doi:10.1016/S0092-8674(00)00126-4. PMID 11057907. 
• Chan NL, Hill CP (2001). "Defining polyubiquitin chain topology.". Nat. Struct. Biol. 8 (8): 650–2. doi:10.1038/90337. PMID 11473244. 
• Moraes TF, Edwards RA, McKenna S, et al. (2001). "Crystal structure of the human ubiquitin conjugating enzyme complex, hMms2-hUbc13.". Nat. Struct. Biol. 8 (8): 669–73. doi:10.1038/90373. PMID 11473255. 
• Ashley C, Pastushok L, McKenna S, et al. (2002). "Roles of mouse UBC13 in DNA postreplication repair and Lys63-linked ubiquitination.". Gene 285 (1-2): 183–91. doi:10.1016/S0378-1119(02)00409-2. PMID 12039045. 
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241. 
• McKenna S, Moraes T, Pastushok L, et al. (2003). "An NMR-based model of the ubiquitin-bound human ubiquitin conjugation complex Mms2.Ubc13. The structural basis for lysine 63 chain catalysis.". J. Biol. Chem. 278 (15): 13151–8. doi:10.1074/jbc.M212353200. PMID 12569095. 
• Anandasabapathy N, Ford GS, Bloom D, et al. (2003). "GRAIL: an E3 ubiquitin ligase that inhibits cytokine gene transcription is expressed in anergic CD4+ T cells.". Immunity 18 (4): 535–47. doi:10.1016/S1074-7613(03)00084-0. PMID 12705856. 
• Ewart-Toland A, Briassouli P, de Koning JP, et al. (2003). "Identification of Stk6/STK15 as a candidate low-penetrance tumor-susceptibility gene in mouse and human.". Nat. Genet. 34 (4): 403–12. doi:10.1038/ng1220. PMID 12881723. 
• Bothos J, Summers MK, Venere M, et al. (2003). "The Chfr mitotic checkpoint protein functions with Ubc13-Mms2 to form Lys63-linked polyubiquitin chains.". Oncogene 22 (46): 7101–7. doi:10.1038/sj.onc.1206831. PMID 14562038. 
• Zhou H, Wertz I, O'Rourke K, et al. (2004). "Bcl10 activates the NF-kappaB pathway through ubiquitination of NEMO.". Nature 427 (6970): 167–71. doi:10.1038/nature02273. PMID 14695475. 
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs.". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039. 
• Sun L, Deng L, Ea CK, et al. (2004). "The TRAF6 ubiquitin ligase and TAK1 kinase mediate IKK activation by BCL10 and MALT1 in T lymphocytes.". Mol. Cell 14 (3): 289–301. doi:10.1016/S1097-2765(04)00236-9. PMID 15125833. 
• Colland F, Jacq X, Trouplin V, et al. (2004). "Functional proteomics mapping of a human signaling pathway.". Genome Res. 14 (7): 1324–32. doi:10.1101/gr.2334104. PMC 442148. PMID 15231748. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=442148. 
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528928. 
• Pastushok L, Moraes TF, Ellison MJ, Xiao W (2005). "A single Mms2 "key" residue insertion into a Ubc13 pocket determines the interface specificity of a human Lys63 ubiquitin conjugation complex.". J. Biol. Chem. 280 (18): 17891–900. doi:10.1074/jbc.M410469200. PMID 15749714. 
• Takeuchi T, Yokosawa H (2005). "ISG15 modification of Ubc13 suppresses its ubiquitin-conjugating activity.". Biochem. Biophys. Res. Commun. 336 (1): 9–13. doi:10.1016/j.bbrc.2005.08.034. PMID 16112642. 
• Zou W, Papov V, Malakhova O, et al. (2005). "ISG15 modification of ubiquitin E2 Ubc13 disrupts its ability to form thioester bond with ubiquitin.". Biochem. Biophys. Res. Commun. 336 (1): 61–8. doi:10.1016/j.bbrc.2005.08.038. PMID 16122702.