Ubiquitin-activating enzyme

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Ubiquitin—protein ligase
Ubiquitin-activating enzyme bound to Ubiquitin.png
Crystal structure of the yeast ubiquitin-activating enzyme E1 / ubiquitin complex.[1]
Identifiers
EC number 6.3.2.19
CAS number 74812-49-0
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum
Gene Ontology AmiGO / EGO

Ubiquitin-activating enzymes, also known as E1 enzymes, catalyze the first step in the ubiquitination reaction, which (among other things) can target a protein for degradation via a proteasome. This covalent attachment of ubiquitin or ubiquitin-like proteins to targeted proteins is a major mechanism for regulating protein function in eukaryotic organisms.[2] Many processes such as cell division, immune responses and embryonic development are also regulated by post-transcriptional modification by ubiquitin and ubiquitin-like proteins.[2]

Overview of ubiquitination (ubiquitylation)[edit]

Ubiquitin-activating enzyme (E1) starts the ubiquitination process (Figure 1). The E1 enzyme along with ATP binds to the ubiquitin protein. The E1 enzyme then passes the ubiquitin protein to a second protein, called Ubiquitin carrier or conjugation protein (E2). The E2 protein complexes with an Ubiquitin protein ligase (E3). This Ubiquitin protein ligase recognizes which protein needs to be tagged and catalyzes the transfer of ubiquitin to that protein. This pathway repeats itself until the target protein has a full chain of ubiquitin attached to itself.[3]

Structure and mechanism[edit]

At the start of the ubiquitination cascade, the E1 enzyme (Figure 2) binds ATP-Mg2+ and ubiquitin and catalyses ubiquitin C-terminal acyl adenylation.[4] In the next step a catalytic cysteine (Figure 3) on the E1 enzyme attacks the ubiquitin-AMP complex through acyl substitution, simultaneously creating a thioester bond and an AMP leaving group.[2] Finally, the E1~ubiquitin complex transfers ubiquitin to an E2 enzyme through a transthioesterification reaction, in which an E2 catalytic cysteine attacks the backside of the E1~ubiquitin complex.[5] However, the transthioesterification process is very complicated, as both E1 and E2 enzymes form an intermediate complex wherein both enzymes undergo a series of conformational changes in order to bind with one another.[5]

Throughout this mechanism, the E1 enzyme is bound to two ubiquitin molecules. Although this secondary ubiquitin is similarly adenylated, it does not form the same thioester complex described previously. The function of the secondary ubiquitin remains largely unknown, however it is believed that it may facilitate conformational changes seen in the E1 enzyme during the transthioesterification process.[2]

Figure 1. shows the sequence of how the ubiquitin activating enzyme attaches to ATP and the ubiquitin substrate. It also shows how two ubiquitin substrates can be bound at one time.[6] 
Figure 2. E1 protein binds a molecule of ubiquitin in each of two identical active sites (highlighted). The important residues, Cysteine and Arginine, are labeled in red.[2] 
Figure 3. Close-up view of the unbound active site. Arg (603) is believed to recharge the catalytic Cys (600) once ubiquitin has been transferred to the E2 enzyme.[2] 
Figure 4. Full mechanism for adenylation of ubiquitin and subsequent ubiquitin binding to E1. 
Schematic diagram of the ubiquitylation system. 

Isozymes[edit]

The following human genes encode ubiquitin-activating enzymes:

Disease association[edit]

The ubiquitin-proteasome system is critical to appropriate protein degradation within cells. Dysfunctions of this system can disrupt cellular homeostasis and lead to a host of disorders. In normally functioning cells, the covalent linkage of ubiquitin or ubiquitin-like protein to a target protein changes the target protein’s surface. These ubiquitinylated proteins are subject to degradation by proteolytic and non-proteolytic pathways.[7] If this system malfunctions, numerous inherited and acquired diseases may result, such as cancer, diabetes, stroke, Alzheimer’s disease, amyotropic lateral sclerosis, multiple sclerosis, asthma, inflammatory bowel disease, autoimmune thyroiditis, inflammatory arthritis and lupus.[7]

Missense in UBE1 and X-linked infantile spinal muscular atrophy (XL-SMA)[edit]

Among the various disorders associated with the ubiquitin-proteasome pathway is X-linked infantile spinal muscular atrophy (XL-SMA).[8] The fatal childhood disorder is associated with loss of anterior horn cells and infantile death. Clinical features include hypotonia, areflexia, and multiple congenital contractures. In a large-scale mutation analysis, screening of six XL-SMA families provided results indicating two novel missense mutations in two families and a novel synonymous C->T substitution in another three families[footnote]. All of these detected mutations were located in exon 15 of the UBE1 gene (the gene encoding ubiquitin-activating enzyme) and were observed to segregate with disease in the families. In brevity, UBE1 missense may lead to a disturbed complex building with gigaxonin, a protein involved in axonal structure and neuronal maintenance. This can lead to impaired degradation of microtubule-associated protein 1B (MAP1B), resulting in the build-up of MAP1B protein, which may enhance neuronal cell death.[8] Thus, mutations in UBE1 are suspected to be the cause of genetic defects in XL-SMA individuals.

References[edit]

  1. ^ PDB 3CMM; Lee I, Schindelin H (July 2008). "Structural insights into E1-catalyzed ubiquitin activation and transfer to conjugating enzymes". Cell 134 (2): 268–78. doi:10.1016/j.cell.2008.05.046. PMID 18662542. 
  2. ^ a b c d e f Schulman BA, Harper JW (May 2009). "Ubiquitin-like protein activation by E1 enzymes: the apex for downstream signalling pathways". Nature Reviews Molecular Cell Biology 10 (5): 319–31. doi:10.1038/nrm2673. PMC 2712597. PMID 19352404. 
  3. ^ Lecker SH, Goldberg AL, Mitch WE (July 2006). "Protein degradation by the ubiquitin-proteasome pathway in normal and disease states". Journal of the American Society of Nephrology : JASN 17 (7): 1807–19. doi:10.1681/ASN.2006010083. PMID 16738015. 
  4. ^ Tokgöz Z, Bohnsack RN, Haas AL (May 2006). "Pleiotropic effects of ATP.Mg2+ binding in the catalytic cycle of ubiquitin-activating enzyme". The Journal of Biological Chemistry 281 (21): 14729–37. doi:10.1074/jbc.M513562200. PMID 16595681. 
  5. ^ a b Lee I, Schindelin H (July 2008). "Structural insights into E1-catalyzed ubiquitin activation and transfer to conjugating enzymes". Cell 134 (2): 268–78. doi:10.1016/j.cell.2008.05.046. PMID 18662542. 
  6. ^ This figure is adapted from Scheme 1 of "The Mechanism of Ubiquitin Activating Enzyme: A Kinetic and Equilbrium Analysis" by Arthur Haas. Haas AL, Rose IA (September 1982). "The mechanism of ubiquitin activating enzyme. A kinetic and equilibrium analysis". The Journal of Biological Chemistry 257 (17): 10329–37. PMID 6286650. 
  7. ^ a b Wang J, Maldonado MA (August 2006). "The ubiquitin-proteasome system and its role in inflammatory and autoimmune diseases". Cellular & Molecular Immunology 3 (4): 255–61. PMID 16978533. 
  8. ^ a b Ramser J, Ahearn ME, Lenski C, et al. (January 2008). "Rare Missense and Synonymous Variants in UBE1 Are Associated with X-Linked Infantile Spinal Muscular Atrophy". American Journal of Human Genetics 82 (1): 188–93. doi:10.1016/j.ajhg.2007.09.009. PMC 2253959. PMID 18179898. 

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