DNAJB6

DNAJB6
Identifiers
Aliases	DNAJB6, Dnajb6, Mrj, mDj4, DJ4, DnaJ, HHDJ1, HSJ-2, HSJ2, LGMD1D, LGMD1E, MSJ-1, DnaJ heat shock protein family (Hsp40) member B6, LGMDD1
External IDs	OMIM: 611332 MGI: 1344381 HomoloGene: 38058 GeneCards: DNAJB6
Gene location (Human)
Chr.	Chromosome 7 (human)
End	157,417,439 bp
Gene location (Mouse)
Chr.	Chromosome 5 (mouse)
End	30,023,132 bp
RNA expression pattern
	Top expressed in
	ganglionic eminence; ; gastrocnemius muscle; ; skin of abdomen; ; popliteal artery; ; monocyte; ; ascending aorta; ; right coronary artery; ; left coronary artery; ; cerebellar hemisphere; ; right lung;
	Top expressed in
	spermatocyte; ; spermatid; ; morula; ; superior frontal gyrus; ; seminiferous tubule; ; yolk sac; ; lip; ; secondary oocyte; ; thymus; ; cerebellar cortex;
	More reference expression data
	; ;
	More reference expression data
Gene ontology
Molecular function	chaperone binding; heat shock protein binding; unfolded protein binding; protein binding; ATPase activator activity; identical protein binding; DNA binding;
Cellular component	cytoplasm; cytosol; membrane; nucleoplasm; Z disc; perinuclear region of cytoplasm; nucleus;
Biological process	intermediate filament organization; negative regulation of cysteine-type endopeptidase activity involved in apoptotic process; negative regulation of inclusion body assembly; regulation of protein localization; protein folding; regulation of cellular response to heat; positive regulation of ATP-dependent activity; actin cytoskeleton organization; extracellular matrix organization; protein localization to nucleus; negative regulation of transcription, DNA-templated; chorio-allantoic fusion; syncytiotrophoblast cell differentiation involved in labyrinthine layer development; chorion development;
	Sources:Amigo / QuickGO
Orthologs
	10049
	23950
	ENSG00000105993
	ENSMUSG00000029131
	O75190
	O54946
	NM_005494; NM_058246; NM_001363676
NM_001037940; NM_001037941; NM_001127367; NM_011847; NM_001359198;
	NM_001378835
	NP_005485; NP_490647; NP_001350605
NP_001033029; NP_001033030; NP_001120839; NP_035977; NP_001346127;
	NP_001365764
	Wikidata
View/Edit Human	View/Edit Mouse

DnaJ homolog subfamily B member 6 is a protein that in humans is encoded by the DNAJB6 gene.^[5]^[6]^[7]

Function

This gene encodes a member of the DNAJ protein family. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. This family member may also play a role in polyglutamine aggregation in specific neurons. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been fully described.^[7]

Interactions

DNAJB6 has been shown to interact with Keratin 18.^[8] It has been also shown that the aggregation of Aβ42 (a process involved in e.g. Alzheimer's disease) is retarded by DNAJB6 in a concentration-dependent manner, extending to very low sub-stoichiometric molar ratios of chaperone to peptide.^[9]

References

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000105993 – Ensembl, May 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000029131 – Ensembl, May 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Seki N, Hattori A, Hayashi A, Kozuma S, Miyajima N, Saito T (June 1999). "Cloning, tissue expression, and chromosomal assignment of human MRJ gene for a member of the DNAJ protein family". J Hum Genet. 44 (3): 185–9. doi:10.1007/s100380050139. PMID 10319584.
^ Pei L (March 1999). "Pituitary tumor-transforming gene protein associates with ribosomal protein S10 and a novel human homologue of DnaJ in testicular cells". J Biol Chem. 274 (5): 3151–8. doi:10.1074/jbc.274.5.3151. PMID 9915854.{{cite journal}}: CS1 maint: unflagged free DOI (link)
^ ^a ^b "Entrez Gene: DNAJB6 DnaJ (Hsp40) homolog, subfamily B, member 6".
^ Izawa I, Nishizawa M, Ohtakara K, Ohtsuka K, Inada H, Inagaki M (2000). "Identification of Mrj, a DnaJ/Hsp40 family protein, as a keratin 8/18 filament regulatory protein". J. Biol. Chem. 275 (44): 34521–7. doi:10.1074/jbc.M003492200. PMID 10954706.{{cite journal}}: CS1 maint: unflagged free DOI (link)
^ Månsson, C; Arosio, P; Hussein, R; Kampinga, H H; Hashem, R M; Boelens, W C; Emanuelsson, C (2014). "Interaction of the molecular chaperone DNAJB6 with growing amyloid-beta 42 (Aβ42) aggregates leads to sub-stoichiometric inhibition of amyloid formation". J. Biol. Chem. 289 (45): 31066–76. doi:10.1074/jbc.M114.595124. PMC 4223311. PMID 25217638.{{cite journal}}: CS1 maint: unflagged free DOI (link)

Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
Suzuki Y; Yoshitomo-Nakagawa K; Maruyama K; et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149. {{cite journal}}: Unknown parameter |name-list-format= ignored (|name-list-style= suggested) (help)
Izawa I; Nishizawa M; Ohtakara K; et al. (2000). "Identification of Mrj, a DnaJ/Hsp40 family protein, as a keratin 8/18 filament regulatory protein". J. Biol. Chem. 275 (44): 34521–7. doi:10.1074/jbc.M003492200. PMID 10954706. {{cite journal}}: Unknown parameter |name-list-format= ignored (|name-list-style= suggested) (help)CS1 maint: unflagged free DOI (link)
Hartley JL, Temple GF, Brasch MA (2001). "DNA Cloning Using In Vitro Site-Specific Recombination". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863.
Ohtsuka K, Hata M (2001). "Mammalian HSP40/DNAJ homologs: cloning of novel cDNAs and a proposal for their classification and nomenclature". Cell Stress Chaperones. 5 (2): 98–112. doi:10.1379/1466-1268(2000)005<0098:MHDHCO>2.0.CO;2. ISSN 1466-1268. PMC 312896. PMID 11147971.
Wiemann S; Weil B; Wellenreuther R; et al. (2001). "Toward a Catalog of Human Genes and Proteins: Sequencing and Analysis of 500 Novel Complete Protein Coding Human cDNAs". Genome Res. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166. {{cite journal}}: Unknown parameter |name-list-format= ignored (|name-list-style= suggested) (help)
Chuang JZ; Zhou H; Zhu M; et al. (2002). "Characterization of a brain-enriched chaperone, MRJ, that inhibits Huntingtin aggregation and toxicity independently". J. Biol. Chem. 277 (22): 19831–8. doi:10.1074/jbc.M109613200. PMID 11896048. {{cite journal}}: Unknown parameter |name-list-format= ignored (|name-list-style= suggested) (help)CS1 maint: unflagged free DOI (link)
Farinha CM; Nogueira P; Mendes F; et al. (2002). "The human DnaJ homologue (Hdj)-1/heat-shock protein (Hsp) 40 co-chaperone is required for the in vivo stabilization of the cystic fibrosis transmembrane conductance regulator by Hsp70". Biochem. J. 366 (Pt 3): 797–806. doi:10.1042/BJ20011717. PMC 1222832. PMID 12069690. {{cite journal}}: Unknown parameter |name-list-format= ignored (|name-list-style= suggested) (help)
Strausberg RL; Feingold EA; Grouse LH; et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. {{cite journal}}: Unknown parameter |name-list-format= ignored (|name-list-style= suggested) (help)
Scherer SW; Cheung J; MacDonald JR; et al. (2003). "Human Chromosome 7: DNA Sequence and Biology". Science. 300 (5620): 767–72. doi:10.1126/science.1083423. PMC 2882961. PMID 12690205. {{cite journal}}: Unknown parameter |name-list-format= ignored (|name-list-style= suggested) (help)
Hillier LW; Fulton RS; Fulton LA; et al. (2003). "The DNA sequence of human chromosome 7". Nature. 424 (6945): 157–64. doi:10.1038/nature01782. PMID 12853948. {{cite journal}}: Unknown parameter |name-list-format= ignored (|name-list-style= suggested) (help)
Hanai R, Mashima K (2004). "Characterization of two isoforms of a human DnaJ homologue, HSJ2". Mol. Biol. Rep. 30 (3): 149–53. doi:10.1023/A:1024916223616. PMID 12974469.
Ota T; Suzuki Y; Nishikawa T; et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039. {{cite journal}}: Unknown parameter |name-list-format= ignored (|name-list-style= suggested) (help)
Liu Y; Zhu MC; Wang YJ; et al. (2004). "[Cloning of a DnaJ homolog chaperon PBP and its subcellular localization]". Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 19 (6): 531–4. PMID 15182641. {{cite journal}}: Unknown parameter |name-list-format= ignored (|name-list-style= suggested) (help)
Berruti G, Martegani E (2005). "The deubiquitinating enzyme mUBPy interacts with the sperm-specific molecular chaperone MSJ-1: the relation with the proteasome, acrosome, and centrosome in mouse male germ cells". Biol. Reprod. 72 (1): 14–21. doi:10.1095/biolreprod.104.030866. PMID 15342353.
Gerhard DS; Wagner L; Feingold EA; et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. {{cite journal}}: Unknown parameter |name-list-format= ignored (|name-list-style= suggested) (help)
Wiemann S; Arlt D; Huber W; et al. (2004). "From ORFeome to Biology: A Functional Genomics Pipeline". Genome Res. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC 528930. PMID 15489336. {{cite journal}}: Unknown parameter |name-list-format= ignored (|name-list-style= suggested) (help)
Rual JF; Venkatesan K; Hao T; et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514. {{cite journal}}: Unknown parameter |name-list-format= ignored (|name-list-style= suggested) (help)
Dai YS, Xu J, Molkentin JD (2005). "The DnaJ-Related Factor Mrj Interacts with Nuclear Factor of Activated T Cells c3 and Mediates Transcriptional Repression through Class II Histone Deacetylase Recruitment". Mol. Cell. Biol. 25 (22): 9936–48. doi:10.1128/MCB.25.22.9936-9948.2005. PMC 1280278. PMID 16260608.

External links

DNAJB6 human gene location in the UCSC Genome Browser.
DNAJB6 human gene details in the UCSC Genome Browser.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000105993 – Ensembl, May 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000029131 – Ensembl, May 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid10319584-5] Seki N, Hattori A, Hayashi A, Kozuma S, Miyajima N, Saito T (June 1999). "Cloning, tissue expression, and chromosomal assignment of human MRJ gene for a member of the DNAJ protein family". J Hum Genet. 44 (3): 185–9. doi:10.1007/s100380050139. PMID 10319584.

[pmid9915854-6] Pei L (March 1999). "Pituitary tumor-transforming gene protein associates with ribosomal protein S10 and a novel human homologue of DnaJ in testicular cells". J Biol Chem. 274 (5): 3151–8. doi:10.1074/jbc.274.5.3151. PMID 9915854.{{cite journal}}: CS1 maint: unflagged free DOI (link)

[entrez-7] "Entrez Gene: DNAJB6 DnaJ (Hsp40) homolog, subfamily B, member 6".

[pmid10954706-8] Izawa I, Nishizawa M, Ohtakara K, Ohtsuka K, Inada H, Inagaki M (2000). "Identification of Mrj, a DnaJ/Hsp40 family protein, as a keratin 8/18 filament regulatory protein". J. Biol. Chem. 275 (44): 34521–7. doi:10.1074/jbc.M003492200. PMID 10954706.{{cite journal}}: CS1 maint: unflagged free DOI (link)

[pmid25217638-9] Månsson, C; Arosio, P; Hussein, R; Kampinga, H H; Hashem, R M; Boelens, W C; Emanuelsson, C (2014). "Interaction of the molecular chaperone DNAJB6 with growing amyloid-beta 42 (Aβ42) aggregates leads to sub-stoichiometric inhibition of amyloid formation". J. Biol. Chem. 289 (45): 31066–76. doi:10.1074/jbc.M114.595124. PMC 4223311. PMID 25217638.{{cite journal}}: CS1 maint: unflagged free DOI (link)

[1]

[2]

[3]

[4]

[5]

[6]

[7]

[8]

[9]

Function

Interactions

References

Further reading

External links