It is commonly available as an over-the-counter substance in various dosage forms, such as a cream, and also (especially in the case of ear infection) as a combination medicine. It is also available as a troche or throat lozenge (prescription only). For ear infection, it is often applied in liquid form, as ear drops.
Clotrimazole is also commonly used in conjunction with betamethasone. Additionally, clotrimazole is used to treat the sickling of cells (related to sickle cell anemia) by blocking ion channels or clocip B channels in the red blood cell membrane, keeping ions and water within the cell.
The primary mechanism of action of clotrimazole is against the division and growing of fungi. Clotrimazole alters the permeability of the fungal cell wall and inhibits the activity of enzymes within the cell. It specifically inhibits the biosynthesis of ergosterol and other sterols required for cell membrane production. Studies show minimal concentrations of clotrimazole cause leakage of intracellular phosphorus compounds into the ambient medium, along with the breakdown of cellular nucleic acids and an accelerated K+efflux. This leads eventually to the cell's death. It does not appreciably spread through the user's body, but remains at the point of application.