SLC5A1

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Solute carrier family 5 (sodium/glucose cotransporter), member 1
Identifiers
Symbols SLC5A1 ; D22S675; NAGT; SGLT1
External IDs OMIM182380 MGI107678 HomoloGene55456 ChEMBL: 4979 GeneCards: SLC5A1 Gene
RNA expression pattern
PBB GE SLC5A1 206628 at tn.png
More reference expression data
Orthologs
Species Human Mouse
Entrez 6523 20537
Ensembl ENSG00000100170 ENSMUSG00000011034
UniProt P13866 Q8C3K6
RefSeq (mRNA) NM_000343 NM_019810
RefSeq (protein) NP_000334 NP_062784
Location (UCSC) Chr 22:
32.44 – 32.51 Mb
Chr 5:
33.1 – 33.16 Mb
PubMed search [1] [2]

Sodium/glucose cotransporter 1 is a protein that in humans is encoded by the SLC5A1 gene.[1][2]

Function[edit]

Glucose transporters are integral membrane proteins that mediate the transport of glucose and structurally related substances across cellular membranes. Two families of glucose transporter have been identified: the facilitated diffusion glucose transporter family (GLUT family), also known as 'uniporters,' and the sodium-dependent glucose transporter family (SGLT family), also known as 'cotransporters' or 'symporters' (Wright et al., 1994). The SLC5A1 gene encodes a protein that is involved in the active transport of glucose and galactose into eukaryotic and some prokaryotic cells. [supplied by OMIM][2]

Cloning of the sodium-glucose cotransporter SGLT1[edit]

Co-transport proteins of mammalian cell membranes had eluded efforts of purification with classical biochemical methods until the late 1980s. These proteins had proven difficult to isolate because they contain hydrophilic and hydrophobic sequences and exist in membranes only in very low abundance (<0.2% of membrane proteins). The rabbit form of SGLT1 is the first mammalian co-transport protein ever to be cloned and sequenced, and this scientific break-through was reported in 1987.[3] To circumvent the difficulties with traditional isolation methods, Swiss-born biochemist Matthias Hediger and his collaborators at UCLA used a novel technique of expression cloning. They size-fractionated large amounts of rabbit intestinal mRNA with a preparative gel electrophoresis device developed by Hediger. These size fractions were then sequentially injected into Xenopus oocytes to ultimately find the RNA species that induced the expression of sodium-glucose cotransport.[4]

See also[edit]

Interactions[edit]

SLC5A1 has been shown to interact with PAWR.[5]

References[edit]

  1. ^ Turk E, Martin MG, Wright EM (June 1994). "Structure of the human Na+/glucose cotransporter gene SGLT1". J Biol Chem 269 (21): 15204–9. PMID 8195156. 
  2. ^ a b "Entrez Gene: SLC5A1 solute carrier family 5 (sodium/glucose cotransporter), member 1". 
  3. ^ by Stice L, Steven Ph.D.
  4. ^ Hediger MA, Coady MJ, Ikeda TS, Wright EM (1987). "Expression cloning and cDNA sequencing of the Na+/glucose co-transporter". Nature 330 (6146): 379–81. doi:10.1038/330379a0. PMID 2446136. 
  5. ^ Xie J, Guo Q (July 2004). "Par-4 inhibits choline uptake by interacting with CHT1 and reducing its incorporation on the plasma membrane". J. Biol. Chem. 279 (27): 28266–75. doi:10.1074/jbc.M401495200. PMID 15090548. 

Further reading[edit]

This article incorporates text from the United States National Library of Medicine, which is in the public domain.