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The testosterone derivative [[estradiol]] (an estrogen) is known to correlate with aggression in male mice.<ref name="pmid18280561">{{cite journal | author = Soma KK, Scotti MA, Newman AE, Charlier TD, Demas GE | title = Novel mechanisms for neuroendocrine regulation of aggression | journal = Front Neuroendocrinol | volume = 29 | issue = 4 | pages = 476–89 |date=October 2008 | pmid = 18280561 | doi = 10.1016/j.yfrne.2007.12.003 | url = }}</ref> Moreover, the conversion of testosterone to estradiol regulates male aggression in [[sparrows]] during breeding season.<ref name="pmid11016791">{{cite journal | author = Soma KK, Sullivan KA, Tramontin AD, Saldanha CJ, Schlinger BA, Wingfield JC | title = Acute and chronic effects of an aromatase inhibitor on territorial aggression in breeding and nonbreeding male song sparrows | journal = J. Comp. Physiol. A | volume = 186 | issue = 7–8 | pages = 759–69 | year = 2000 | pmid = 11016791 | doi = 10.1007/s003590000129 }}</ref>
The testosterone derivative [[estradiol]] (an estrogen) is known to correlate with aggression in male mice.<ref name="pmid18280561">{{cite journal | author = Soma KK, Scotti MA, Newman AE, Charlier TD, Demas GE | title = Novel mechanisms for neuroendocrine regulation of aggression | journal = Front Neuroendocrinol | volume = 29 | issue = 4 | pages = 476–89 |date=October 2008 | pmid = 18280561 | doi = 10.1016/j.yfrne.2007.12.003 | url = }}</ref> Moreover, the conversion of testosterone to estradiol regulates male aggression in [[sparrows]] during breeding season.<ref name="pmid11016791">{{cite journal | author = Soma KK, Sullivan KA, Tramontin AD, Saldanha CJ, Schlinger BA, Wingfield JC | title = Acute and chronic effects of an aromatase inhibitor on territorial aggression in breeding and nonbreeding male song sparrows | journal = J. Comp. Physiol. A | volume = 186 | issue = 7–8 | pages = 759–69 | year = 2000 | pmid = 11016791 | doi = 10.1007/s003590000129 }}</ref>

===Sports Fans===
World Cup Soccer Fans were assayed for testosterone before and after the Brazil-Italy final. Brazil won on penalty kicks. 11/12 Brazil fans showed an increase in testosterone, whereas 9 of 9 Italian fans showed a decrease. This shows that behavior and/or perception have the ability to considerably affect hormone levels.<ref>http://www.ncbi.nlm.nih.gov/pubmed/9811365</ref>


== Medical uses ==
== Medical uses ==

Revision as of 21:20, 24 February 2014

Testosterone
Clinical data
Trade namesAndroderm, Delatestryl
AHFS/Drugs.comMonograph
Pregnancy
category
Routes of
administration		Intramuscular injection, transdermal (cream, gel, or patch), sub-'Q' pellet
ATC code
Legal status
Legal status
Pharmacokinetic data
Bioavailabilitylow (due to extensive first pass metabolism)
MetabolismLiver, Testis and Prostate
Elimination half-life2–4 h
ExcretionUrine (90%), feces (6%)
Identifiers
  • (8R,9S,10R,13S,14S,17S)- 17-hydroxy-10,13-dimethyl- 1,2,6,7,8,9,11,12,14,15,16,17- dodecahydrocyclopenta[a]phenanthren-3-one
CAS Number
  • 58-22-0 checkY
    57-85-2 (propionate ester)
PubChem CID
IUPHAR/BPS
DrugBank
ChemSpider
UNII
KEGG
ChEBI
ChEMBL
CompTox Dashboard (EPA)
ECHA InfoCard100.000.336 Edit this at Wikidata
Chemical and physical data
FormulaC19H28O2
Molar mass288.42 g·mol−1
3D model (JSmol)
Specific rotation+110.2°
Melting point155 °C (311 °F)
  • O=C4\C=C2/[C@]([C@H]1CC[C@@]3([C@@H](O)CC[C@H]3[C@@H]1CC2)C)(C)CC4
  • InChI=1S/C19H28O2/c1-18-9-7-13(20)11-12(18)3-4-14-15-5-6-17(21)19(15,2)10-8-16(14)18/h11,14-17,21H,3-10H2,1-2H3/t14-,15-,16-,17-,18-,19-/m0/s1 checkY
  • Key:MUMGGOZAMZWBJJ-DYKIIFRCSA-N checkY
 ☒NcheckY (what is this?)  (verify)

Testosterone is a steroid hormone from the androgen group and is found in mammals, reptiles,[2] birds,[3] and other vertebrates. In mammals, testosterone is secreted primarily in the testicles of males and the ovaries of females, although small amounts are also secreted by the adrenal glands. It is the principal male sex hormone and an anabolic steroid.

In men, testosterone plays a key role in the development of male reproductive tissues such as the testis and prostate as well as promoting secondary sexual characteristics such as increased muscle, bone mass, and the growth of body hair.[4] In addition, testosterone is essential for health and well-being[5] as well as the prevention of osteoporosis.[6]

On average, in adult males, levels of testosterone are about 7–8 times as great as the in adult females,[7] but, as the metabolic consumption of testosterone in males is greater, the daily production is about 20 times greater in men.[8][9] Females are also more sensitive to the hormone.[10] Testosterone is observed in most vertebrates. Fish make a slightly different form called 11-ketotestosterone.[11] Its counterpart in insects is ecdysone.[12] These ubiquitous steroids suggest that sex hormones have an ancient evolutionary history.[13]

Health effects

In general, androgens promote protein synthesis and growth of those tissues with androgen receptors. Testosterone effects can be classified as virilizing and anabolic, though the distinction is somewhat artificial, as many of the effects can be considered both.

Testosterone effects can also be classified by the age of usual occurrence. For postnatal effects in both males and females, these are mostly dependent on the levels and duration of circulating free testosterone.

Before birth

The prenatal androgen effects occur during two different stages. Between 4 and 6 weeks of the gestation.

During the second trimester, androgen level is associated with gender formation.[14] This period affects the femininization or masculinization of the fetus and can be a better predictor of feminine or mascular behaviours such as sex typed behaviour than an adult's own levels. A mother's testosterone level during pregnancy is correlated with her daughter's sex-typical behavior as an adult, and the correlation is even stronger than with the daughter's own adult testosterone level.[15]

Early infancy

Early infancy androgen effects are the least understood. In the first weeks of life for male infants, testosterone levels rise. The levels remain in a pubertal range for a few months, but usually reach the barely detectable levels of childhood by 4–6 months of age.[16][17] The function of this rise in humans is unknown. It has been speculated that "brain masculinization" is occurring since no significant changes have been identified in other parts of the body.[18] It is interesting to note that the male brain is masculinized by the aromatization of testosterone into estrogen, which crosses the blood–brain barrier and enters the male brain, whereas female fetuses have alpha-fetoprotein, which binds the estrogen so that female brains are not affected.[19]

Pre-peripubertal

Pre- Peripubertal effects are the first observable effects of rising androgen levels at the end of childhood, occurring in both boys and girls.

Pubertal

Pubertal effects begin to occur when androgen has been higher than normal adult female levels for months or years. In males, these are usual late pubertal effects, and occur in women after prolonged periods of heightened levels of free testosterone in the blood.

Adult

Adult testosterone effects are more clearly demonstrable in males than in females, but are likely important to both sexes. Some of these effects may decline as testosterone levels decrease in the later decades of adult life.

Reference ranges for blood tests, showing adult male testosterone levels in light blue at center-left.

Biological uses

Cancer prevention and health risks

  • Testosterone does not cause or produce deleterious effects on prostate cancer. In people who have undergone testosterone deprivation therapy, testosterone increases beyond the castrate level have been shown to increase the rate of spread of an existing prostate cancer.[25][26][27]
  • Recent studies have shown conflicting results concerning the importance of testosterone in maintaining cardiovascular health.[28][29] Nevertheless, maintaining normal testosterone levels in elderly men has been shown to improve many parameters that are thought to reduce cardiovascular disease risk, such as increased lean body mass, decreased visceral fat mass, decreased total cholesterol, and glycemic control.[30]
  • Under dominance challenge, may play a role in the regulation of the fight-or-flight response[31]
  • Men whose testosterone levels are slightly above average are less likely to have high blood pressure, less likely to experience a heart attack, less likely to be obese, and less likely to rate their own health as fair or poor. However, high testosterone men are more likely to report one or more injuries, more likely to consume five or more alcoholic drinks in a day, more likely to have had a sexually transmitted infection, and more likely to smoke.[32]

Romantic relationships and fatherhood

Falling in love decreases men's testosterone levels while increasing women's testosterone levels. There has been speculation that these changes in testosterone result in the temporary reduction of differences in behavior between the sexes.[33] However, it is suggested that after the "honeymoon phase" ends—some one to three years into a relationship—this change in testosterone levels is no longer apparent.[33] Fatherhood also decreases testosterone levels in men, suggesting that the resulting emotional and behavioral changes promote paternal care.[34] Men who produce less testosterone are more likely to be in a relationship[35] and/or married,[36] and men who produce more testosterone are more likely to divorce;[36] however, causality cannot be determined in this relationship. Marriage or commitment could cause a decrease in testosterone levels.[37] Single men who have not had relationship experience have lower testosterone levels than single men with experience. It is suggested that these single men with prior experience are in a more competitive state than their non-experienced counterparts.[38] Married men who engage in bond-maintenance activities such as spending the day with their spouse/and or child have no different testosterone levels compared to times when they do not engage in such activities. Collectively, these results suggest that the presence of competitive activities rather than bond-maintenance activities are more relevant to changes in testosterone levels.[39]

Men who produce more testosterone are more likely to engage in extramarital sex.[36] Testosterone levels do not rely on physical presence of a partner for men engaging in relationships (same-city vs. long-distance), men have similar testosterone levels across the board.[35] Physical presence may be required for women who are in relationships for the testosterone–partner interaction, where same-city partnered women have lower testosterone levels than long-distance partnered women.[40]

Testosterone and sexual arousal

See also: Hormones and sexual arousal

It has been found that when testosterone and endorphins in ejaculated semen meet the cervical wall after sexual intercourse, females receive a spike in testosterone, endorphin, and oxytocin levels, and males after orgasm during copulation experience an increase in endorphins and a marked increase in oxytocin levels. This adds to the hospitable physiological environment in the female internal reproductive tract for conceiving, and later for nurturing the conceptus in the pre-embryonic stages, and stimulates feelings of love, desire, and paternal care in the male (this is the only time male oxytocin levels rival a female's).[33]

Testosterone levels follow a nyctohemeral rhythm that peaks early each day, regardless of sexual activity.[41]

There are positive correlations between positive orgasm experience in women and testosterone levels where relaxation was a key perception of the experience. There is no correlation between testosterone and men's perceptions of their orgasm experience, and also no correlation between higher testosterone levels and greater sexual assertiveness in either sex.[42]

An increase in testosterone levels has also been found to occur in both men and women who have masturbation-induced orgasms.[43][44]

Mammalian studies

Studies conducted on rats have indicated that their degree of sexual arousal is sensitive to reductions in testosterone. When testosterone-deprived rats were given medium levels of testosterone, their sexual behaviors (copulation, partner preference, etc.) resumed, but not when given low amounts of the same hormone. Therefore, these mammals may provide a model for studying clinical populations among humans suffering from sexual arousal deficits such as hypoactive sexual desire disorder.[45]

In one study, almost every mammalian species examined demonstrated a marked increase in a male's testosterone level upon encountering a novel female. P.J. James et al. investigated the role of genotype on such so-called reflexive testosterone increases in male mice. They also concluded that this response is related to the male's initial level of sexual arousal.[46]

In non-human primates it has been suggested that testosterone in puberty stimulates sexual motivation, which allows the primate to increasingly seek out sexual experiences with females and thus creates a sexual preference for females.[47] Some research has also indicated that if testosterone is eliminated in an adult male human or other adult male primate's system, its sexual motivation decreases, but there is no corresponding decrease in ability to engage in sexual activity (mounting, ejaculating, etc.).[47]

Male sexual arousal

Higher levels of testosterone were associated with periods of sexual activity within subjects, but between subjects testosterone levels were higher for less sexually active individuals.[48] Men who have sexual encounters with unfamiliar or multiple partners experience large increases of testosterone the morning after.[49]

Men who watch a sexually explicit movie have an average increase of 35% in testosterone, peaking at 60–90 minutes after the end of the film, but no increase is seen in men who watch sexually neutral films.[50] Men who watch sexually explicit films also report increased optimism and decreased exhaustion.[51] Previous research has found a link between relaxation following sexual arousal and testosterone levels.[52]

A 2002 study found that testosterone increased in heterosexual men after having had a brief conversation with a woman, and stayed the same after a conversation with a man. The increase in testosterone levels was associated with the degree that the women thought the men were trying to impress them.[53]

Men's levels of testosterone, a hormone known to affect men's mating behaviour, changes depending on whether they are exposed to an ovulating or nonovulating woman's body odour. Men who are exposed to scents of ovulating women maintained a stable testosterone level that was higher than the testosterone level of men exposed to nonovulation cues. Testosterone levels and sexual arousal in men are heavily aware of hormone cycles in females.[54] This may be linked to the ovulatory shift hypothesis,[55] where males are adapted to respond to the ovulation cycles of females by sensing when they are most fertile and whereby females look for preferred male mates when they are the most fertile; both actions may be driven by hormones.

In a 1991 study, males were exposed to either visual or auditory erotic stimuli and asked to complete a cognitive task, where the number of errors on the task indicated how distracted the participant was by the stimuli. It concluded that men with lower thresholds for sexual arousal have a greater likelihood to attend to sexual information and that testosterone may have an impact by enhancing their attention to the relevant stimuli.[56]

Sperm competition theory: Testosterone levels are shown to increase as a response to previously neutral stimuli when conditioned to become sexual in male rats.[57] This reaction engages penile reflexes (such as erection and ejaculation) that aid in sperm competition when more than one male is present in mating encounters, allowing for more production of successful sperm and a higher chance of reproduction.

Female sexual arousal

Androgens may modulate the physiology of vaginal tissue and contribute to female genital sexual arousal.[58] Women's level of testosterone is higher when measured pre-intercourse vs pre-cuddling, as well as post-intercourse vs post-cuddling.[59] There is a time lag effect when testosterone is administered, on genital arousal in women. In addition, a continuous increase in vaginal sexual arousal may result in higher genital sensations and sexual appetitive behaviors.[60]

When females have a higher baseline level of testosterone, they have higher increases in sexual arousal levels but smaller increases in testosterone, indicating a ceiling effect on testosterone levels in females. Sexual thoughts also change the level of testosterone but not level of cortisol in the female body, and hormonal contraceptives may have an impact on the variation in testosterone response to sexual thoughts.[61]

Testosterone may prove to be an effective treatment in female sexual arousal disorders.[62] Currently there is no FDA approved androgen preparation for the treatment of androgen insufficiency, however it has been used off-label to treat low libido and sexual dysfunction in older women. Testosterone may be a treatment for postmenopausal women as long as they are effectively estrogenized.[62]

Behavior and personality

Testosterone levels play a major role in risk-taking during financial decisions.[63][64]

A 2009 study of 25 male subjects found that men with artificially raised testosterone were 27% less generous while playing a test game than they were at their normal testosterone level. The authors concluded that "What we have found is that T[estosterone] appears to play a role inducing men to change from being selfless to being selfish."[65]

Brain

As testosterone affects the entire body (often by enlarging; males have bigger hearts, lungs, liver, etc.), the brain is also affected by this "sexual" differentiation;[14] the enzyme aromatase converts testosterone into estradiol that is responsible for masculinization of the brain in male mice. In humans, masculinization of the fetal brain appears, by observation of gender preference in patients with congenital diseases of androgen formation or androgen receptor function, to be associated with functional androgen receptors.[66]

There are some differences between a male and female brain (possibly the result of different testosterone levels), one of them being size: the male human brain is, on average, larger.[67] In a Danish study from 2003, men were found to have a total myelinated fiber length of 176,000 km at the age of 20, whereas in women the total length was 149,000 km (approx. 15% less).[68]

A study conducted in 1996 found no immediate short term effects on mood or behavior from the administration of supraphysiologic doses of testosterone for 10 weeks on 43 healthy men.[20] Another study found a correlation between testosterone and risk tolerance in career choice among women.[69][70]

The literature suggests that attention, memory, and spatial ability are key cognitive functions affected by testosterone in humans. Preliminary evidence suggests that low testosterone levels may be a risk factor for cognitive decline and possibly for dementia of the Alzheimer's type,[71][72][73][74] a key argument in life extension medicine for the use of testosterone in anti-aging therapies. Much of the literature, however, suggests a curvilinear or even quadratic relationship between spatial performance and circulating testosterone,[75] where both hypo- and hypersecretion (deficient- and excessive-secretion) of circulating androgens have negative effects on cognition.

Aggression and criminality

See also: Aggression § Testosterone, and biosocial criminology

The "evolutionary neuroandrogenic theory" focuses on the hormone testosterone as a factor influencing aggression and criminality and being evolutionarily beneficial during certain forms of competition. In most species, males are more aggressive than females. Castration of males usually has a pacifying effect on their aggressive behavior. In humans, males engage in crime and especially violent crime more than females. Their involvement in crime usually rises in the early teens to mid teens, at the same time as testosterone levels rise. Research on the relationship between testosterone and aggression is difficult, since the only reliable measurement of brain testosterone is by a lumbar puncture that is not done for research purposes. Studies therefore have often instead used more unreliable measurements from blood or saliva. [citation needed]

Most studies support a link between adult criminality and testosterone, although the relationship is modest if examined separately for each sex. Nearly all studies of juvenile delinquency and testosterone are not significant. Most studies have also found testosterone to be associated with behaviors or personality traits linked with criminality such as antisocial behavior and alcoholism. Many studies have also been done on the relationship between more general aggressive behavior/feelings and testosterone. About half the studies have found a relationship and about half no relationship.[76]

The testosterone derivative estradiol (an estrogen) is known to correlate with aggression in male mice.[77] Moreover, the conversion of testosterone to estradiol regulates male aggression in sparrows during breeding season.[78]

Sports Fans

World Cup Soccer Fans were assayed for testosterone before and after the Brazil-Italy final. Brazil won on penalty kicks. 11/12 Brazil fans showed an increase in testosterone, whereas 9 of 9 Italian fans showed a decrease. This shows that behavior and/or perception have the ability to considerably affect hormone levels.[79]

Medical uses

The original and primary use of testosterone is for the treatment of males with too little or no natural endogenous testosterone production—males with hypogonadism. Appropriate use for this purpose is legitimate hormone replacement therapy (testosterone replacement therapy [TRT]), which maintains serum testosterone levels in the normal range.

Testosterone has also been given for many other conditions and purposes besides replacement. Examples include reducing infertility, correcting lack of libido or erectile dysfunction, correcting osteoporosis, encouraging penile enlargement, encouraging height growth, encouraging bone marrow stimulation and reversing the effects of anemia, and even appetite stimulation. By the late 1940s, testosterone was being touted as an anti-aging wonder drug (e.g., see Paul de Kruif's The Male Hormone).[80] Decline of testosterone production with age has led to interest in androgen replacement therapy.[81]

To take advantage of its virilizing effects, testosterone is often administered to transsexual men as part of the hormone replacement therapy,[82] with a "target level" of the normal male testosterone level. Likewise, transsexual women are sometimes prescribed anti-androgens to decrease the level of testosterone in the body and allow for the effects of estrogen to develop.

Testosterone patches are effective at treating low libido in post-menopausal women.[83] Low libido may also occur as a symptom or outcome of hormonal contraceptive use. Women may also use testosterone therapies to treat or prevent loss of bone density and muscle mass and to treat certain kinds of depression and low energy state. Women on testosterone therapies may experience an increase in body weight without an increase in body fat due to changes in bone and muscle density. The undesired effects of testosterone therapy in women are typically controlled with symptomatic treatments, such as hair removal and topical acne therapy. There is a theoretical risk that testosterone therapy increases the risk of breast or gynaecological cancers, and further research is needed to define any such risks more clearly.[83]

Appropriate testosterone therapy may improve the management of type 2 diabetes.[84] Low testosterone has been associated with the development of Alzheimer's disease.[73][74] A small trial in 2005 showed mixed results in using testosterone to combat the effects of aging.[85]

Large-scale trials to assess the efficiency and long-term safety of testosterone are still lacking.[86]

Hormone replacement therapy

Main article: Androgen replacement therapy

Testosterone levels decline gradually with age in human beings. The clinical significance of this decrease is debated (see andropause). There is disagreement about when to treat aging men with testosterone replacement therapy. The American Society of Andrology's position is that "testosterone replacement therapy in aging men is indicated when both clinical symptoms and signs suggestive of androgen deficiency and decreased testosterone levels are present."[87] The American Association of Clinical Endocrinologists says "Hypogonadism is defined as a free testosterone level that is below the lower limit of normal for young adult control subjects. Previously, age-related decreases in free testosterone were once accepted as normal. Currently, they are not considered normal. Patients with borderline testosterone levels warrant a clinical trial of testosterone."[88]

There is not total agreement on the threshold of testosterone value below which a man would be considered hypogonadal. (Currently, there are no standards as to when to treat women.) Testosterone can be measured as "free" (that is, bioavailable and unbound) or, more commonly, "total" (including the percentage that is chemically bound and unavailable). In the United States, male total testosterone levels below 300 ng/dL from a morning serum sample are generally considered low.[89] Identification of inadequate testosterone in an aging male by symptoms alone can be difficult.

Adverse effects of testosterone supplementation include minor side-effects such as acne and oily skin, and more significant complications such as increased hematocrit, which can require venipuncture in order to treat, exacerbation of sleep apnea and acceleration of pre-existing prostate cancer growth in individuals having undergone androgen deprivation. Another adverse effect may be significant hair loss and/or thinning of the hair. This may be prevented with 5-alpha reductase inhibitors ordinarily used for the treatment of benign prostatic hyperplasia such as finasteride or dutasteride. Exogenous testosterone also causes suppression of spermatogenesis and can lead to infertility.[90]

Insufficiency

Further information: Hypogonadism

Testosterone insufficiency (also termed hypotestosteronism or hypotestosteronemia) is an abnormally low testosterone production. It may occur because of testicular dysfunction (primary hypogonadism) or hypothalamic-pituitary dysfunction (secondary hypogonadism) and may be congenital or acquired.[91] An acquired form of hypotestosteronism is the decline in testosterone levels that occurs by aging, sometimes called "andropause" in men, as a comparison to the decline in estrogen that comes with menopause in women. In Western countries, average testosterone levels are receding in men of all ages.[92][93] Several theories, from increases in obesity, to exposure to endocrine disruptors have been proposed as an explanation for this reduction.[94]

Non-medical use

Athletics

Testosterone can be used by an athlete in order to improve performance, but it is considered to be a form of doping in most sports. There are several application methods for testosterone, including intramuscular injections, transdermal gels and patches, and implantable pellets. Supplement of the hormone results in lower metabolic production via the Farquharson phenomenon, creating long-term dependence for improved performance level.[citation needed]

Anabolic steroids (including testosterone) have also been taken to enhance muscle development, strength, or endurance. They do so directly by increasing the muscles' protein synthesis. As a result, muscle fibers become larger and repair faster than the average person's. After a series of scandals and publicity in the 1980s (such as Ben Johnson's improved performance at the 1988 Summer Olympics), prohibitions of anabolic steroid use were renewed or strengthened by many sports organizations. Testosterone and other anabolic steroids were designated a "controlled substance" by the United States Congress in 1990, with the Anabolic Steroid Control Act.[95] The use is seen as being a seriously problematic issue in modern sport, particularly given the lengths to which athletes and professional laboratories go to in trying to conceal such abuse from sports regulators. Steroid abuse once again came into the spotlight recently as a result of the Chris Benoit double murder-suicide in 2007, however, there has been no evidence indicating steroid use as a contributing factor.[citation needed]

Detection of abuse

A number of methods for detecting testosterone use by athletes have been employed, most based on a urine test. These include the testosterone/epitestosterone ratio (normally less than 6), the testosterone/luteinizing hormone ratio and the carbon-13 / carbon-12 ratio (pharmaceutical testosterone contains less carbon-13 than endogenous testosterone). In some testing programs, an individual's own historical results may serve as a reference interval for interpretation of a suspicious finding. Another approach being investigated is the detection of the administered form of testosterone, usually an ester, in hair.[96][97][98][99]

Adverse effects

Testosterone is contraindicated in pregnancy and not recommended during breastfeeding.[100]

Whether or not testosterone therapy in men causes important adverse effects is unclear.[101] Up to the year 2010, studies had not shown an affect on the risk of death, prostate cancer or cardiovascular disease.;[101][102] two more recent studies, however, do raise concern.[103] It does increase hemoglobin levels and decrease HDL cholesterol levels but it is not clear if these changes are meaningful.[101]

Testosterone in the presence of a slow-growing cancer is assumed to increase its growth rate. However the association between testosterone supplementation and the development of prostate cancer is unproven.[104] Nevertheless physicians are cautioned about the cancer risk associated with testosterone supplementation.[105]

Fluoxymesterone and methyltestosterone are synthetic derivatives of testosterone. Methyltestosterone and fluoxymesterone are no longer prescribed by physicians, given their poor safety record.

Ethnic groups have different rates of prostate cancer.[106] Differences in sex hormones including testosterone have been suggested as an explanation for these differences.[106] This apparent paradox can be resolved by noting that prostate cancer is very common. In autopsies, 80% of 80-year-old men have prostate cancer.[107]

Biochemistry

Biosynthesis

Human steroidogenesis, showing testosterone near bottom.

Like other steroid hormones, testosterone is derived from cholesterol (see figure to the left).[108] The first step in the biosynthesis involves the oxidative cleavage of the sidechain of cholesterol by CYP11A, a mitochondrial cytochrome P450 oxidase with the loss of six carbon atoms to give pregnenolone. In the next step, two additional carbon atoms are removed by the CYP17A enzyme in the endoplasmic reticulum to yield a variety of C19 steroids.[109] In addition, the 3-hydroxyl group is oxidized by 3-β-HSD to produce androstenedione. In the final and rate limiting step, the C-17 keto group androstenedione is reduced by 17-β hydroxysteroid dehydrogenase to yield testosterone.

The largest amounts of testosterone (>95%) are produced by the testes in men.[4] It is also synthesized in far smaller quantities in women by the thecal cells of the ovaries, by the placenta, as well as by the zona reticularis of the adrenal cortex and even skin[110] in both sexes. In the testes, testosterone is produced by the Leydig cells.[111] The male generative glands also contain Sertoli cells, which require testosterone for spermatogenesis. Like most hormones, testosterone is supplied to target tissues in the blood where much of it is transported bound to a specific plasma protein, sex hormone-binding globulin (SHBG).

Regulation

Hypothalamic–pituitary–testicular axis

In males, testosterone is synthesized primarily in Leydig cells. The number of Leydig cells in turn is regulated by luteinizing hormone (LH) and follicle-stimulating hormone (FSH). In addition, the amount of testosterone produced by existing Leydig cells is under the control of LH, which regulates the expression of 17-β hydroxysteroid dehydrogenase.[112]

The amount of testosterone synthesized is regulated by the hypothalamic–pituitary–testicular axis (see figure to the right).[113] When testosterone levels are low, gonadotropin-releasing hormone (GnRH) is released by the hypothalamus, which in turn stimulates the pituitary gland to release FSH and LH. These latter two hormones stimulate the testis to synthesize testosterone. Finally, increasing levels of testosterone through a negative feedback loop act on the hypothalamus and pituitary to inhibit the release of GnRH and FSH/LH, respectively.

Environmental factors affecting testosterone levels include:

  • Weight loss may result in an increase in testosterone levels. Fat cells synthesize the enzyme aromatase, which converts testosterone, the male sex hormone, into estradiol, the female sex hormone.[114]
  • The secosteroid vitamin D in levels of 400–1000 IU/d (10–25 mcg/d) raises testosterone levels.[115]
  • Zinc deficiency lowers testosterone levels[116] but over supplementation has no effect on serum testosterone.[117]
  • Dominance challenges can, in some cases, stimulate increased testosterone release in men.[118]
  • Aging reduces testosterone release.[119]
  • Hypogonadism
  • Sleep (REM dream) increases nocturnal testosterone levels.[120]
  • Resistance training increases testosterone levels,[121] however, in older men, that increase can be avoided by protein ingestion.[122]
  • Licorice. The active ingredient in licorice root, glycyrrhizinic acid has been linked to small, clinically non-significant decreases in testosterone levels.[123] In contrast, a more recent study found that licorice administration produced a substantial testosterone decrease in a small, female-only sample.[124]
  • Natural or man-made antiandrogens including spearmint tea reduce testosterone levels.[125][126][127]

Metabolism

Approximately 7% of testosterone is reduced to 5α-dihydrotestosterone (DHT) by the cytochrome P450 enzyme 5α-reductase,[128] an enzyme highly expressed in male sex organs and hair follicles.[4] Approximately 0.3% of testosterone is converted into estradiol by aromatase (CYP19A1)[129] an enzyme expressed in the brain, liver, and adipose tissues.[4]

DHT is a more potent form of testosterone while estradiol has completely different activities (feminization) compared to testosterone (masculinization). Also, testosterone and DHT may be deactivated or cleared by enzymes that hydroxylate at the 6, 7, 15 or 16 positions.[130]

Mechanism of action

The effects of testosterone in humans and other vertebrates occur by way of two main mechanisms: by activation of the androgen receptor (directly or as DHT), and by conversion to estradiol and activation of certain estrogen receptors.[131][132]

Free testosterone (T) is transported into the cytoplasm of target tissue cells, where it can bind to the androgen receptor, or can be reduced to 5α-dihydrotestosterone (DHT) by the cytoplasmic enzyme 5-alpha reductase. DHT binds to the same androgen receptor even more strongly than testosterone, so that its androgenic potency is about 5 times that of T.[133] The T-receptor or DHT-receptor complex undergoes a structural change that allows it to move into the cell nucleus and bind directly to specific nucleotide sequences of the chromosomal DNA. The areas of binding are called hormone response elements (HREs), and influence transcriptional activity of certain genes, producing the androgen effects.

Androgen receptors occur in many different vertebrate body system tissues, and both males and females respond similarly to similar levels. Greatly differing amounts of testosterone prenatally, at puberty, and throughout life account for a share of biological differences between males and females.

The bones and the brain are two important tissues in humans where the primary effect of testosterone is by way of aromatization to estradiol. In the bones, estradiol accelerates ossification of cartilage into bone, leading to closure of the epiphyses and conclusion of growth. In the central nervous system, testosterone is aromatized to estradiol. Estradiol rather than testosterone serves as the most important feedback signal to the hypothalamus (especially affecting LH secretion).[citation needed] In many mammals, prenatal or perinatal "masculinization" of the sexually dimorphic areas of the brain by estradiol derived from testosterone programs later male sexual behavior.[citation needed]

Synthetic analogs

A number of synthetic analogs of testosterone have been developed with improved bioavailability and metabolic half life relative to testosterone. Many of these analogs have an alkyl group introduced at the C-17 position in order to prevent conjugation and hence improve oral bioavailability. These are the so-called "17-aa" (17-alkyl androgen) family of androgens such as fluoxymesterone and methyltestosterone.

Related drugs

Some drugs indirectly target testosterone as a way of treating certain conditions. For example, 5-alpha-reductase inhibitors such as finasteride inhibit the conversion of testosterone into dihydrotestosterone (DHT), a metabolite more potent than testosterone.[134] These 5-alpha-reductase inhibitors have been used to treat various conditions associated with androgens, such as androgenetic alopecia (male-pattern baldness), hirsutism, benign prostatic hyperplasia (BPH), and prostate cancer.[134] In contrast, GnRH antagonists bind to GnRH receptors in the pituitary gland, blocking the release of luteinising hormone (LH) and follicle-stimulating hormone (FSH) from the pituitary.[135] In men, the reduction in LH subsequently leads to rapid suppression of testosterone release from the testes. GnRH antagonists have been used for the treatment of prostate cancer.

Routes of administration

Vial of testosterone for intramuscular injection

There are many routes of administration for testosterone. Forms of testosterone for human administration currently available include injectable (such as testosterone cypionate or testosterone enanthate in oil),[136] oral, buccal,[137] transdermal skin patches, transdermal creams, gels,[138][139] and implantable pellets.[140] Roll-on methods and nasal sprays are currently under development.

History

A testicular action was linked to circulating blood fractions – now understood to be a family of androgenic hormones – in the early work on castration and testicular transplantation in fowl by Arnold Adolph Berthold (1803–1861).[141] Research on the action of testosterone received a brief boost in 1889, when the Harvard professor Charles-Édouard Brown-Séquard (1817–1894), then in Paris, self-injected subcutaneously a "rejuvenating elixir" consisting of an extract of dog and guinea pig testicle. He reported in The Lancet that his vigor and feeling of well-being were markedly restored but the effects were transient,[142] and Brown-Séquard's hopes for the compound were dashed. Suffering the ridicule of his colleagues, he abandoned his work on the mechanisms and effects of androgens in human beings.

In 1927, the University of Chicago's Professor of Physiologic Chemistry, Fred C. Koch, established easy access to a large source of bovine testicles — the Chicago stockyards — and recruited students willing to endure the tedious work of extracting their isolates. In that year, Koch and his student, Lemuel McGee, derived 20 mg of a substance from a supply of 40 pounds of bovine testicles that, when administered to castrated roosters, pigs and rats, remasculinized them.[143] The group of Ernst Laqueur at the University of Amsterdam purified testosterone from bovine testicles in a similar manner in 1934, but isolation of the hormone from animal tissues in amounts permitting serious study in humans was not feasible until three European pharmaceutical giants—Schering (Berlin, Germany), Organon (Oss, Netherlands) and Ciba (Basel, Switzerland)—began full-scale steroid research and development programs in the 1930s.

Leopold Ruzicka

The Organon group in the Netherlands were the first to isolate the hormone, identified in a May 1935 paper "On Crystalline Male Hormone from Testicles (Testosterone)".[144] They named the hormone testosterone, from the stems of testicle and sterol, and the suffix of ketone. The structure was worked out by Schering's Adolf Butenandt.[145][146]

The chemical synthesis of testosterone from cholesterol was achieved in August that year by Butenandt and Hanisch.[147] Only a week later, the Ciba group in Zurich, Leopold Ruzicka (1887–1976) and A. Wettstein, published their synthesis of testosterone.[148] These independent partial syntheses of testosterone from a cholesterol base earned both Butenandt and Ruzicka the joint 1939 Nobel Prize in Chemistry.[146][149] Testosterone was identified as 17β-hydroxyandrost-4-en-3-one (C19H28O2), a solid polycyclic alcohol with a hydroxyl group at the 17th carbon atom. This also made it obvious that additional modifications on the synthesized testosterone could be made, i.e., esterification and alkylation.

The partial synthesis in the 1930s of abundant, potent testosterone esters permitted the characterization of the hormone's effects, so that Kochakian and Murlin (1936) were able to show that testosterone raised nitrogen retention (a mechanism central to anabolism) in the dog, after which Allan Kenyon's group[150] was able to demonstrate both anabolic and androgenic effects of testosterone propionate in eunuchoidal men, boys, and women. The period of the early 1930s to the 1950s has been called "The Golden Age of Steroid Chemistry",[151] and work during this period progressed quickly. Research in this golden age proved that this newly synthesized compound—testosterone—or rather family of compounds (for many derivatives were developed from 1940 to 1960), was a potent multiplier of muscle, strength, and well-being.[80]

Synthesis

Preparation of testosterone from the abundant starting compound dehydroepiandrosterone () in essence involves interchanging oxidation states between C3 and C17, a process that would involve juggling protecting groups. The selectivity with which manganese dioxide oxidizes allylic hydroxyl groups simplifies that transformation.

Thus, reduction of androst-5-ene-1,17-dione () with lithium aluminum hydride reduced both 3- and 17-carbonyl groups, that atC17 cleanly to the b-epimer and the ketone at C3 to a mixture of epimers (). Treatment of the mixture of epimers with manganese dioxide oxidizes the allylic alcohol at C3, leaving that at C17 untouched, affording testosterone ().

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External links

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