SELPLG is the high affinity counter-receptor for P-selectin on myeloid cells and stimulated T lymphocytes. As such, it plays a critical role in the tethering of these cells to activated platelets or endothelia expressing P-selectin. The organization of the SELPG gene closely resembles that of CD43 and the human platelet glycoprotein GpIb-alpha both of which have an intron in the 5-prime-noncoding region, a long second exon containing the complete coding region, and TATA-less promoters.
PSGL-1 is expressed on all white blood cells and plays an important role in the recruitment of white blood cells into inflamed tissue.
White blood cells normally do not interact with the endothelium of blood vessels. However, inflammation causes the expression of cell adhesion molecules (CAM) such as P-selectin on the surface of the blood vessel wall. White blood cells present in flowing blood can interact with CAM. The first step in this interaction process is carried out by PSGL-1 interacting with P-selectin and/or E-selectin on endothelial cells and adherent platelets. This interaction results in "rolling" of the white blood cell on the endothelial cell surface followed by stable adhesion and transmigration of the white blood cell into the inflamed tissue.
Moore KL, Eaton SF, Lyons DE, et al. (1994). "The P-selectin glycoprotein ligand from human neutrophils displays sialylated, fucosylated, O-linked poly-N-acetyllactosamine.". J. Biol. Chem.269 (37): 23318–27. PMID7521878.
Veldman GM, Bean KM, Cumming DA, et al. (1995). "Genomic organization and chromosomal localization of the gene encoding human P-selectin glycoprotein ligand.". J. Biol. Chem.270 (27): 16470–5. doi:10.1074/jbc.270.27.16470. PMID7541799.
Wilkins PP, Moore KL, McEver RP, Cummings RD (1995). "Tyrosine sulfation of P-selectin glycoprotein ligand-1 is required for high affinity binding to P-selectin.". J. Biol. Chem.270 (39): 22677–80. doi:10.1074/jbc.270.39.22677. PMID7559387.
Li F, Wilkins PP, Crawley S, et al. (1996). "Post-translational modifications of recombinant P-selectin glycoprotein ligand-1 required for binding to P- and E-selectin.". J. Biol. Chem.271 (6): 3255–64. doi:10.1074/jbc.271.6.3255. PMID8621728.
Guyer DA, Moore KL, Lynam EB, et al. (1996). "P-selectin glycoprotein ligand-1 (PSGL-1) is a ligand for L-selectin in neutrophil aggregation.". Blood88 (7): 2415–21. PMID8839831.
Fuhlbrigge RC, Kieffer JD, Armerding D, Kupper TS (1997). "Cutaneous lymphocyte antigen is a specialized form of PSGL-1 expressed on skin-homing T cells.". Nature389 (6654): 978–81. doi:10.1038/40166. PMID9353122.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library.". Gene200 (1-2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID9373149.
Snapp KR, Ding H, Atkins K, et al. (1998). "A novel P-selectin glycoprotein ligand-1 monoclonal antibody recognizes an epitope within the tyrosine sulfate motif of human PSGL-1 and blocks recognition of both P- and L-selectin.". Blood91 (1): 154–64. PMID9414280.
Bennett EP, Hassan H, Mandel U, et al. (1998). "Cloning of a human UDP-N-acetyl-alpha-D-Galactosamine:polypeptide N-acetylgalactosaminyltransferase that complements other GalNAc-transferases in complete O-glycosylation of the MUC1 tandem repeat.". J. Biol. Chem.273 (46): 30472–81. doi:10.1074/jbc.273.46.30472. PMID9804815.
Wimazal F, Ghannadan M, Müller MR, et al. (2000). "Expression of homing receptors and related molecules on human mast cells and basophils: a comparative analysis using multi-color flow cytometry and toluidine blue/immunofluorescence staining techniques.". Tissue Antigens54 (5): 499–507. doi:10.1034/j.1399-0039.1999.540507.x. PMID10599889.
Epperson TK, Patel KD, McEver RP, Cummings RD (2000). "Noncovalent association of P-selectin glycoprotein ligand-1 and minimal determinants for binding to P-selectin.". J. Biol. Chem.275 (11): 7839–53. doi:10.1074/jbc.275.11.7839. PMID10713099.
Woltmann G, McNulty CA, Dewson G, et al. (2000). "Interleukin-13 induces PSGL-1/P-selectin-dependent adhesion of eosinophils, but not neutrophils, to human umbilical vein endothelial cells under flow.". Blood95 (10): 3146–52. PMID10807781.
Tsuchihashi S, Fondevila C, Shaw GD, et al. (2006). "Molecular characterization of rat leukocyte P-selectin glycoprotein ligand-1 and effect of its blockade: protection from ischemia-reperfusion injury in liver transplantation.". J Immunol176 (1): 616–24. doi:10.4049/jimmunol.176.1.616. PMID16365457.