NT5E

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5'-nucleotidase, ecto (CD73)
Available structures
PDB Ortholog search: PDBe, RCSB
Identifiers
Symbols NT5E ; CALJA; CD73; E5NT; NT; NT5; NTE; eN; eNT
External IDs OMIM129190 MGI99782 HomoloGene1895 ChEMBL: 5957 GeneCards: NT5E Gene
EC number 3.1.3.5
RNA expression pattern
PBB GE NT5E 203939 at tn.png
More reference expression data
Orthologs
Species Human Mouse
Entrez 4907 23959
Ensembl ENSG00000135318 ENSMUSG00000032420
UniProt P21589 Q61503
RefSeq (mRNA) NM_001204813 NM_011851
RefSeq (protein) NP_001191742 NP_035981
Location (UCSC) Chr 6:
85.45 – 85.5 Mb
Chr 9:
88.33 – 88.37 Mb
PubMed search [1] [2]

5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (Cluster of Differentiation 73), is an enzyme that in humans is encoded by the NT5E gene.[1] CD73 commonly serves to convert AMP to adenosine.

Function[edit]

Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.[2] Rare allelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.[3][4][5]

See also[edit]

References[edit]

  1. ^ Misumi Y, Ogata S, Ohkubo K, Hirose S, Ikehara Y (August 1990). "Primary structure of human placental 5'-nucleotidase and identification of the glycolipid anchor in the mature form". Eur. J. Biochem. 191 (3): 563–9. doi:10.1111/j.1432-1033.1990.tb19158.x. PMID 2129526. 
  2. ^ "Entrez Gene: NT5E 5'-nucleotidase, ecto (CD73)". 
  3. ^ St. Hilaire, Cynthia; Ziegler, Shira G.; Markello, Thomas C.; Brusco, Alfredo; Groden, Catherine; Gill, Fred; Carlson-Donohoe, Hannah; Lederman, Robert J.; Chen, Marcus Y.; Yang, Dan; Siegenthaler, Michael P.; Arduino, Carlo; Mancini, Cecilia; Freudenthal, Bernard; Stanescu, Horia C.; Zdebik, Anselm A.; Chaganti, R. Krishna; Nussbaum, Robert L.; Kleta, Robert; Gahl, William A.; Boehm, Manfred (2011). "NT5EMutations and Arterial Calcifications". New England Journal of Medicine 364 (5): 432–442. doi:10.1056/NEJMoa0912923. ISSN 0028-4793. 
  4. ^ Sharp, J. (March 1954). "Heredo-familial vascular and articular calcification". Ann. Rheum. Dis. 13 (1): 15–27. doi:10.1136/ard.13.1.15. PMC 1030367. PMID 13149051. 
  5. ^ Online 'Mendelian Inheritance in Man' (OMIM) 211800

Further reading[edit]

External links[edit]

This article incorporates text from the United States National Library of Medicine, which is in the public domain.