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Available structures
PDB Ortholog search: PDBe RCSB
Aliases NT5E, CALJA, CD73, E5NT, NT, NT5, NTE, eN, eNT, 5'-nucleotidase ecto
External IDs MGI: 99782 HomoloGene: 1895 GeneCards: 4907
RNA expression pattern
PBB GE NT5E 203939 at tn.png
More reference expression data
Species Human Mouse
RefSeq (mRNA)



RefSeq (protein)



Location (UCSC) Chr 6: 85.45 – 85.5 Mb Chr 9: 88.33 – 88.37 Mb
PubMed search [1] [2]
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5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (cluster of differentiation 73), is an enzyme that in humans is encoded by the NT5E gene.[3] CD73 commonly serves to convert AMP to adenosine.


Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.[4] Rare allelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.[5][6][7]

See also[edit]


  1. ^ "Human PubMed Reference:". 
  2. ^ "Mouse PubMed Reference:". 
  3. ^ Misumi Y, Ogata S, Ohkubo K, Hirose S, Ikehara Y (August 1990). "Primary structure of human placental 5'-nucleotidase and identification of the glycolipid anchor in the mature form". Eur. J. Biochem. 191 (3): 563–9. doi:10.1111/j.1432-1033.1990.tb19158.x. PMID 2129526. 
  4. ^ "Entrez Gene: NT5E 5'-nucleotidase, ecto (CD73)". 
  5. ^ St. Hilaire, Cynthia; Ziegler, Shira G.; Markello, Thomas C.; Brusco, Alfredo; Groden, Catherine; Gill, Fred; Carlson-Donohoe, Hannah; Lederman, Robert J.; Chen, Marcus Y.; Yang, Dan; Siegenthaler, Michael P.; Arduino, Carlo; Mancini, Cecilia; Freudenthal, Bernard; Stanescu, Horia C.; Zdebik, Anselm A.; Chaganti, R. Krishna; Nussbaum, Robert L.; Kleta, Robert; Gahl, William A.; Boehm, Manfred (2011). "NT5EMutations and Arterial Calcifications". New England Journal of Medicine. 364 (5): 432–442. doi:10.1056/NEJMoa0912923. ISSN 0028-4793. PMC 3049958free to read. PMID 21288095. 
  6. ^ Sharp, J. (March 1954). "Heredo-familial vascular and articular calcification". Ann. Rheum. Dis. 13 (1): 15–27. doi:10.1136/ard.13.1.15. PMC 1030367free to read. PMID 13149051. 
  7. ^ Online 'Mendelian Inheritance in Man' (OMIM) 211800

Further reading[edit]

External links[edit]

This article incorporates text from the United States National Library of Medicine, which is in the public domain.