SPI1

SPI1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1PUE
Identifiers
Aliases	SPI1, OF, PU.1, SFPI1, SPI-1, SPI-A, Spi-1 proto-oncogene, AGM10
External IDs	OMIM: 165170; MGI: 98282; HomoloGene: 2346; GeneCards: SPI1; OMA:SPI1 - orthologs
Gene location (Human) Chr. Chromosome 11 (human) Band 11p11.2 Start 47,354,860 bp End 47,378,547 bp
Gene location (Mouse) Chr. Chromosome 2 (mouse) Band 2 E1|2 50.44 cM Start 90,912,735 bp End 90,946,101 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in granulocyte monocyte right lung upper lobe of left lung spleen blood bone marrow cells appendix right coronary artery lymph node Top expressed in granulocyte tibiofemoral joint mesenteric lymph nodes stroma of bone marrow spleen blood medulla of thymus embryo lip ankle joint More reference expression data BioGPS More reference expression data
Gene ontology Molecular function DNA binding sequence-specific DNA binding DNA-binding transcription activator activity, RNA polymerase II-specific RNA polymerase II cis-regulatory region sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA-binding transcription repressor activity, RNA polymerase II-specific protein binding RNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding DNA-binding transcription factor activity NFAT protein binding DNA-binding transcription factor activity, RNA polymerase II-specific transcription factor binding Cellular component nucleus nucleoplasm transcription regulator complex Biological process myeloid leukocyte differentiation regulation of transcription, DNA-templated cellular response to ethanol somatic stem cell population maintenance lymphocyte differentiation regulation of erythrocyte differentiation myeloid dendritic cell differentiation negative regulation of transcription by RNA polymerase II negative regulation of MHC class II biosynthetic process lymphoid progenitor cell differentiation transcription, DNA-templated macrophage differentiation anatomical structure regression negative regulation of histone H4 acetylation negative regulation of gene expression, epigenetic vasculature development granulocyte differentiation apoptotic process involved in blood vessel morphogenesis histone H3 acetylation erythrocyte differentiation hypermethylation of CpG island positive regulation of pri-miRNA transcription by RNA polymerase II positive regulation of transcription by RNA polymerase II negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated regulation of transcription by RNA polymerase II pri-miRNA transcription by RNA polymerase II cell differentiation interleukin-6-mediated signaling pathway Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 6688 20375 Ensembl ENSG00000066336 ENSMUSG00000002111 UniProt P17947 P17433 RefSeq (mRNA) NM_001080547 NM_003120 NM_011355 NM_001378898 NM_001378899 RefSeq (protein) NP_001074016 NP_003111 NP_035485 NP_001365827 NP_001365828 Location (UCSC) Chr 11: 47.35 – 47.38 Mb Chr 2: 90.91 – 90.95 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

Transcription factor PU.1 is a protein that in humans is encoded by the SPI1 gene.

Function

This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found on enhancers of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.

Interactions

SPI1 has been shown to interact with:

• FUS,
• GATA2,
• IRF4, and
• NONO.

See also

• Spi-1 (PU.1) 5' UTR regulatory element

Further reading

• Klemsz MJ, McKercher SR, Celada A, Van Beveren C, Maki RA (1990). "The macrophage and B cell-specific transcription factor PU.1 is related to the ets oncogene". Cell. 61 (1): 113–24. doi:10.1016/0092-8674(90)90219-5. PMID 2180582.
• Nguyen VC, Ray D, Gross MS, de Tand MF, Frézal J, Moreau-Gachelin F (1990). "Localization of the human oncogene SPI1 on chromosome 11, region p11.22". Hum. Genet. 84 (6): 542–6. doi:10.1007/bf00210807. PMID 2338340.
• Chen H, Ray-Gallet D, Zhang P, Hetherington CJ, Gonzalez DA, Zhang DE, Moreau-Gachelin F, Tenen DG (1995). "PU.1 (Spi-1) autoregulates its expression in myeloid cells". Oncogene. 11 (8): 1549–60. PMID 7478579.
• Nagulapalli S, Pongubala JM, Atchison ML (1995). "Multiple proteins physically interact with PU.1. Transcriptional synergy with NF-IL6 beta (C/EBP delta, CRP3)". J. Immunol. 155 (9): 4330–8. PMID 7594592.
• Pongubala JM, Atchison ML (1995). "Activating transcription factor 1 and cyclic AMP response element modulator can modulate the activity of the immunoglobulin kappa 3' enhancer". J. Biol. Chem. 270 (17): 10304–13. doi:10.1074/jbc.270.17.10304. PMID 7730336.{{cite journal}}: CS1 maint: unflagged free DOI (link)
• Hromas R, Orazi A, Neiman RS, Maki R, Van Beveran C, Moore J, Klemsz M (1993). "Hematopoietic lineage- and stage-restricted expression of the ETS oncogene family member PU.1". Blood. 82 (10): 2998–3004. PMID 8219191.
• Hagemeier C, Bannister AJ, Cook A, Kouzarides T (1993). "The activation domain of transcription factor PU.1 binds the retinoblastoma (RB) protein and the transcription factor TFIID in vitro: RB shows sequence similarity to TFIID and TFIIB". Proc. Natl. Acad. Sci. U.S.A. 90 (4): 1580–4. doi:10.1073/pnas.90.4.1580. PMC 45918. PMID 8434021.
• Hallier M, Tavitian A, Moreau-Gachelin F (1996). "The transcription factor Spi-1/PU.1 binds RNA and interferes with the RNA-binding protein p54nrb". J. Biol. Chem. 271 (19): 11177–81. doi:10.1074/jbc.271.19.11177. PMID 8626664.{{cite journal}}: CS1 maint: unflagged free DOI (link)
• Bassuk AG, Anandappa RT, Leiden JM (1997). "Physical interactions between Ets and NF-kappaB/NFAT proteins play an important role in their cooperative activation of the human immunodeficiency virus enhancer in T cells". J. Virol. 71 (5): 3563–73. PMC 191503. PMID 9094628.
• Li SL, Valente AJ, Zhao SJ, Clark RA (1997). "PU.1 is essential for p47(phox) promoter activity in myeloid cells". J. Biol. Chem. 272 (28): 17802–9. doi:10.1074/jbc.272.28.17802. PMID 9211934.{{cite journal}}: CS1 maint: unflagged free DOI (link)
• Tsukada J, Misago M, Serino Y, Ogawa R, Murakami S, Nakanishi M, Tonai S, Kominato Y, Morimoto I, Auron PE, Eto S (1997). "Human T-cell leukemia virus type I Tax transactivates the promoter of human prointerleukin-1beta gene through association with two transcription factors, nuclear factor-interleukin-6 and Spi-1". Blood. 90 (8): 3142–53. PMID 9376596.
• Hallier M, Lerga A, Barnache S, Tavitian A, Moreau-Gachelin F (1998). "The transcription factor Spi-1/PU.1 interacts with the potential splicing factor TLS". J. Biol. Chem. 273 (9): 4838–42. doi:10.1074/jbc.273.9.4838. PMID 9478924.{{cite journal}}: CS1 maint: unflagged free DOI (link)
• Suzuki S, Kumatori A, Haagen IA, Fujii Y, Sadat MA, Jun HL, Tsuji Y, Roos D, Nakamura M (1998). "PU.1 as an essential activator for the expression of gp91(phox) gene in human peripheral neutrophils, monocytes, and B lymphocytes". Proc. Natl. Acad. Sci. U.S.A. 95 (11): 6085–90. doi:10.1073/pnas.95.11.6085. PMC 27589. PMID 9600921.
• Carrère S, Verger A, Flourens A, Stehelin D, Duterque-Coquillaud M (1998). "Erg proteins, transcription factors of the Ets family, form homo, heterodimers and ternary complexes via two distinct domains". Oncogene. 16 (25): 3261–8. doi:10.1038/sj.onc.1201868. PMID 9681824.
• Sato M, Morii E, Takebayashi-Suzuki K, Yasui N, Ochi T, Kitamura Y, Nomura S (1999). "Microphthalmia-associated transcription factor interacts with PU.1 and c-Fos: determination of their subcellular localization". Biochem. Biophys. Res. Commun. 254 (2): 384–7. doi:10.1006/bbrc.1998.9918. PMID 9918847.
• Brass AL, Zhu AQ, Singh H (1999). "Assembly requirements of PU.1-Pip (IRF-4) activator complexes: inhibiting function in vivo using fused dimers". EMBO J. 18 (4): 977–91. doi:10.1093/emboj/18.4.977. PMC 1171190. PMID 10022840.
• Yamamoto H, Kihara-Negishi F, Yamada T, Hashimoto Y, Oikawa T (1999). "Physical and functional interactions between the transcription factor PU.1 and the coactivator CBP". Oncogene. 18 (7): 1495–501. doi:10.1038/sj.onc.1202427. PMID 10050886.
• Rao S, Matsumura A, Yoon J, Simon MC (1999). "SPI-B activates transcription via a unique proline, serine, and threonine domain and exhibits DNA binding affinity differences from PU.1". J. Biol. Chem. 274 (16): 11115–24. doi:10.1074/jbc.274.16.11115. PMID 10196196.{{cite journal}}: CS1 maint: unflagged free DOI (link)
• Mao S, Frank RC, Zhang J, Miyazaki Y, Nimer SD (1999). "Functional and physical interactions between AML1 proteins and an ETS protein, MEF: implications for the pathogenesis of t(8;21)-positive leukemias". Mol. Cell. Biol. 19 (5): 3635–44. doi:10.1128/mcb.19.5.3635. PMC 84165. PMID 10207087.

External links

• FactorBook PU.1